Mechanobiology of Bladder Urothelial Cells

2011 ◽  
pp. 369-386 ◽  
Keyword(s):  
Urothelial Cells

150: The Effect of Epidermal Growth Factor (EGF) on Inward Potassium Rectifying Currents in Bladder Urothelial Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 41-41
Author(s):  
P. Sean Van Zijl ◽  
Yan Sun ◽  
Mingkui Chen ◽  
Marc Simard ◽  
Toby C. Chai
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Urothelial Cells ◽  
Epidermal Growth

976: Overexpression of Aurora A / STK15/ BTAK Induces Chromosomal Missegregation and Aneuploidy in Urothelial Cells

2004 ◽  
Vol 171 (4S) ◽  
pp. 258-259
Author(s):  
Noriyoshi Tanaka ◽  
Subrata Sen ◽  
Bogdan A. Czerniak ◽  
H. Barton Grossman
Keyword(s):  
Aurora A ◽  
Urothelial Cells

scholarly journals Associations between Arsenic Species in Exfoliated Urothelial Cells and Prevalence of Diabetes among Residents of Chihuahua, Mexico

2014 ◽  
Vol 122 (10) ◽  
pp. 1088-1094 ◽  
Author(s):  
Jenna M. Currier ◽  
María C. Ishida ◽  
Carmen González-Horta ◽  
Blanca Sánchez-Ramírez ◽  
Lourdes Ballinas-Casarrubias ◽  
...  
Keyword(s):  
Arsenic Species ◽  
Urothelial Cells

Electrospun PLGA and PLGA/gelatin scaffolds for tubularized urethral replacement: Studies in vitro and in vivo

2021 ◽  
pp. 088532822110309
Author(s):  
Jinhua Hu ◽  
Bin Ai ◽  
Shibo Zhu ◽  
Zhen Wang ◽  
Huimin Xia ◽  
...  
Keyword(s):  
Tensile Deformation ◽  
Canine Model ◽  
Urothelial Cells ◽  
Acellular Matrix ◽  
Urethral Strictures ◽  
Acid Copolymer ◽  
Hematoxylin Eosin ◽  
Collagen Tissue

To investigate the biocompatibility of polylactic acid-glycolic acid copolymer (PLGA) and PLGA/gelatin scaffolds and their suitability for tubular urethral replacement in a canine model. PLGA and PLGA/gelatin scaffolds was constructed by electrospinning. Microstructural differences between the scaffolds was examined by Scanning electron microscopy (SEM) followed by mechanical properties testing. Biocompatibility of the material was evaluated using SEM 4, 8, 12 and 72 h after PLGA and PLGA/gelatin scaffolds co-culture with urothelial cells. And confocal analysis was also used to showed the cell adhesive and growth at 12 h. Approximately 2 cm of the anterior urethra of twelve dogs were removed and replaced with a scaffold. After the surgery for 1 month performed urethrography and for 3 month perform hematoxylin–eosin (H&E) and Masson. The results indicated that PLGA and PLGA/gelatin scaffolds had a void microfilament structure, similar to that of normal acellular matrix tissue. And the tensile strength was decreased whereas the tensile deformation and suture retention strength was increased in PLGA/gelatin scaffolds compared to that in PLGA scaffolds Urothelial cells grew well on both scaffolds. Postoperatively, animals recovered well and urinated spontaneously. However, urethrography showed varying degrees of urethral strictures in the reconstructed urethras. H&E and Masson showed that multilayer urothelial cells were formed in both the proximal and distal segments of the reconstructed urethras but without continuity. There was a small amount of smooth muscle and blood vessels under the epithelium, but regenerative urothelial cells at the midpoint of the reconstructed segment did not continue. Lots of lymphocyte infiltration was observed under the epithelium, some collagen tissue was deposited under the neo-urethral epithelium were observed. In conclusion, PLGA and PLGA/gelatin scaffolds are not suitable for tubularized urethral replacement in the canine model.

Integrated genomic and transcriptional analysis of the in vitro evolution of telomerase-immortalized urothelial cells (TERT-NHUC)

2009 ◽  
Vol 48 (8) ◽  
pp. 694-710 ◽  
Author(s):  
Emma J. Chapman ◽  
Sarah V. Williams ◽  
Fiona M. Platt ◽  
Carolyn D. Hurst ◽  
Philip Chambers ◽  
...  
Keyword(s):  
Transcriptional Analysis ◽  
Urothelial Cells ◽  
In Vitro Evolution

DNA Content of Normal and Neoplastic Urothelial Cells

1980 ◽  
Vol 66 (4) ◽  
pp. 445-458 ◽  
Author(s):  
Mathilde E. Boon
Keyword(s):  
Dna Content ◽  
Cell Populations ◽  
Malignant Cells ◽  
Urothelial Cells ◽  
Chromatin Pattern ◽  
Normal Bladder ◽  
Well Differentiated ◽  
Dna Measurements ◽  
Combined Parameters ◽  
Umbrella Cells

In search for suitable parameters to detect neoplastic urothelial cells in Acriflavine-Feulgen-SITS stained specimen we compared the cytofluorometric DNA content with the morphology of normal urothelial cells (bladder scrapings) and neoplastic urothelial cells from grade 1, 2, 3 and 4 tumors. An individual normal urothelial cell could not be distinguished from a grade 1 tumor cell, neither morphologically nor fluorometrically. However, the shape of the histograms of DNA measurements of the cell populations of respectively normal bladder scrapings and grade 1 tumors differs. It is postulated that also morphometry of these cell populations may be of some aid to distinguish well-differentiated neoplastic cells from normal urothelial cells. Seventy-one percent of the morphologically malignant cells in the grade 2, 3 and 4 tumor samples could be identified by applying the combined parameters: high DNA content (> 5 C) and nuclear-cytoplasmic ratio (> 0.5) and all grade 2, 3 and 4 tumor samples contained cells which were objectively classified as malignant. Using the same parameters morphologically malignant cells could be distinguished from normal, polyploid umbrella cells, thus these malignant cells are detectable objectively without using chromatin pattern as parameter.

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