Stem Cell Physiological Responses to Noninvasive Electrical Stimulation

2016 ◽  
pp. 105-134
Keyword(s):  
Stem Cell ◽  
Electrical Stimulation ◽  
Physiological Responses

scholarly journals Enriching neural stem cell and anti‐inflammatory glial phenotypes with electrical stimulation after traumatic brain injury in male rats

2021 ◽  
Author(s):  
Eunyoung Park ◽  
Johnathan G. Lyon ◽  
Melissa Alvarado‐Velez ◽  
Martha I. Betancur ◽  
Nassir Mokarram ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Brain Injury ◽  
Neural Stem Cell ◽  
Male Rats ◽  
Anti Inflammatory

scholarly journals Electrical Stimulation Decreases Dental Pulp Stem Cell Osteo-/Odontogenic Differentiation

2020 ◽  
Vol 9 (1) ◽  
pp. 162-173
Author(s):  
Karla Mychellyne Costa Oliveira ◽  
Liudmila Leppik ◽  
Khyati Keswani ◽  
Sreeraj Rajeev ◽  
Mit B. Bhavsar ◽  
...  
Keyword(s):  
Stem Cell ◽  
Electrical Stimulation ◽  
Dental Pulp ◽  
Odontogenic Differentiation ◽  
Dental Pulp Stem Cell

scholarly journals Hemogenic Endothelium-like Changes Were Derived to an Adult Human Dermal Fibroblast By Electrical Stimulation

Blood ◽  
2014 ◽  
Vol 124 (21) ◽  
pp. 5129-5129
Author(s):  
Takuji Matsuo ◽  
Ryosuke Shirasaki ◽  
Oka Yoko ◽  
Tadashi Yamamoto ◽  
Jun Ooi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Electrical Stimulation ◽  
Endothelial Cell ◽  
Cell Fate ◽  
Dermal Fibroblast ◽  
Vascular Endothelial Cell ◽  
Human Dermal Fibroblast ◽  
Vascular Endothelial ◽  
Hematopoietic Stem ◽  
Morphologic Changes

Abstract Background and Aims: We previously reported that when an adult human dermal fibroblast (HDF) was cultured with interleukin (IL)-1-beta (b) and erythropoietin (EPO), hematopoiesis-related molecules expressed. And, lymphatic duct-neogenesis genes also expressed, and vascular endothelial growth factor (VEGF)-A, and -C were produced. When anti-human VEGF-C antibody (Ab) was added to the cultures, hematopoiesis-related genes expressed; however, morphologic changes were not observed (54th ASH, 18th EHA). Reported findings on nuclear transfer reveal that electrical stimulation induces nuclear fusion and changes cell-fate. We observed the effect of electrical stimulation to IL-1-b-stimulated HDF. Materials and Methods: HDF was cultured with IL-1-b, EPO, VEGF-A, and anti-human VEGF-C Ab for 14 days. Then, cells were suspended in electrical stimulation-buffer (0.25 M d-sorbitol, 0.1 mM Ca-acetate, 0.5 mM Mg-acetate, 1 mg/mL fatty-acid-free BSA, 0.5 mM HEPES), and incubated on ice for 10 minutes. Cells were stimulated at 110V 20mA (0.2 cm electrode, gap), and were further cultured with k/o DMEM containing 20% KSR and SCF, IL-6, FL, IGF-2, and VEGF-A. Morphological changes and expressions of vascular endothelial cell-related genes, and hematopoiesis-related ones were observed. Results: When HDF was stimulated electrically, a few cells showed vascular endothelial cell-morphology after two days. When cells were further cultured in a hematopoietic stem cell-culturing condition, a part of endothelial cell-morphology changed to that of non-adherent hematopoietic cells or blastic colonies, in which increased expression-levels of SCL, CD41, GATA-2, CD34, and CD45 were accepted with significant statistical difference. Discussion: Recent reports reveal that a kind of vascular endothelial cells, called hemogenic endothelium, can convert to a hematopoietic cell, and works an important role in hematopoietic stem cell-generation. We hypothesized that when cells expressed a kind of specific transcripts, some stimulation triggerred to convert morphologic changes and cell-fate as are observed in nuclear fusion. And, our observations indicated that when IL-1-b-stimulated HDF, expressing hematopoiesis-related transcripts, were stimulated electrically, cellular morphology changed. Currently, we are precisely analyzing electrically stimulated HDF’s biological characteristics. Disclosures No relevant conflicts of interest to declare.

