scholarly journals 10E,12Z-conjugated linoleic acid impairs adipocyte triglyceride storage by enhancing fatty acid oxidation, lipolysis, and mitochondrial reactive oxygen species

2013 ◽  
Vol 54 (11) ◽  
pp. 2964-2978 ◽  
Author(s):  
Laura J. den Hartigh ◽  
Chang Yeop Han ◽  
Shari Wang ◽  
Mohamed Omer ◽  
Alan Chait
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Fatty Acid Oxidation ◽  
Reactive Oxygen ◽  
Acid Oxidation ◽  
Oxygen Species ◽  
Mitochondrial Reactive Oxygen Species

scholarly journals Lineage-Selective Disturbance of Early Human Hematopoietic Progenitor Cell Differentiation by the Commonly Used Plasticizer Di-2-ethylhexyl Phthalate via Reactive Oxygen Species: Fatty Acid Oxidation Makes the Difference

Cells ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2703
Author(s):  
Lars Kaiser ◽  
Isabel Quint ◽  
René Csuk ◽  
Manfred Jung ◽  
Hans-Peter Deigner
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Energy Production ◽  
Hematopoietic Cells ◽  
Reactive Oxygen ◽  
Health Concern ◽  
Acid Oxidation ◽  
Oxygen Species ◽  
Ethylhexyl Phthalate

Exposure to ubiquitous endocrine-disrupting chemicals (EDCs) is a major public health concern. We analyzed the physiological impact of the EDC, di-2-ethylhexyl phthalate (DEHP), and found that its metabolite, mono-2-ethylhexyl phthalate (MEHP), had significant adverse effects on myeloid hematopoiesis at environmentally relevant concentrations. An analysis of the underlying mechanism revealed that MEHP promotes increases in reactive oxygen species (ROS) by reducing the activity of superoxide dismutase in all lineages, possibly via its actions at the aryl hydrocarbon receptor. This leads to a metabolic shift away from glycolysis toward the pentose phosphate pathway and ultimately results in the death of hematopoietic cells that rely on glycolysis for energy production. By contrast, cells that utilize fatty acid oxidation for energy production are not susceptible to this outcome due to their capacity to uncouple ATP production. These responses were also detected in non-hematopoietic cells exposed to alternate inducers of ROS.

scholarly journals Active fatty acid oxidation defines the cellular response towards reactive oxygen species

2020 ◽  
Author(s):  
Lars Kaiser ◽  
Isabel Quint ◽  
René Csuk ◽  
Manfred Jung ◽  
Hans-Peter Deigner
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acid ◽  
Fatty Acid Oxidation ◽  
Cellular Response ◽  
Reactive Oxygen ◽  
Endocrine Disrupting Compounds ◽  
Acid Oxidation ◽  
Oxygen Species ◽  
Atp Generation ◽  
The Impact

AbstractEndocrine disrupting compounds (EDC) are ubiquitous in the human environment, displaying a highly relevant research topic. The impact of EDC on the differentiation of primitive cells, e.g. in hematopoiesis, is of particular interest. We found profound inhibitory effects of di-2-ethylhexyl phthalate (DEHP) on erythropoiesis and dendropoiesis, mediated via reactive oxygen species (ROS) generation. Neutrophil differentiation, however, was not affected by DEHP. ROS leads to a shift from glycolysis to the pentose phosphate pathway and diminishes ATP generation from glycolysis, ultimately resulting in apoptosis in both cell types. In neutrophils, ATP generation is held constant by active fatty acid oxidation (FAO), rendering these cells highly resistant against ROS. This relationship also holds true in HUVEC and HepG2 cells, also in combination with other organic peroxides. We, therefore, uncover a key mechanism for ROS quenching which further explains the distinct ROS quenching ability of different tissues.

scholarly journals Dietary trans 10, cis 12-conjugated linoleic acid reduces the expression of fatty acid oxidation and drug detoxification enzymes in mouse liver

2007 ◽  
Vol 97 (1) ◽  
pp. 58-66 ◽  
Author(s):  
Reuven Rasooly ◽  
Darshan S. Kelley ◽  
Jeff Greg ◽  
Bruce E. Mackey
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Fatty Liver ◽  
Conjugated Linoleic Acid ◽  
Fatty Acid Oxidation ◽  
Fatty Acid Synthase ◽  
Detoxification Enzymes ◽  
Acid Oxidation ◽  
Drug Detoxification ◽  
Hepatic Genes

Mice fed diets containing trans 10, cis 12 (t10, c12)-conjugated linoleic acid (CLA) develop fatty livers and the role of hepatic fatty acid oxidation enzymes in this development is not well defined. We examined the effects of dietary cis 9, trans 11-CLA (c9, t11-CLA) and t10, c12-CLA on the expression of hepatic genes for fatty acid metabolism. Female mice, 8 weeks old, (six animals per group) were fed either a control diet or diets supplemented with 0·5 % c9, t11- or t10, c12-CLA for 8 weeks. DNA microarray analysis showed that t10, c12-CLA increased the expression of 278 hepatic genes and decreased those of 121 genes (>2-fold); c9, t11-CLA increased expression of twenty-two genes and decreased those of nine. Real-time PCR confirmed that t10, c12-CLA reduced by the expression of fatty acid oxidation genes including flavin monooxygenase (FMO)-3 95 %, cytochrome P450 (cyt P450) 69 %, carnitine palmitoyl transferase 1a 77 %, acetyl CoA oxidase (ACOX) 50 % and PPARα 65 %; it increased the expression of fatty acid synthase by 3·5-fold (P < 0·05 for all genes, except ACOX P = 0·08). It also reduced the enzymatic activity of hepatic microsomal FMO by 40 % and the FMO3 specific protein by 67 %. c9, t11-CLA reduced FMO3 and cyt P450 expression by 61 % (P = 0·001) and 38 % (P = 0·06) and increased steoryl CoA desaturase transcription by 5·9-fold (P = 0·07). Both decreased fatty acid oxidation and increased fatty acid synthesis seem to contribute to the CLA-induced fatty liver. Since FMO and cyt P450 are also involved in drug detoxification, suppression of the transcription of these genes by CLA may have other health consequences besides development of fatty liver.

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