Metabolic Regulation and Metabolic Engineering for Biofuel and Biochemical Production

2017 ◽  
Author(s):  
Kazuyuki Shimizu
Keyword(s):  
Metabolic Engineering ◽  
Metabolic Regulation ◽  
Biochemical Production

scholarly journals Current Status and Future Strategies to Increase Secondary Metabolite Production from Cyanobacteria

2020 ◽  
Vol 8 (12) ◽  
pp. 1849
Author(s):  
Yujin Jeong ◽  
Sang-Hyeok Cho ◽  
Hookeun Lee ◽  
Hyung-Kyoon Choi ◽  
Dong-Myung Kim ◽  
...  
Keyword(s):  
Metabolic Engineering ◽  
Systems Biology ◽  
Secondary Metabolites ◽  
Regulatory Networks ◽  
Carbon Fixation ◽  
Industrial Applications ◽  
Current Status ◽  
Enhanced Production ◽  
Biochemical Production ◽  
Genome Scale

Cyanobacteria, given their ability to produce various secondary metabolites utilizing solar energy and carbon dioxide, are a potential platform for sustainable production of biochemicals. Until now, conventional metabolic engineering approaches have been applied to various cyanobacterial species for enhanced production of industrially valued compounds, including secondary metabolites and non-natural biochemicals. However, the shortage of understanding of cyanobacterial metabolic and regulatory networks for atmospheric carbon fixation to biochemical production and the lack of available engineering tools limit the potential of cyanobacteria for industrial applications. Recently, to overcome the limitations, synthetic biology tools and systems biology approaches such as genome-scale modeling based on diverse omics data have been applied to cyanobacteria. This review covers the synthetic and systems biology approaches for advanced metabolic engineering of cyanobacteria.

scholarly journals Metabolic Regulation Analysis of Ajmalicine Biosynthesis Pathway in Catharanthus roseus (L.) G. Don Suspension Culture Using Nanosensor

Processes ◽  
2020 ◽  
Vol 8 (5) ◽  
pp. 589
Author(s):  
Ghazala Ambrin ◽  
Hayssam M. Ali ◽  
Altaf Ahmad
Keyword(s):  
Metabolic Engineering ◽  
Cell Lines ◽  
Metabolic Regulation ◽  
Antihypertensive Drugs ◽  
Antioxidant Activities ◽  
Regulatory Gene ◽  
Indole Alkaloid ◽  
Resonance Energy ◽  
Flux Analysis ◽  
Suspension Cells

Ajmalicine is one of the most popular antihypertensive drugs obtained from the root barks of Cathranthus roseus (L.) G. Don and Rauvolfia serpentine (L.) Benth. ex Kurz. It has also potential antimicrobial, cytotoxic, central depressant and antioxidant activities. As the demand for the alkaloid is significantly high, metabolic engineering approaches are being tried to increase its production in both homologous and heterologous systems. The metabolic engineering approach requires knowledge of the metabolic regulation of the alkaloid. For understanding the metabolic regulation, fluxomic analysis is important as it helps in understanding the flux of the alkaloid through the complicated metabolic pathway. The present study was conducted to analyse the flux analysis of the ajmalicine biosynthesis, using a genetically encoded Fluorescent Resonance Energy Transfer FRET-based nanosensor for ajmalicine (FLIP-Ajn). Here, we have silenced six important genes of terpenoid indole alkaloid (TIA), namely G10H, 10HGO, TDC, SLS, STR and SDG, through RNA-mediated gene silencing in different batches of C. roseus suspension cells, generating six silenced cell lines. Monitoring of the ajmalicine level was carried out using FLIP-Ajn in these silenced cell lines, with high spatial and temporal resolution. The study offers the rapid, high throughput real-time measurement of ajmalicine flux in response to the silenced TIA genes, thereby identifying the regulatory gene controlling the alkaloid flux in C. roseus suspension cells. We have reported that the STR gene encoding strictosidine synthase of the TIA pathway could be the regulatory gene of the ajmalicine biosynthesis.

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