Molecular Genetics of Drought Resistance Response in Tomato and Related Species

2006 ◽  
pp. 261-283 ◽  
Author(s):  
Mary O’Connell ◽  
Andrea Medina ◽  
Pedro Sánchez Peña ◽  
Marcela Treviño
Keyword(s):  
Molecular Genetics ◽  
Drought Resistance ◽  
Related Species ◽  
Resistance Response

Controlled nuclear import of the transcription factor NTL6 reveals a cytoplasmic role of SnRK2.8 in the drought-stress response

2012 ◽  
Vol 448 (3) ◽  
pp. 353-363 ◽  
Author(s):  
Mi Jung Kim ◽  
Mi-Jeong Park ◽  
Pil Joon Seo ◽  
Jin-Su Song ◽  
Hie-Joon Kim ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Drought Stress ◽  
Transgenic Plants ◽  
Drought Resistance ◽  
Nuclear Import ◽  
Proteolytic Processing ◽  
Transcriptional Responses ◽  
Resistance Response ◽  
Proteolytic Activation

Controlled proteolytic activation of membrane-anchored transcription factors provides an adaptation strategy that guarantees rapid transcriptional responses to abrupt environmental stresses in both animals and plants. NTL6 is a plant-specific NAC [NAM/ATAF1/2/CUC2] transcription factor that is expressed as a dormant plasma membrane-associated form in Arabidopsis. Proteolytic processing of NTL6 is triggered by abiotic stresses and ABA (abscisic acid). In the present study, we show that NTL6 is linked directly with SnRK (Snf1-related protein kinase) 2.8-mediated signalling in inducing a drought-resistance response. SnRK2.8 phosphorylates NTL6 primarily at Thr142. NTL6 phosphorylation by SnRK2.8 is required for its nuclear import. Accordingly, a mutant NTL6 protein, in which Thr142 was mutated to an alanine, was poorly phosphorylated and failed to enter the nucleus. In accordance with the role of SnRK2.8 in drought-stress signalling, transgenic plants overproducing either NTL6 or its active form 6ΔC (35S:NTL6 and 35S:6ΔC) exhibited enhanced resistance to water-deficit conditions such as those overproducing SnRK2.8 (35S:SnRK2.8). In contrast, NTL6 RNAi (RNA interference) plants were susceptible to dehydration as observed in the SnRK2.8-deficient snrk2.8-1 mutant. Furthermore, the dehydration-resistant phenotype of 35S:NTL6 transgenic plants was compromised in 35S:NTL6 X snrk2.8-1 plants. These observations indicate that SnRK2.8-mediated protein phosphorylation, in addition to a proteolytic processing event, is important for NTL6 function in inducing a drought-resistance response.

Breeding for Drought Resistance in Rice: Physiology and Molecular Genetics Considerations

Crop Science ◽  
1997 ◽  
Vol 37 (5) ◽  
pp. 1426-1434 ◽  
Author(s):  
Henry T. Nguyen ◽  
R. Chandra Babu ◽  
A. Blum
Keyword(s):  
Molecular Genetics ◽  
Drought Resistance ◽  
Rice Physiology

Introduction

1978 ◽  
Vol 36 (3) ◽  
pp. 543-544
Author(s):  
W. Bernard
Keyword(s):  
Molecular Weight ◽  
Electron Microscope ◽  
Nucleic Acid ◽  
Gene Mapping ◽  
Molecular Genetics ◽  
Cell Biology ◽  
Gene Activity ◽  
Close Connection ◽  
Basic Information ◽  
Simple Systems

In comparison to many other fields of ultrastructural research in Cell Biology, the successful exploration of genes and gene activity with the electron microscope in higher organisms is a late conquest. Nucleic acid molecules of Prokaryotes could be successfully visualized already since the early sixties, thanks to the Kleinschmidt spreading technique - and much basic information was obtained concerning the shape, length, molecular weight of viral, mitochondrial and chloroplast nucleic acid. Later, additonal methods revealed denaturation profiles, distinction between single and double strandedness and the use of heteroduplexes-led to gene mapping of relatively simple systems carried out in close connection with other methods of molecular genetics.

Molecular Genetics Of Acoustic Tumors

1992 ◽  
Vol 25 (5) ◽  
pp. 995-1009
Author(s):  
Michael J. Lanser
Keyword(s):  
Molecular Genetics

Effects of oxygen level on metabolism and development of seedlings of Trapa natans and two ecologically related species

1992 ◽  
Vol 86 (1) ◽  
pp. 168-172 ◽  
Author(s):  
Faustino Menegus ◽  
Liliana Cattaruzza ◽  
Leonardo Scaglioni ◽  
Enzio Ragg
Keyword(s):  
Related Species ◽  
Oxygen Level ◽  
Trapa Natans
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