Maintaining Genetic Diversity in Laboratory Colonies of Parasites and Predators

2018 ◽  
pp. 133-146 ◽  
Author(s):  
Alan C. Bartlett
Keyword(s):  
Genetic Diversity ◽  
Laboratory Colonies

scholarly journals Application of a dense genetic map for assessment of genomic responses to selection and inbreeding in Heliothis virescens.

2015 ◽  
Author(s):  
Megan Fritz ◽  
Sandra Paa ◽  
Jennifer Baltzegar ◽  
Fred Gould
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Heliothis Virescens ◽  
Low Frequency ◽  
Pest Species ◽  
Bt Resistance ◽  
Genomic Analyses ◽  
Laboratory Colonies ◽  
Single Allele ◽  
Small Clusters

Adaptation of pest species to laboratory conditions and selection for resistance to toxins in the laboratory are expected to cause inbreeding and genetic bottlenecks that reduce genetic variation. Heliothis virescens, a major cotton pest, has been colonized in the laboratory many times, and a few laboratory colonies have been selected for Bt resistance. We developed 350 bp Double-Digest Restriction-site Associated DNA-sequencing (ddRAD-seq) molecular markers to examine and compare changes in genetic variation associated with laboratory adaptation, artificial selection, and inbreeding in this non-model insect species. We found that allelic and nucleotide diversity declined dramatically in laboratory-reared H. virescens as compared with field-collected populations. The declines were primarily due to the loss of low frequency alleles present in field-collected H. virescens. A further, albeit modest decline in genetic diversity was observed in a Bt-selected population. The greatest decline was seen in H. virescens that were sib-mated for 10 generations, where more than 80% of loci were fixed for a single allele. To determine which regions of the genome were resistant to fixation in our sib-mated line, we generated a dense intraspecific linkage map containing 3 PCR-based, and 659 ddRAD-seq markers. Markers that retained polymorphism were observed in small clusters spread over multiple linkage groups, but this clustering was not statistically significant. Here, we confirmed and extended the general expectations for reduced genetic diversity in laboratory colonies, provided tools for further genomic analyses, and produced highly homozygous genomic DNA for future whole genome sequencing of H. virescens.

Genetic diversity of Peucedanum japonicum using Internal Transcribed Spacer (ITS) sequence

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YH Kim ◽  
JA Ryuk ◽  
BS Ko ◽  
JW Lee ◽  
SE Oh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Its Sequence

Genetic diversity revealed by ISSR molecular marker in different species of Ocimum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Shinde ◽  
V Shinde ◽  
J Kurane ◽  
A Harsulkar ◽  
K Mahadik
Keyword(s):  
Genetic Diversity ◽  
Molecular Marker

Genetic diversity and phytochemical characterisation of Sideritis scardica populations from Greece

2019 ◽  
Author(s):  
EV Avramidou ◽  
E Sarrou ◽  
P Papaporfiriou ◽  
E Abraham
Keyword(s):  
Genetic Diversity

GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Proteins ◽  
Electrophoretic Analysis ◽  
Protein Characterization ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Phylogenetic Relatedness ◽  
Molecular Weights ◽  
National Centre ◽  
Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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