Genetic and Molecular Characterization of Ca2+ and IP3 Signaling in the Nematode Caenorhabditis elegans

2005 ◽  
pp. 161-186
Author(s):  
Kevin Strange ◽  
Ana Estevez
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Characterization ◽  
Nematode Caenorhabditis Elegans

Molecular characterization of a novel RhoGAP, RRC-1 of the nematode Caenorhabditis elegans

2007 ◽  
Vol 357 (2) ◽  
pp. 377-382 ◽  
Author(s):  
Mina Delawary ◽  
Takanobu Nakazawa ◽  
Tohru Tezuka ◽  
Mariko Sawa ◽  
Yuichi Iino ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Characterization ◽  
Nematode Caenorhabditis Elegans

scholarly journals Molecular characterization of the transition to mid-life in Caenorhabditis elegans

AGE ◽  
2012 ◽  
Vol 35 (3) ◽  
pp. 689-703 ◽  
Author(s):  
D. Mark Eckley ◽  
Salim Rahimi ◽  
Sandra Mantilla ◽  
Nikita V. Orlov ◽  
Christopher E. Coletta ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Characterization

scholarly journals Cloning and biochemical characterization of the cyclophilin homologues from the free-living nematode Caenorhabditis elegans

1996 ◽  
Vol 317 (1) ◽  
pp. 179-185 ◽  
Author(s):  
Antony P. PAGE ◽  
Kenneth MacNIVEN ◽  
Michael O. HENGARTNER
Keyword(s):  
Signal Transduction ◽  
Protein Folding ◽  
Caenorhabditis Elegans ◽  
Biochemical Characterization ◽  
Single Species ◽  
Immunosuppressive Agent ◽  
Specific Substrate ◽  
Nematode Caenorhabditis Elegans ◽  
Isomerase Activity

Cyclosporin A (CsA) is the most widely used immunosuppressive agent, whose properties are exerted via an interaction with cyclophilin, resulting in down-regulation of signal-transduction events in the T-cell. Cyclophilin is identical with peptidylprolyl cis–trans isomerase (PPI; EC 5.2.1.8), an enzyme which catalyses the isomerization between the two proline conformations in proteins, thereby acting as a catalyst in protein-folding events. Several reports indicate that CsA has potent anti-parasitic activity, effective against both protozoan and helminth species. In order to understand the various biological roles that cyclophilins play we have initiated a study of these proteins in the genetically tractable nematode Caenorhabditis elegans. Here we describe the cloning and characterization of 11 cyclophilin genes (cyp-1 to -11) derived from this nematode; this is currently the greatest number of isoforms described in a single species. Southern blotting and physical mapping indicated that these genes are dispersed throughout the nematode genome. A high degree of conservation exists between several isoforms, which also share characteristics with the ubiquitous isoforms previously described. The remaining isoforms are divergent, having altered CsA-binding domains and additional non-cyclophilin domains, which may impart compartmental specificity. Ten of these isoforms have been expressed in Escherichia coli, and the resultant fusion proteins have been examined biochemically for PPI activity, which they all possess. Isomerase activity is highest in the conserved and lowest in divergent isoforms, perhaps indicating a more specific substrate for the latter. Analysis of the C. elegans cyp genes will provide answers as to the roles played by cyclophilins in protein folding and signal transduction.

scholarly journals Isolatio and characterization of metallothionein from nematode(Caenorhabditis elegans)

Eisei kagaku ◽  
1986 ◽  
Vol 32 (1) ◽  
pp. 22-27 ◽  
Author(s):  
KOHJI MARUYAMA ◽  
RITSUKO HORI ◽  
TSUTOMU NISHIHARA ◽  
MASAOMI KONDO
Keyword(s):  
Caenorhabditis Elegans ◽  
Nematode Caenorhabditis Elegans

Viable maternal-effect mutations that affect the development of the nematode Caenorhabditis elegans.

