Anaerobic Metabolism by Pseudomonas aeruginosa in Cystic Fibrosis Airway

2004 ◽  
pp. 97-118
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Anaerobic Metabolism ◽  
Cystic Fibrosis Airway

scholarly journals Pseudomonas aeruginosa biofilm formation in the cystic fibrosis airway

2008 ◽  
Vol 21 (4) ◽  
pp. 595-599 ◽  
Author(s):  
Sophie Moreau-Marquis ◽  
Bruce A. Stanton ◽  
George A. O’Toole
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Cystic Fibrosis Airway

scholarly journals A physical linkage between cystic fibrosis airway surface dehydration and Pseudomonas aeruginosa biofilms

2006 ◽  
Vol 103 (48) ◽  
pp. 18131-18136 ◽  
Author(s):  
H. Matsui ◽  
V. E. Wagner ◽  
D. B. Hill ◽  
U. E. Schwab ◽  
T. D. Rogers ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Cystic Fibrosis Airway ◽  
Physical Linkage

Adaptation of Pseudomonas aeruginosa to the cystic fibrosis airway: an evolutionary perspective

2012 ◽  
Vol 10 (12) ◽  
pp. 841-851 ◽  
Author(s):  
Anders Folkesson ◽  
Lars Jelsbak ◽  
Lei Yang ◽  
Helle Krogh Johansen ◽  
Oana Ciofu ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Evolutionary Perspective ◽  
Cystic Fibrosis Airway

scholarly journals Proteomic, Microarray, and Signature-Tagged Mutagenesis Analyses of Anaerobic Pseudomonas aeruginosa at pH 6.5, Likely Representing Chronic, Late-Stage Cystic Fibrosis Airway Conditions

2008 ◽  
Vol 190 (8) ◽  
pp. 2739-2758 ◽  
Author(s):  
Mark D. Platt ◽  
Michael J. Schurr ◽  
Karin Sauer ◽  
Gustavo Vazquez ◽  
Irena Kukavica-Ibrulj ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Late Stage ◽  
Tricarboxylic Acid Cycle ◽  
Major Step ◽  
Transport Chain ◽  
Cystic Fibrosis Airway ◽  
Molecular Machinery ◽  
Microarray Studies ◽  
Pf1 Bacteriophage

ABSTRACT Patients suffering from cystic fibrosis (CF) commonly harbor the important pathogen Pseudomonas aeruginosa in their airways. During chronic late-stage CF, P. aeruginosa is known to grow under reduced oxygen tension and is even capable of respiring anaerobically within the thickened airway mucus, at a pH of ∼6.5. Therefore, proteins involved in anaerobic metabolism represent potentially important targets for therapeutic intervention. In this study, the clinically relevant “anaerobiome” or “proteogenome” of P. aeruginosa was assessed. First, two different proteomic approaches were used to identify proteins differentially expressed under anaerobic versus aerobic conditions. Microarray studies were also performed, and in general, the anaerobic transcriptome was in agreement with the proteomic results. However, we found that a major portion of the most upregulated genes in the presence of NO3 − and NO2 − are those encoding Pf1 bacteriophage. With anaerobic NO2 −, the most downregulated genes are those involved postglycolytically and include many tricarboxylic acid cycle genes and those involved in the electron transport chain, especially those encoding the NADH dehydrogenase I complex. Finally, a signature-tagged mutagenesis library of P. aeruginosa was constructed to further screen genes required for both NO3 − and NO2 − respiration. In addition to genes anticipated to play important roles in the anaerobiome (anr, dnr, nar, nir, and nuo), the cysG and dksA genes were found to be required for both anaerobic NO3 − and NO2 − respiration. This study represents a major step in unraveling the molecular machinery involved in anaerobic NO3 − and NO2 − respiration and offers clues as to how we might disrupt such pathways in P. aeruginosa to limit the growth of this important CF pathogen when it is either limited or completely restricted in its oxygen supply.

scholarly journals The Pseudomonas aeruginosa Autoinducer 3O-C12 Homoserine Lactone Provokes Hyperinflammatory Responses from Cystic Fibrosis Airway Epithelial Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e16246 ◽  
Author(s):  
Matthew L. Mayer ◽  
Jared A. Sheridan ◽  
Christoph J. Blohmke ◽  
Stuart E. Turvey ◽  
Robert E. W. Hancock
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Epithelial Cells ◽  
Homoserine Lactone ◽  
Airway Epithelial Cells ◽  
Airway Epithelial ◽  
Cystic Fibrosis Airway

scholarly journals Polymicrobial Interactions in the Cystic Fibrosis Airway Microbiome Impact the Antimicrobial Susceptibility of Pseudomonas aeruginosa

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 827
Author(s):  
Emma Reece ◽  
Pedro H. de Almeida Bettio ◽  
Julie Renwick
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Aeruginosa ◽  
Airway Disease ◽  
Chronic Infections ◽  
Sensitivity Testing ◽  
Antimicrobial Sensitivity ◽  
Cystic Fibrosis Airway ◽  
Airway Microbiome ◽  
Diverse Community

Pseudomonas aeruginosa is one of the most dominant pathogens in cystic fibrosis (CF) airway disease and contributes to significant inflammation, airway damage, and poorer disease outcomes. The CF airway is now known to be host to a complex community of microorganisms, and polymicrobial interactions have been shown to play an important role in shaping P. aeruginosa pathogenicity and resistance. P. aeruginosa can cause chronic infections that once established are almost impossible to eradicate with antibiotics. CF patients that develop chronic P. aeruginosa infection have poorer lung function, higher morbidity, and a reduced life expectancy. P. aeruginosa adapts to the CF airway and quickly develops resistance to several antibiotics. A perplexing phenomenon is the disparity between in vitro antimicrobial sensitivity testing and clinical response. Considering the CF airway is host to a diverse community of microorganisms or ‘microbiome’ and that these microorganisms are known to interact, the antimicrobial resistance and progression of P. aeruginosa infection is likely influenced by these microbial relationships. This review combines the literature to date on interactions between P. aeruginosa and other airway microorganisms and the influence of these interactions on P. aeruginosa tolerance to antimicrobials.

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