Aflatoxin B 1 and Cell Cycle Perturbation

2005 ◽  
Author(s):  
Ruggero Ricordy ◽  
Emanuele Cacci ◽  
Gabriella Augusti-Tocco
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Perturbation ◽  
Aflatoxin B

scholarly journals Exogenous Interleukin-2 Administration Corrects the Cell Cycle Perturbation of Lymphocytes from Human Immunodeficiency Virus-Infected Individuals

2001 ◽  
Vol 75 (22) ◽  
pp. 10843-10855 ◽  
Author(s):  
Mirko Paiardini ◽  
Domenico Galati ◽  
Barbara Cervasi ◽  
Giuseppe Cannavo ◽  
Luca Galluzzi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Human Immunodeficiency Virus ◽  
T Cells ◽  
Cell Cycle Control ◽  
Interleukin 2 ◽  
Protein Ubiquitination ◽  
Immunodeficiency Virus ◽  
Cell Cycle Perturbation ◽  
Cycle Dependent

ABSTRACT Human immunodeficiency virus (HIV)-induced immunodeficiency is characterized by progressive loss of CD4+ T cells associated with functional abnormalities of the surviving lymphocytes. Increased susceptibility to apoptosis and loss of proper cell cycle control can be observed in lymphocytes from HIV-infected individuals and may contribute to the lymphocyte dysfunction of AIDS patients. To better understand the relation between T-cell activation, apoptosis, and cell cycle perturbation, we studied the effect of exogenous interleukin-2 (IL-2) administration on the intracellular turnover of phase-dependent proteins. Circulating T cells from HIV-infected patients display a marked discrepancy between a metabolic profile typical of G0 and a pattern of expression of phase-dependent proteins that indicates a more-advanced position within the cell cycle. This discrepancy is enhanced by in vitro activation with ConA and ultimately results in a marked increase of apoptotic events. Conversely, treatment of lymphocytes with IL-2 alone restores the phase-specific pattern of expression of cell cycle-dependent proteins and is associated with low levels of apoptosis. Interestingly, exogenous IL-2 administration normalizes the overall intracellular protein turnover, as measured by protein synthesis, half-life of newly synthesised proteins, and total protein ubiquitination, thus providing a possible explanation for the effect of IL-2 on the intracellular kinetics of cell cycle-dependent proteins. The beneficial effect of IL-2 administration is consistent with the possibility of defective IL-2 function in vivo, which is confirmed by the observation that lymphocytes from HIV-infected patients show abnormal endogenous IL-2 paracrine/autocrine function upon in vitro mitogen stimulation. Overall these results confirm that perturbation of cell cycle control contributes to HIV-related lymphocyte dysfunction and, by showing that IL-2 administration can revert this perturbation, suggest a new mechanism of action of IL-2 therapy in HIV-infected patients.

Acyclovir induces cell cycle perturbation and apoptosis in Jurkat leukemia cells, and enhances chemotherapeutic drug cytotoxicity

Life Sciences ◽  
2018 ◽  
Vol 215 ◽  
pp. 80-85 ◽  
Author(s):  
Serena Benedetti ◽  
Simona Catalani ◽  
Francesco Palma ◽  
Barbara Canonico ◽  
Francesca Luchetti ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Leukemia Cells ◽  
Chemotherapeutic Drug ◽  
Cell Cycle Perturbation

scholarly journals Induction of DNA damage, apoptosis and cell cycle perturbation mediate cytotoxic activity of new 5-aminosalicylate–4-thiazolinone hybrid derivatives

2020 ◽  
Vol 131 ◽  
pp. 110571 ◽  
Author(s):  
Wafaa S Ramadan ◽  
Ekram M Saleh ◽  
Varsha Menon ◽  
Cijo George Vazhappilly ◽  
Hajjaj H.M. Abdu-Allah ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Dna Damage ◽  
Cytotoxic Activity ◽  
Cell Cycle Perturbation

scholarly journals Development of optogenetic tools to manipulate cell cycle checkpoints

2020 ◽  
Author(s):  
Yuhei Goto ◽  
Kazuhiro Aoki
Keyword(s):  
Cell Cycle ◽  
Spindle Assembly Checkpoint ◽  
Red Light ◽  
Cell Cycle Checkpoints ◽  
Yeast Cells ◽  
M Cell ◽  
Cycle Arrest ◽  
Dependent Protein ◽  
Cell Cycle Perturbation ◽  
Protein Recruitment

SUMMARYIn order to understand the systematic regulation of the cell cycle, we need more precise tools for cell-cycle perturbation. Optogenetics is a powerful technique for precisely controlling cellular signaling at higher spatial and temporal resolution. Here, we report optogenetic tools for the rapid and reversible control of cell-cycle checkpoints with a red/far-red light photoreceptor, phytochrome B (PhyB). We established fission yeast cells producing phycocyanobilin as a chromophore of PhyB, and demonstrated light-dependent protein recruitment to the plasma membrane, nucleus, and kinetochore. Using this system, we developed optogenetic manipulation of the cell cycle in two ways: the Opto-G2/M checkpoint triggered G2/M cell cycle arrest in response to red light, and Opto-SAC induced a spindle assembly checkpoint (SAC) in response to red light and then quickly released the SAC by far-red light.

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