scholarly journals Characterization and relative quantification of phospholipids based on methylation and stable isotopic labeling

2016 ◽  
Vol 57 (3) ◽  
pp. 388-397 ◽  
Author(s):  
Tanxi Cai ◽  
Qingbo Shu ◽  
Peibin Liu ◽  
Lili Niu ◽  
Xiaojing Guo ◽  
...  
Keyword(s):  
Isotopic Labeling ◽  
Relative Quantification ◽  
Stable Isotopic

scholarly journals Comparison of LFQ and IPTL for Protein Identification and Relative Quantification

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5330
Author(s):  
Christina Johannsen ◽  
Christian J. Koehler ◽  
Bernd Thiede
Keyword(s):  
Protein Identification ◽  
Isotopic Labeling ◽  
Metabolic Labeling ◽  
Relative Quantification ◽  
Label Free ◽  
Label Free Quantification ◽  
Stable Isotopic ◽  
Quantification Accuracy ◽  
The Many ◽  
Free Quantification

(1) Background: Mass spectrometry-based quantitative proteome profiling is most commonly performed by label-free quantification (LFQ), stable isotopic labeling with amino acids in cell culture (SILAC), and reporter ion-based isobaric labeling methods (TMT and iTRAQ). Isobaric peptide termini labeling (IPTL) was described as an alternative to these methods and is based on crosswise labeling of both peptide termini and MS2 quantification. High quantification accuracy was assumed for IPTL because multiple quantification points are obtained per identified MS2 spectrum. A direct comparison of IPTL with other quantification methods has not been performed yet because IPTL commonly requires digestion with endoproteinase Lys-C. (2) Methods: To enable tryptic digestion of IPTL samples, a novel labeling for IPTL was developed that combines metabolic labeling (Arg-0/Lys-0 and Arg-d4/Lys-d4, respectively) with crosswise N-terminal dimethylation (d4 and d0, respectively). (3) Results: The comparison of IPTL with LFQ revealed significantly more protein identifications for LFQ above homology ion scores but not above identity ion scores. (4) Conclusions: The quantification accuracy was superior for LFQ despite the many quantification points obtained with IPTL.

Quantification of protein deposits on silicone hydrogel materials using stable-isotopic labeling and multiple reaction monitoring

Biofouling ◽  
2012 ◽  
Vol 28 (7) ◽  
pp. 697-709 ◽  
Author(s):  
Negar Babaei Omali ◽  
Zhenjun Zhao ◽  
Ling Zhong ◽  
Mark J. Raftery ◽  
Hua Zhu ◽  
...  
Keyword(s):  
Multiple Reaction Monitoring ◽  
Isotopic Labeling ◽  
Reaction Monitoring ◽  
Silicone Hydrogel ◽  
Protein Deposits ◽  
Stable Isotopic

Stable Isotopic Labeling for Proteomics

2011 ◽  
pp. 549-573 ◽  
Author(s):  
Keith Ashman ◽  
María Isabel Ruppen Cañás ◽  
Jose L. Luque-Garcia ◽  
Fernando García Martínez
Keyword(s):  
Isotopic Labeling ◽  
Stable Isotopic

scholarly journals Untargeted Profiling of Tracer-Derived Metabolites Using Stable Isotopic Labeling and Fast Polarity-Switching LC–ESI-HRMS

2014 ◽  
Vol 86 (23) ◽  
pp. 11533-11537 ◽  
Author(s):  
Bernhard Kluger ◽  
Christoph Bueschl ◽  
Nora Neumann ◽  
Romana Stückler ◽  
Maria Doppler ◽  
...  
Keyword(s):  
Isotopic Labeling ◽  
Polarity Switching ◽  
Stable Isotopic ◽  
Untargeted Profiling

Stable Isotopic Labeling and Nontarget Identification of Nanogram/Liter Amino Contaminants in Water

2019 ◽  
Vol 91 (20) ◽  
pp. 13213-13221 ◽  
Author(s):  
Zhongshan Liu ◽  
Caley B. Craven ◽  
Guang Huang ◽  
Ping Jiang ◽  
Di Wu ◽  
...  
Keyword(s):  
Isotopic Labeling ◽  
Stable Isotopic
Sign in / Sign up

Export Citation Format

Share Document