Genetics ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1351-1364 ◽  
Author(s):  
S Hekimi ◽  
P Boutis ◽  
B Lakowski
Keyword(s):  
Quantitative Analysis ◽  
Caenorhabditis Elegans ◽  
Maternal Effect ◽  
Genetic Screen ◽  
Phenotypic Characterization ◽  
Nematode Caenorhabditis Elegans ◽  
Critical Function ◽  
Complementation Groups

Abstract We carried out a genetic screen for viable maternal-effect mutants to identify genes with a critical function relatively early in development. This type of mutation would not have been identified readily in previous screens for viable mutants and therefore could define previously unidentified genes. We screened 30,000 genomes and identified 41 mutations falling into 24 complementation groups. We genetically mapped these 24 loci; only two of them appear to correspond to previously identified genes. We present a partial phenotypic characterization of the mutants and a quantitative analysis of the degree to which they can be maternally or zygotically rescued.

Molecular characterization of the metabotropic glutamate receptor family in Caenorhabditis elegans

2006 ◽  
Vol 34 (5) ◽  
pp. 942-948 ◽  
Author(s):  
J. Dillon ◽  
N.A. Hopper ◽  
L. Holden-Dye ◽  
V. O'Connor
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Characterization ◽  
Metabotropic Glutamate Receptors ◽  
Metabotropic Glutamate Receptor ◽  
Model Organism ◽  
Metabotropic Glutamate ◽  
C Elegans ◽  
Genes Encoding ◽  
G Protein Coupled

mGluRs (metabotropic glutamate receptors) are G-protein-coupled receptors that play an important neuromodulatory role in the brain. Glutamatergic transmission itself plays a fundamental role in the simple nervous system of the model organism Caenorhabditis elegans, but little is known about the contribution made by mGluR signalling. The sequenced genome of C. elegans predicts three distinct genes, mgl-1, mgl-2 and mgl-3 (designated Y4C6A.2). We have used in silico and cDNA analyses to investigate the genes encoding mgls. Our results indicate that mgl genes constitute a gene family made up of three distinct subclasses of receptor. Our transcript analysis highlights potential for complex gene regulation with respect to both expression and splicing. Further, we identify that the predicted proteins encoded by mgls harbour structural motifs that are likely to regulate function. Taken together, this molecular characterization provides a platform to further investigate mGluR function in the model organism C. elegans.

scholarly journals IDENTIFICATION AND CHARACTERIZATION OF 22 GENES THAT AFFECT THE VULVAL CELL LINEAGES OF THE NEMATODE CAENORHABDITIS ELEGANS

Genetics ◽  
1985 ◽  
Vol 110 (1) ◽  
pp. 17-72
Author(s):  
Edwin L Ferguson ◽  
H Robert Horvitz
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Function ◽  
Ventral Side ◽  
Recessive Mutation ◽  
Partial Reduction ◽  
Nematode Caenorhabditis Elegans ◽  
Multiple Alleles ◽  
Cell Lineages ◽  
Identification And Characterization

ABSTRACT Ninety-five mutants of the nematode Caenorhabditis elegans altered in the cell lineages of the vulva have been isolated on the basis of their displaying one of two phenotypes, Vulvaless or Multivulva. In Vulvaless mutants, which define 12 genes, no vulva is present. In Multivulva mutants, which define ten genes, one or more supernumerary vulva-like protrusions are located along the ventral side of the animal. A single recessive mutation is responsible for the phenotypes of most, but not all, of these strains. Fifteen of these 22 genes are represented by multiple alleles. We have shown by a variety of genetic criteria that mutations that result in a Vulvaless or Multivulva phenotype in six of the 22 genes most likely eliminate gene function. In addition, Vulvaless or Multivulva mutations in seven of the other genes most likely result in a partial reduction of gene function; the absence of the activity of any of these genes probably results in lethality or sterility. Our results suggest that we may have identified most, or all, genes of these two classes.

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