Tonic protein kinase A activity maintains inactive  2 integrins in unstimulated neutrophils by reducing myosin light-chain phosphorylation: role of myosin light-chain kinase and Rho kinase

2008 ◽  
Vol 83 (4) ◽  
pp. 964-971 ◽  
Author(s):  
C. D. Chilcoat ◽  
Y. Sharief ◽  
S. L. Jones
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Rho Kinase ◽  
Myosin Light Chain Phosphorylation ◽  
Kinase A

Effect of Myosin Light Chain Kinase, Protein Kinase A, and Protein Kinase C Inhibition on Porcine Oocyte Activation1

1999 ◽  
Vol 61 (1) ◽  
pp. 111-119 ◽  
Author(s):  
Kristin M. Green ◽  
Jae Hwan Kim ◽  
Wei-Hua Wang ◽  
Billy N. Day ◽  
Randall S. Prather
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Protein Kinase A ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Kinase C ◽  
Porcine Oocyte ◽  
Kinase A

373 Role of myosin light chain kinase and Rho kinase in carbachol-induced contractions of human detrusor smooth muscle

2012 ◽  
Vol 11 (1) ◽  
pp. e373
Author(s):  
C. Protzel ◽  
T. Kirschstein ◽  
K. Porath ◽  
T. Sellmann ◽  
R. Koehling ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Rho Kinase ◽  
Detrusor Smooth Muscle ◽  
Human Detrusor

Shrinkage-induced activation of Na+/H+ exchange in primary rat astrocytes: role of myosin light-chain kinase

1995 ◽  
Vol 269 (1) ◽  
pp. C257-C266 ◽  
Author(s):  
L. D. Shrode ◽  
J. D. Klein ◽  
W. C. O'Neill ◽  
R. W. Putnam
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Light Chain ◽  
Myosin Light Chain Kinase ◽  
Myosin Light Chain ◽  
Tetraacetic Acid ◽  
Maximal Inhibition ◽  
Kinase C ◽  
Rat Astrocytes

Primary rat astrocytes exposed to hyperosmotic solutions undergo Na(+)-dependent amiloride-sensitive alkalinization of 0.36 U [measured with the pH-sensitive fluorescent dye 2',7'-bis(carboxyethyl)-5(6)-carboxy-fluorescein], suggesting that shrinkage-induced alkalinization is due to activation of Na+/H+ exchange (NHE). Alkalinization is maintained for at least 20 min, and is readily reversible and ATP dependent. Hyperosmotic solutions produced no increase of intracellular Ca2+ or adenosine 3',5'-cyclic monophosphate (cAMP). Loading cells with 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, a Ca2+ chelator, or depleting cells of protein kinase C (PKC) had no effect on activation of NHE. Thus shrinkage-induced activation of NHE does not involve cAMP, Ca2+, or PKC. However, ML-7, an inhibitor of myosin light-chain kinase (MLCK), inhibited shrinkage-induced activation with a half-maximal inhibition of 56 microM. This activation was also inhibited by 500 microM N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, 100 microM chlorpromazine, and 50 microM trifluoperazine, all calmodulin inhibitors. Shrinkage increased the phosphorylation of an 18-kDa protein that colocalizes with myosin light chain. Our data suggest that shrinkage-induced activation of NHE in astrocytes occurs via a novel pathway involving activation of calmodulin-dependent MLCK and phosphorylation of myosin light chain.

scholarly journals Role of Rho kinase in myosin light chain phosphorylation induced by prostaglandin F2α in rabbit aorta

2000 ◽  
Vol 82 ◽  
pp. 206
Author(s):  
Rrika Shimomura ◽  
Takahiro Iwanaga ◽  
Minoru Seto ◽  
Yasuharu Sasaki ◽  
Katsuaki Ito
Keyword(s):  
Light Chain ◽  
Myosin Light Chain ◽  
Rho Kinase ◽  
Prostaglandin F2α ◽  
Rabbit Aorta ◽  
Myosin Light Chain Phosphorylation
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