scholarly journals Functional Calcium Binding Peptides from Pacific Cod (Gadus macrocephalus) Bone: Calcium Bioavailability Enhancing Activity and Anti-Osteoporosis Effects in the Ovariectomy-Induced Osteoporosis Rat Model

Nutrients ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 1325 ◽  
Author(s):  
Kai Zhang ◽  
Bafang Li ◽  
Qianru Chen ◽  
Zhaohui Zhang ◽  
Xue Zhao ◽  
...  
Keyword(s):  
Serum Calcium ◽  
Rat Model ◽  
Calcium Binding ◽  
Calcium Absorption ◽  
Intestinal Calcium Absorption ◽  
Pacific Cod ◽  
Pacific Cod Gadus Macrocephalus ◽  
Calcium Bioavailability ◽  
Gadus Macrocephalus ◽  
Binding Peptides

Calcium binding peptides from Pacific cod (Gadus macrocephalus) bone have attracted attention due to their potential effects on bone health. In this study, calcium binding peptides (CBP) were prepared from Pacific cod bone by trypsin and neutral protease. Ultraviolet spectra, circular dichroism (CD), and Fourier transform infrared spectroscopy (FTIR) revealed that carboxyl and amino groups in CBP could bind to Ca2+, and form the peptide-calcium complex (CBP-Ca). Single-pass intestinal perfusion (SPIP) experiments indicated that the intestinal calcium absorption was significantly enhanced (p < 0.01) in CBP-Ca treated Wistar rats. The anti-osteoporosis activity of CBP-Ca was investigated in the ovariectomized (OVX) Wistar rat model. The administration of CBP-Ca significantly (p < 0.01) improved the calcium bioavailability, trabecular bone structure, bone biomechanical properties, bone mineral density, and bone mineralization degree. CBP-Ca notably (p < 0.01) increased serum calcium, however, it remarkably (p < 0.01) reduced the levels of osteocalcin (OCN), bone alkaline phosphatase (BALP), tartrate-resistant acid phosphatase isoform 5b (TRAP5b), and C-telopeptide of type I collagen (CTX-1) in serum. Results suggested that the cod bone derived CBP could bind with calcium, improve the intestinal calcium absorption, calcium bioavailability, and serum calcium, then reduce the bone turnover rate, and thus ameliorate osteoporosis.

High affinity calcium binding peptides from the gills, gut and kidneys of the freshwater eel (Anguilla anguilla)

1978 ◽  
Vol 536 (2) ◽  
pp. 429-432 ◽  
Author(s):  
Peter R. Hearn ◽  
Stephen Tomlinson ◽  
Homa Mellersh ◽  
Christopher J. Kenyon ◽  
Christopher J. Preston ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Anguilla Anguilla ◽  
High Affinity ◽  
Freshwater Eel ◽  
Binding Peptides

scholarly journals 1K1400 Analysis of the interaction between synthetic calcium binding peptides and a metal ion by infrared spectroscopy

2002 ◽  
Vol 42 (supplement2) ◽  
pp. S63
Author(s):  
M. Nara ◽  
H. Morii ◽  
F. Yumoto ◽  
H. Kagi ◽  
M. Tanokura
Keyword(s):  
Infrared Spectroscopy ◽  
Calcium Binding ◽  
Metal Ion ◽  
Binding Peptides

Calcium Binding Peptides from α-Lactalbumin:  Implications for Protein Folding and Stability†

Biochemistry ◽  
1997 ◽  
Vol 36 (15) ◽  
pp. 4607-4615 ◽  
Author(s):  
Brian Kuhlman ◽  
Judith A. Boice ◽  
Wen-Jin Wu ◽  
Robert Fairman ◽  
Daniel P. Raleigh
Keyword(s):  
Protein Folding ◽  
Calcium Binding ◽  
Binding Peptides

ChemInform Abstract: Synthetic Calcium-Binding Peptides

ChemInform ◽  
2010 ◽  
Vol 33 (48) ◽  
pp. no-no
Author(s):  
Gary S. Shaw
Keyword(s):  
Calcium Binding ◽  
Binding Peptides

scholarly journals Isolation of Calcium-Binding Peptides from Barley Protein Hydrolysates

2012 ◽  
Vol 19 (3) ◽  
pp. 438-442 ◽  
Author(s):  
Ji-Hye Lee ◽  
Dong-Won Choi ◽  
Kyung-Bin Song
Keyword(s):  
Calcium Binding ◽  
Protein Hydrolysates ◽  
Binding Peptides ◽  
Barley Protein
Sign in / Sign up

Export Citation Format

Share Document