Plasmodium falciparum-free merozoites and infected RBCs distinctly affect soluble CD40 ligand-mediated maturation of immature monocyte-derived dendritic cells

2008 ◽  
Vol 84 (1) ◽  
pp. 244-254 ◽  
Author(s):  
Paushali Mukherjee ◽  
Virander Singh Chauhan
Keyword(s):  
Plasmodium Falciparum ◽  
Dendritic Cells ◽  
Cd40 Ligand ◽  
Soluble Cd40 Ligand ◽  
Infected Rbcs

scholarly journals CD40 up-regulation on dendritic cells correlates with Th17/Treg imbalance in chronic periodontitis in young population

2020 ◽  
Vol 26 (6) ◽  
pp. 482-489
Author(s):  
Xin Su ◽  
Jiahui Zhang ◽  
Xue Qin
Keyword(s):  
Dendritic Cells ◽  
Chronic Periodontitis ◽  
Th17 Cell ◽  
Young Patients ◽  
Serum Levels ◽  
Cd40 Ligand ◽  
Young Population ◽  
Soluble Cd40 Ligand ◽  
Old Patients

We aimed to discover the influence of age on the development of chronic periodontitis and illustrate the molecular mechanism in this process. Blood samples were collected from 63 chronic periodontitis patients and 30 healthy controls. Th17 cell/Foxp3+ regulatory T cell (Treg) ratio and expression of costimulatory molecules in dendritic cells (DCs) were analyzed by flow cytometry. The serum levels of soluble CD40 ligand (CD40L) and IL-17 were examined by ELISA. In young chronic periodontitis patients, the Th17/Treg ratio was significantly higher than that in old patients. CD40 on DCs and serum levels of CD40L and IL-17 were all higher in young chronic periodontitis patients. Mature DCs with high CD40 expression level elevated the Th17/Treg ratio in vitro. During the pathogenesis of chronic periodontitis, young patients had higher Th17/Treg ratio than old patients and this phenomenon was in line with the differential expression levels of CD40 in DCs.

Production of canine soluble CD40 ligand to induce maturation of monocyte derived dendritic cells for cancer immunotherapy

2013 ◽  
Vol 156 (1-2) ◽  
pp. 121-127 ◽  
Author(s):  
Viskam Wijewardana ◽  
Kikuya Sugiura ◽  
Mana Yahata ◽  
Takashi Akazawa ◽  
Daluthgamage Patsy H. Wijesekera ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Cancer Immunotherapy ◽  
Cd40 Ligand ◽  
Soluble Cd40 Ligand

scholarly journals Prostaglandin E2 is a key factor for CCR7 surface expression and migration of monocyte-derived dendritic cells

Blood ◽  
2002 ◽  
Vol 100 (4) ◽  
pp. 1354-1361 ◽  
Author(s):  
Elke Scandella ◽  
Ying Men ◽  
Silke Gillessen ◽  
Reinhold Förster ◽  
Marcus Groettrup
Keyword(s):  
Dendritic Cells ◽  
Lymph Node ◽  
T Cell ◽  
Prostaglandin E2 ◽  
Cyclic Adenosine Monophosphate ◽  
Human Monocyte ◽  
Adenosine Monophosphate ◽  
Cd40 Ligand ◽  
Soluble Cd40 Ligand ◽  
Peripheral Tissues

Dendritic cells (DCs) are potent antigen-presenting cells that are able to initiate and modulate immune responses and are hence exploited as cellular vaccines for immunotherapy. Their capacity to migrate from peripheral tissues to the T-cell areas of draining lymph nodes is crucial for the priming of T lymphocytes. In this study, we investigated how the maturation of human monocyte-derived DCs (MoDCs) by several different stimuli under serum-free conditions affected their T-cell stimulatory function, cytokine secretion, and migratory behavior. Surprisingly, we found that for all maturation stimuli tested, the addition of prostaglandin E2 (PGE2) was required for effective migration of MoDCs toward the lymph node–derived chemokines CCL19 (EBI1 ligand chemokine/macrophage inflammatory protein–-3β) and CCL21 (secondary lymphoid tissue chemokine [SLC]/6Ckine). Costimulation with PGE2 enhanced the expression of the CCL19/CCL21 receptor CCR7 on the cell surface of MoDCs when they were matured with soluble CD40 ligand or proinflammatory cytokines, but did not affect CCR7 expression of polyI:C–stimulated MoDCs. The effects of PGE2 on MoDCs were mediated through increased cyclic adenosine monophosphate by 2 of the known PGE2 receptors, EP2 and EP4, which are expressed and down-regulated after PGE2 binding in these cells. In conclusion, our results suggest that signals provided by the proinflammatory mediator PGE2 are crucial for MoDCs to acquire potent T-helper cell stimulatory capacity and substantial chemotactic responsiveness to lymph node–derived chemokines. This is a new and important parameter for the preparation of MoDCs as cellular vaccines in tumor immunotherapy.

scholarly journals Persistently higher serum sCD40L levels are associated with outcome in septic patients

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Yingjian Liang ◽  
Chengrui Zhu ◽  
Yini Sun ◽  
Zhiliang Li ◽  
Liang Wang ◽  
...  
Keyword(s):  
Intensive Care Unit ◽  
Septic Shock ◽  
Odds Ratio ◽  
Cd40 Ligand ◽  
Surgical Patients ◽  
Soluble Cd40 Ligand ◽  
Icu Admission ◽  
Time Changes ◽  
And Control ◽  
Type Of Surgery

Abstract Background Soluble CD40 ligand (sCD40L) exhibits proinflammatory and procoagulant effects. Recent data indicated that sCD40L plays a significant role in septic patients. The aim of the present study was to determine sCD40L changes in surgical patients without sepsis (SWS) and surgical sepsis patients (SS) during the first 3 days after intensive care unit (ICU) admission and to observe the association between sCD40L and mortality. Methods Time changes in sCD40L levels were assessed for 3 days after ICU admission in 49 patients with SS and compared with those in 19 SWS patients. Serum sCD40L concentration was detected by ELISA. Survival at 28 days served as the endpoint. Results SS had significantly higher sCD40L levels than SWS and control patients. We observed an association between sCD40L levels ≥1028.75 pg/mL at day 2 and 28-day mortality (odds ratio = 7.888; 95% confidence interval = 1.758 to 35.395; P = 0.007). We could not discover any significant differences in sex, presence of septic shock, site of infection, length of stay in the ICU, PaO2/FiO2 ratio, incidence of AKI, ARDS, or type of surgery between nonsurvivors and survivors. Conclusions Septic patients show persistently higher circulating sCD40L levels in the first 3 days after ICU admission, and serum sCD40L levels are associated with the mortality of patients with sepsis. Thus, serum sCD40L may be used as a reliable biomarker and therapeutic target in sepsis.

Sign in / Sign up

Export Citation Format

Share Document