scholarly journals Peripheral blood CD4+ T lymphocytes from multiple sclerosis patients are characterized by higher PSGL-1 expression and transmigration capacity across a human blood-brain barrier-derived endothelial cell line

2009 ◽  
Vol 86 (5) ◽  
pp. 1049-1063 ◽  
Author(s):  
Bouchaib Bahbouhi ◽  
Laureline Berthelot ◽  
Ségolène Pettré ◽  
Laure Michel ◽  
Sandrine Wiertlewski ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Endothelial Cell ◽  
T Lymphocytes ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Peripheral Blood ◽  
Brain Barrier ◽  
Endothelial Cell Line ◽  
Multiple Sclerosis Patients

Development of immortalized human cerebromicrovascular endothelial cell line as an in vitro model of the human blood–brain barrier

1997 ◽  
Vol 11 (13) ◽  
pp. 1187-1197 ◽  
Author(s):  
Arumugam Muruganandam ◽  
Leonie Moorhouse Herx ◽  
Robert Monette ◽  
Jon P. Durkin ◽  
Danica B. Stanimirovic
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
In Vitro Model ◽  
Brain Barrier ◽  
Endothelial Cell Line ◽  
Blood Brain ◽  
Vitro Model

scholarly journals Purine receptors and Ca2+ signalling in the human blood–brain barrier endothelial cell line hCMEC/D3

2011 ◽  
Vol 8 (1) ◽  
pp. 71-80 ◽  
Author(s):  
Willem Bintig ◽  
Daniela Begandt ◽  
Barbara Schlingmann ◽  
Linda Gerhard ◽  
Maria Pangalos ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Human Blood ◽  
Brain Barrier ◽  
Purine Receptors ◽  
Endothelial Cell Line ◽  
Blood Brain

Induction of Blood-Brain Barrier Differentiation in a Rat Brain-Derived Endothelial Cell Line

1995 ◽  
Vol 220 (1) ◽  
pp. 161-170 ◽  
Author(s):  
Delphine Lechardeur ◽  
Bertrand Schwartz ◽  
Denise Paulin ◽  
Daniel Scherman
Keyword(s):  
Endothelial Cell ◽  
Rat Brain ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Endothelial Cell Line ◽  
Blood Brain

scholarly journals Blood‐brain barrier‐specific properties of a human adult brain endothelial cell line

2005 ◽  
Vol 19 (13) ◽  
pp. 1872-1874 ◽  
Author(s):  
B. B. Weksler ◽  
E. A. Subileau ◽  
N. Perrière ◽  
P. Charneau ◽  
K. Holloway ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Adult Brain ◽  
Brain Barrier ◽  
Brain Endothelial Cell ◽  
Endothelial Cell Line ◽  
Human Adult ◽  
Blood Brain

scholarly journals A Proton-Coupled Transport System for β-Hydroxy-β-Methylbutyrate (HMB) in Blood–Brain Barrier Endothelial Cell Line hCMEC/D3

Nutrients ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 3220
Author(s):  
Kei Higuchi ◽  
Sathish Sivaprakasam ◽  
Souad R. Sennoune ◽  
Jiro Ogura ◽  
Yangzom D. Bhutia ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Amino Acid ◽  
Gene Family ◽  
Cell Line ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Monocarboxylate Transporter ◽  
Endothelial Cell Line ◽  
Solute Carrier ◽  
The Brain

β-Hydroxy-β-methylbutyrate (HMB), a leucine metabolite, is used as a nutritional ingredient to improve skeletal muscle health. Preclinical studies indicate that this supplement also elicits significant benefits in the brain; it promotes neurite outgrowth and prevents age-related reductions in neuronal dendrites and cognitive performance. As orally administered HMB elicits these effects in the brain, we infer that HMB crosses the blood–brain barrier (BBB). However, there have been no reports detailing the transport mechanism for HMB in BBB. Here we show that HMB is taken up in the human BBB endothelial cell line hCMEC/D3 via H+-coupled monocarboxylate transporters that also transport lactate and β-hydroxybutyrate. MCT1 (monocarboxylate transporter 1) and MCT4 (monocarboxylate transporter 4) belonging to the solute carrier gene family SLC16 (solute carrier, gene family 16) are involved, but additional transporters also contribute to the process. HMB uptake in BBB endothelial cells results in intracellular acidification, demonstrating cotransport with H+. Since HMB is known to activate mTOR with potential to elicit transcriptomic changes, we examined the influence of HMB on the expression of selective transporters. We found no change in MCT1 and MCT4 expression. Interestingly, the expression of LAT1 (system L amino acid transporter 1), a high-affinity transporter for branched-chain amino acids relevant to neurological disorders such as autism, is induced. This effect is dependent on mTOR (mechanistic target of rapamycine) activation by HMB with no involvement of histone deacetylases. These studies show that HMB in systemic circulation can cross the BBB via carrier-mediated processes, and that it also has a positive influence on the expression of LAT1, an important amino acid transporter in the BBB.

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