Characterization of the Solvent Systems Used in Countercurrent Chromatography

1999 ◽  
pp. 19-46
Keyword(s):  
Countercurrent Chromatography ◽  
Solvent Systems

scholarly journals Isolation Detection and Characterization of Syringolin A produced from the Probiotic Strain Bacillus Cereus Isolated from Donkey Milk

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Rashmi D ◽  
Sharmila T ◽  
Sushama Patil ◽  
Onkar Apine ◽  
Srinivas Sistla ◽  
...  
Keyword(s):  
Probiotic Strain ◽  
Uv Spectra ◽  
Organic Layer ◽  
Characteristic Peak ◽  
Antitumor Compound ◽  
Solvent Systems ◽  
Culture Supernatants ◽  
Layer Chromatography ◽  
Economical Process

Syringolin A is a non-ribosomal virulence factor secreted by few Pseudomonas strains. Syringolin A is an well known irreversible proteasome inhibitor and antitumor compound. The present study is focused on the extraction of Syringolin A through a non-tedious and economical process. Syringolin A is extracted from culture supernatants by the immiscible organic layer by mixing of dichloromethane or chloroform (trichloromethane). Syringolin A was identified by the characteristic peak at 350 nm by UV spectra. The compound was further characterized by Thin Layer Chromatography (TLC) with the retention value, Rf was found to be in the range of 0.78-0.83 run using a combination of solvent systems water and methanol.  The molecular weight of the compound was found to be 492.2614 g mol-1 identified and analyzed by UHPLC–QTOF-MS analysis. Due to its significant pharmacological importance in proliferative diseases, further studies on production and optimization of these compounds are necessary.   

Fabrication and Characterization of Fibrous Polycaprolactone Blended with Natural Green Tea Extracts Using Dual Solvent Systems

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Ainatul Aqilah Binti Mohd Rashid ◽  
Saravana Kumar Jaganathan ◽  
Ahmad Zahran Mohd Khudzari ◽  
Ahmad Fauzi Ismail
Keyword(s):  
Electron Microscopy ◽  
Green Tea ◽  
Water Uptake ◽  
Wound Dressing ◽  
Electrospun Fibers ◽  
Fibrous Morphology ◽  
Solvent Systems ◽  
Fabrication And Characterization ◽  
Scanning Electron

AbstractNanofibrous dressings serve as an impeccable candidate in the management of wounds. Nanofibrous composites composed of polycaprolactone (PCL) and green tea using dual solvent systems at different ratios were fabricated through electrospinning. Pure PCL electrospun fibers along with composites were characterized by using scanning electron microscopy (SEM), wettability, water uptake analysis, and Fourier transform infrared spectroscopy (FTIR). SEM indicated that fibrous morphology and the diameter of PCL/green tea were smaller for chloroform/dimethylformamide (DMF) (601 nm) and acetone/DMF (896 nm) than the pure PCL (673 nm and 1,104 nm for chloroform/DMF and acetone/DMF, respectively). Wettability of the fabricated composites was increased, and pure PCL fibers were slightly more hydrophobic (100°) than PCL/green tea (94°). Water uptake of the composites was enhanced compared with PCL significantly in acetone/DMF. The PCL/green tea nanofibrous wound dressing with enhanced physicochemical properties serves as an indispensable candidate for wound healing applications.

Classification of countercurrent chromatography solvent systems on the basis of the capillary wavelength

1994 ◽  
Vol 66 (1) ◽  
pp. 168-176 ◽  
Author(s):  
J. M. Menet ◽  
D. Thiebaut ◽  
R. Rosset ◽  
J. E. Wesfreid ◽  
M. Martin
Keyword(s):  
Countercurrent Chromatography ◽  
Solvent Systems
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