scholarly journals Slc11a1-mediated resistance toSalmonella entericaserovar Typhimurium andLeishmania donovaniinfections does not require functional inducible nitric oxide synthase or phagocyte oxidase activity

2004 ◽  
Vol 77 (3) ◽  
pp. 311-320 ◽  
Author(s):  
Jacqueline K. White ◽  
Pietro Mastroeni ◽  
Jean-François Popoff ◽  
Carlton A. W. Evans ◽  
Jenefer M. Blackwell
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxidase Activity ◽  
Phagocyte Oxidase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Phenotype of Mice and Macrophages Deficient in Both Phagocyte Oxidase and Inducible Nitric Oxide Synthase

Immunity ◽  
1999 ◽  
Vol 10 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Michael U Shiloh ◽  
John D MacMicking ◽  
Susan Nicholson ◽  
Juliet E Brause ◽  
Strite Potter ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Phagocyte Oxidase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Granulocytic Ehrlichiosis in Mice Deficient in Phagocyte Oxidase or Inducible Nitric Oxide Synthase

2000 ◽  
Vol 68 (7) ◽  
pp. 4361-4362 ◽  
Author(s):  
Rila Banerjee ◽  
Juan Anguita ◽  
Erol Fikrig
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Reactive Nitrogen ◽  
Granulocytic Ehrlichiosis ◽  
Human Granulocytic Ehrlichiosis ◽  
Reactive Nitrogen Intermediates ◽  
Phagocyte Oxidase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

ABSTRACT Mice deficient in phox (gp91phox−/−) or NOS2 (NOS2−/−) were infected with the agent of human granulocytic ehrlichiosis (HGE) to evaluate the importance of these pathways in the eradication of HGE bacteria. NOS2−/− mice had delayed clearance of the HGE agent in comparison to control or gp91phox−/− mice, suggesting that reactive nitrogen intermediates play a role in the early control of HGE.

scholarly journals Antimicrobial Actions of the Nadph Phagocyte Oxidase and Inducible Nitric Oxide Synthase in Experimental Salmonellosis. II. Effects on Microbial Proliferation and Host Survival in Vivo

2000 ◽  
Vol 192 (2) ◽  
pp. 237-248 ◽  
Author(s):  
Pietro Mastroeni ◽  
Andrés Vazquez-Torres ◽  
Ferric C. Fang ◽  
Yisheng Xu ◽  
Shahid Khan ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Host Resistance ◽  
Wild Type ◽  
Phagocyte Oxidase ◽  
Microbial Proliferation ◽  
Bacterial Replication ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

The roles of the NADPH phagocyte oxidase (phox) and inducible nitric oxide synthase (iNOS) in host resistance to virulent Salmonella typhimurium were investigated in gp91phox−/−, iNOS−/−, and congenic wild-type mice. Although both gp91phox−/− and iNOS−/− mice demonstrated increased susceptibility to infection with S. typhimurium compared with wild-type mice, the kinetics of bacterial replication were dramatically different in the gp91phox−/− and iNOS−/− mouse strains. Greater bacterial numbers were present in the spleens and livers of gp91phox−/− mice compared with C57BL/6 controls as early as day 1 of infection, and all of the gp91phox−/− mice succumbed to infection within 5 d. In contrast, an increased bacterial burden was detected within reticuloendothelial organs of iNOS−/− mice only beyond the first week of infection. Influx of inflammatory CD11b+ cells, granuloma formation, and serum interferon γ levels were unimpaired in iNOS−/− mice, but the iNOS-deficient granulomas were unable to limit bacterial replication. The NADPH phagocye oxidase and iNOS are both required for host resistance to wild-type Salmonella, but appear to operate principally at different stages of infection.

scholarly journals Inducible Nitric Oxide Synthase Regulates Production of Isoprostanes In Vivo during Chlamydial Genital Infection in Mice

2003 ◽  
Vol 71 (12) ◽  
pp. 7183-7187 ◽  
Author(s):  
K. H. Ramsey ◽  
I. M. Sigar ◽  
S. V. Rana ◽  
J. Gupta ◽  
S. M. Holland ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Oxidant Stress ◽  
Genital Infection ◽  
Phagocyte Oxidase ◽  
Nitrogen Radicals ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

ABSTRACT Urinary nitrite and F2-isoprostanes, an index of oxidant stress, were elevated during chlamydial genital infection of mice. Enhancement of urinary nitrite and F2-isoprostanes was observed in phagocyte oxidase-deficient mice. Inhibition of inducible nitric oxide synthase reduced isoprostane excretion. We conclude that nitrogen radicals induce F2-isoprostane production and excretion during murine chlamydial genital infection.

scholarly journals Role for Inducible Nitric Oxide Synthase in Protection from Chronic Chlamydia trachomatis Urogenital Disease in Mice and Its Regulation by Oxygen Free Radicals

2001 ◽  
Vol 69 (12) ◽  
pp. 7374-7379 ◽  
Author(s):  
K. H. Ramsey ◽  
I. M. Sigar ◽  
S. V. Rana ◽  
J. Gupta ◽  
S. M. Holland ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Chlamydia Trachomatis ◽  
Inducible Nitric Oxide Synthase ◽  
Gene Knockout ◽  
Significant Proportion ◽  
Phagocyte Oxidase ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

ABSTRACT It has been previously reported that although inducible nitric oxide synthase (iNOS) gene knockout (NOS2 −/−) mice resolve Chlamydia trachomatis genital infection, the production of reactive nitrogen species (RNS) via iNOS protects a significant proportion of mice from hydrosalpinx formation and infertility. We now report that higher in vivo RNS production correlates with mouse strain-related innate resistance to hydrosalpinx formation. We also show that mice with a deletion of a key component of phagocyte NADPH oxidase (p47 phox−/−) resolve infection, produce greater amounts of RNS in vivo, and sustain lower rates of hydrosalpinx formation than both wild-type (WT)NOS2 +/+ andNOS2 −/− controls. When we induced an in vivo chemical block in iNOS activity in p47 phox−/− mice usingN G-monomethyl-l-arginine (L-NMMA), a large proportion of these mice eventually succumbed to opportunistic infections, but not before they resolved their chlamydial infections. Interestingly, when compared to WT and untreated p47 phox−/− controls, L-NMMA-treated p47 phox−/− mice resolved their infections more rapidly. However, L-NMMA-treated p47 phox−/− mice lost resistance to chronic chlamydial disease, as evidenced by an increased rate of hydrosalpinx formation that was comparable to that forNOS2 −/− mice. We conclude that phagocyte oxidase-derived reactive oxygen species (ROS) regulate RNS during chlamydial urogenital infection in the mouse. We further conclude that while neither phagocyte oxidase-derived ROS nor iNOS-derived RNS are essential for resolution of infection, RNS protect from chronic chlamydial disease in this model.

289: Roles of Inducible Nitric Oxide Synthase in Voiding Function and Bladder Pain During Experimental Cystitis

2006 ◽  
Vol 175 (4S) ◽  
pp. 96-96
Author(s):  
Masayoshi Nomura ◽  
Hisae Nishii ◽  
Masato Tsutsui ◽  
Naohiro Fujimoto ◽  
Tetsuro Matsumoto
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Voiding Function ◽  
Bladder Pain ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide
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