Moderate Thermophiles

2016 ◽  
pp. 118-137 ◽  
Keyword(s):  
Moderate Thermophiles

scholarly journals Simple efficient methods for the isolation of malate dehydrogenase from thermophilic and mesophilic bacteria

1979 ◽  
Vol 177 (2) ◽  
pp. 441-448 ◽  
Author(s):  
I P Wright ◽  
T K Sundaram
Keyword(s):  
Affinity Chromatography ◽  
Malate Dehydrogenase ◽  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Electrophoretic Analysis ◽  
Polyacrylamide Gel ◽  
Moderately Thermophilic ◽  
Extreme Thermophiles ◽  
Moderate Thermophiles ◽  
Number Of Bacteria

Malate dehydrogenase from a number of bacteria drawn from several genera and representing the mesophilic, moderately thermophilic and extremely thermophilic classes was isolated by procedures which involve only a small number of steps (in most cases only two), of which the key one is affinity chromatography on 5′-AMP–Sepharose and/or on NAD+–hexane–agarose. Electrophoretic analysis of the native enzymes in polyacrylamide gel and of the denaturated enzymes in sodium dodecyl sulphate/polyacrylamide gel revealed no significant protein impurity in the purified preparations. The yields ranged from about 40% to over 80%. The malate dehydrogenases from the extreme thermophiles and from some of the moderate thermophiles are appreciably less efficient catalytically than their mesophilic homologues.

Bioleaching of multiple metals from contaminated sediment by moderate thermophiles

2015 ◽  
Vol 97 (1-2) ◽  
pp. 47-55 ◽  
Author(s):  
Min Gan ◽  
Shiqi Jie ◽  
Mingming Li ◽  
Jianyu Zhu ◽  
Xinxing Liu
Keyword(s):  
Contaminated Sediment ◽  
Moderate Thermophiles

scholarly journals Complete Genome Sequences of Two Interactive Moderate Thermophiles,Paenibacillus napthalenovorans32O-Y andPaenibacillussp. 32O-W

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Robert R. Butler ◽  
Jia Wang ◽  
Benjamin C. Stark ◽  
Jean-François Pombert
Keyword(s):  
High Temperature ◽  
Complete Genome ◽  
Industrial Applications ◽  
Whole Genome ◽  
Genome Sequences ◽  
High Demand ◽  
Moderate Thermophiles

Microorganisms with the capability to desulfurize petroleum are in high demand with escalating restrictions currently placed on fuel purity. Thermophilic desulfurizers are particularly valuable in high-temperature industrial applications. We report the whole-genome sequences ofPaenibacillus napthalenovorans32O-Y andPaenibacillussp. 32O-W, which can and cannot, respectively, metabolize dibenzothiophene.

Ferrous Iron and Pyrite Oxidation by “Acidithiomicrobium” Species

2009 ◽  
Vol 71-73 ◽  
pp. 271-274 ◽  
Author(s):  
Carol S. Davis-Belmar ◽  
Paul R. Norris
Keyword(s):  
Mixed Culture ◽  
Yeast Extract ◽  
Ferrous Iron ◽  
Novel Species ◽  
Pyrite Oxidation ◽  
The Novel ◽  
Autotrophic Growth ◽  
Optimum Temperature ◽  
Moderate Thermophiles ◽  
Sulfur Oxidizing

Novel iron- and sulfur-oxidizing, moderate thermophiles were isolated from an acidic geothermal site and from a previously studied, pyrite-enrichment mixed culture (which also contained the related actinobacterium Acidimicrobium ferrooxidans). The novel species (proposed genus “Acidithiomicrobium”) grew autotrophically with ferrous iron at an optimum temperature of about 50°C, efficiently degraded pyrite at 55°C and also grew well autotrophically on sulfur. The extensive dissolution of pyrite during autotrophic growth contrasted with a requirement for yeast extract for significant growth of the related Acidimicrobium ferrooxidans.

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