Peripheral nerve injury therapeutics, including electrical stimulation, stem cell treatments, and synthetic neural scaffolds, have shown promising preclinical and even clinical results with potential regenerative treatment

2021 ◽  
Author(s):  
Moataz Dowaidar
Keyword(s):  
Stem Cell ◽  
Electrical Stimulation ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Functional Recovery ◽  
Peripheral Nerve Regeneration ◽  
Clinical Results ◽  
Preclinical Research ◽  
Cell Therapies ◽  
Nerve Recovery

In the U.S., peripheral nerve injuries (PNI) harm about 22 million people. The most frequent causes and types of PNI vary by demography (civilians vs. military, geography/country). After crush injuries, functional recovery is better than after transections, and better after distal injuries than proximal ones. Despite advancements in microsurgical treatments, severe PNIs remain connected to slow recovery. This review highlights new peripheral nerve regeneration approaches (e.g. electrical stimulation, cell therapies), which may lead to a shift in PNI therapeutic paradigms in conjunction with neurotrophic agents and breakthroughs in bioscaffold engineering. It also examines how synthetic neural scaffolds can aid with peripheral nerve recovery, as well as the next generation of biomimetic neural scaffolding that can aid in tissue regeneration. Neurotrophic factor-enriched neural scaffolds, stem cell treatments, and electrical stimulation have shown promising preclinical and even clinical results. The future of peripheral nerve regeneration is bright, since a combination of the aforementioned treatments may have a synergistic impact on nerve regeneration and functional recovery in patients with PNI. Stem cell technology is improving and evolving, and it has been explored through a number of methods in preclinical research for peripheral nerve regeneration. Electrical stimulation is another interesting potential treatment for PNI that may be used to stimulate axon regeneration.

scholarly journals Conducting Polymer Mediated Electrical Stimulation Induces Multilineage Differentiation with Robust Neuronal Fate Determination of Human Induced Pluripotent Stem Cells

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 658 ◽  
Author(s):  
Eva Tomaskovic-Crook ◽  
Qi Gu ◽  
Siti N Abdul Rahim ◽  
Gordon G Wallace ◽  
Jeremy M Crook
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Electrical Stimulation ◽  
Cell Differentiation ◽  
Pluripotent Stem Cells ◽  
Stem Cell Differentiation ◽  
Chemical Inducers ◽  
Neuronal Fate ◽  
Induced Pluripotent

Electrical stimulation is increasingly being used to modulate human cell behaviour for biotechnological research and therapeutics. Electrically conductive polymers (CPs) such as polypyrrole (PPy) are amenable to in vitro and in vivo cell stimulation, being easy to synthesise with different counter ions (dopants) to augment biocompatibility and cell-effects. Extending our earlier work, which showed that CP-mediated electrical stimulation promotes human neural stem cell differentiation, here we report using electroactive PPy containing the anionic dopant dodecylbenzenesulfonate (DBS) to modulate the fate determination of human induced pluripotent stem cells (iPSCs). Remarkably, the stimulation without conventional chemical inducers resulted in the iPSCs differentiating to cells of the three germ lineages—endoderm, ectoderm, and mesoderm. The unstimulated iPSC controls remained undifferentiated. Phenotypic characterisation further showed a robust induction to neuronal fate with electrical stimulation, again without customary chemical inducers. Our findings add to the growing body of evidence supporting the use of electrical stimulation to augment stem cell differentiation, more specifically, pluripotent stem cell differentiation, and especially neuronal induction. Moreover, we have shown the versatility of electroactive PPy as a cell-compatible platform for advanced stem cell research and translation, including identifying novel mechanisms of fate regulation, tissue development, electroceuticals, and regenerative medicine.

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