A simulated-use evaluation of a strategy for preventing biofilm formation in dental unit waterlines*

2009 ◽  
pp. 91-102
Keyword(s):  
Biofilm Formation ◽  
Dental Unit Waterlines

Biofilm formation and control in dental unit waterlines

Biofilms ◽  
2005 ◽  
Vol 2 (1) ◽  
pp. 9-17 ◽  
Author(s):  
F. F. S. Franco ◽  
D. Spratt ◽  
J. C. Leao ◽  
S. R. Porter
Keyword(s):  
Public Health ◽  
Health Risk ◽  
Current Literature ◽  
Biofilm Formation ◽  
Microbial Control ◽  
Dental Practice ◽  
Public Health Risk ◽  
Published Evidence ◽  
Dental Unit Waterlines ◽  
And Control

This review details the current literature on relevant aspects of biofilm formation and microbial control in dental practice. To date, there is no published evidence of a serious public health risk from biofilm-contaminated dental waterlines. However, there remain few effective methods of decontamination of such waterlines.

Inhibitory effect of PVDF tubes on biofilm formation in dental unit waterlines

2005 ◽  
Vol 21 (8) ◽  
pp. 780-786 ◽  
Author(s):  
Toshiaki Yabune ◽  
Satoshi Imazato ◽  
Shigeyuki Ebisu
Keyword(s):  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Dental Unit Waterlines

scholarly journals Substantivity of hypochlorous acid-based disinfectant against biofilm formation in the dental unit waterlines

2017 ◽  
Vol 20 (1) ◽  
pp. 2 ◽  
Author(s):  
IrfanaFathima Shajahan ◽  
Deivanayagam Kandaswamy ◽  
L Lakshminarayanan ◽  
R Selvarajan
Keyword(s):  
Biofilm Formation ◽  
Hypochlorous Acid ◽  
Dental Unit Waterlines

scholarly journals Assessment of Inhibitory Effects of Fluoride-Coated Tubes on Biofilm Formation by Using the In Vitro Dental Unit Waterline Biofilm Model

2008 ◽  
Vol 74 (19) ◽  
pp. 5958-5964 ◽  
Author(s):  
Toshiaki Yabune ◽  
Satoshi Imazato ◽  
Shigeyuki Ebisu
Keyword(s):  
Biofilm Formation ◽  
Polyvinylidene Fluoride ◽  
In Vitro Model ◽  
Bacterial Species ◽  
Biofilm Model ◽  
Dental Unit Waterlines ◽  
Vitro Model ◽  
Methylobacterium Mesophilicum ◽  
The One

ABSTRACT This study aimed to establish an in vitro model to simulate biofilms formed in dental unit waterlines (DUWLs) and to investigate the ability of polyvinylidene fluoride (PVDF)-coated tubes to inhibit biofilm formation using this model. The water and biofilm samples were obtained from DUWLs which had been clinically used for 2.5 years, and the predominant bacteria were identified. A conventional polyurethane tube was incubated for 24 to 96 h in the mixed flora of isolated bacteria, and the optimal incubation conditions to simulate a clinically formed biofilm were determined by observation with a scanning electron microscope. Biofilm formation on a PVDF-coated tube was observed using this in vitro model, and the adherence of different bacterial species to conventional and PVDF-coated tubes was assessed. Sphingomonas paucimobilis, Acinetobacter haemolytics, and Methylobacterium mesophilicum were predominantly isolated from contaminated DUWLs. Incubation of the polyurethane tube with the mixed flora containing these three species for 96 h resulted in the formation of a mature biofilm similar to the one clinically observed. The PVDF-coated tube was significantly less adhesive to all three bacterial species than the polyurethane tube (P < 0.05 by the Mann-Whitney U test), and the attachment of small amounts of rods was observed even after incubation with the mixed flora for 96 h. In conclusion, an in vitro biofilm model was obtained by using a mixed flora of bacteria isolated from DUWLs, and the PVDF-coated tube was found to be effective in preventing biofilm formation using this model.

Bacterial biofilm behavior in water-supply lines of dental units

1992 ◽  
Vol 50 (1) ◽  
pp. 882-883
Author(s):  
B.D. Tall ◽  
K.S. George ◽  
R. T. Gray ◽  
H.N. Williams
Keyword(s):  
Water Supply ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Bacterial Biofilm

Studies of bacterial behavior in many environments have shown that most organisms attach to surfaces, forming communities of microcolonies called biofilms. In contaminated medical devices, biofilms may serve both as reservoirs and as inocula for the initiation of infections. Recently, there has been much concern about the potential of dental units to transmit infections. Because the mechanisms of biofilm formation are ill-defined, we investigated the behavior and formation of a biofilm associated with tubing leading to the water syringe of a dental unit over a period of 1 month.

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