scholarly journals Lipopolysaccharide differentially affects the osteogenic differentiation of periodontal ligament stem cells and bone marrow mesenchymal stem cells through Toll-like receptor 4 mediated nuclear factor κB pathway

2014 ◽  
Vol 5 (3) ◽  
pp. 67 ◽  
Author(s):  
Chenghua Li ◽  
Bei Li ◽  
Zhiwei Dong ◽  
Li Gao ◽  
Xiaoning He ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Periodontal Ligament ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Periodontal Ligament Stem Cells ◽  
Marrow Mesenchymal Stem Cells

Evaluation of toll-like receptor 4 expression in human bone marrow mesenchymal stem cells by lipopolysaccharides from Shigella

Biologicals ◽  
2018 ◽  
Vol 55 ◽  
pp. 53-58
Author(s):  
Mohammad Reza Hashemzadeh ◽  
Mohamadreza Baghaban Eslaminejad ◽  
Reza Salman Yazdi ◽  
Reza Aflatoonian
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Marrow Mesenchymal Stem Cells

scholarly journals Jawbone microenvironment promotes periodontium regeneration by regulating the function of periodontal ligament stem cells

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Bin Zhu ◽  
Wenjia Liu ◽  
Yihan Liu ◽  
Xicong Zhao ◽  
Hao Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Tissue Regeneration ◽  
Periodontal Ligament ◽  
Tooth Development ◽  
Material Surface ◽  
Cell Aggregates ◽  
Periodontal Ligament Stem Cells ◽  
Marrow Mesenchymal Stem Cells ◽  
Mineralized Matrix

AbstractDuring tooth development, the jawbone interacts with dental germ and provides the development microenvironment. Jawbone-derived mesenchymal stem cells (JBMSCs) maintain this microenvironment for root and periodontium development. However, the effect of the jawbone microenvironment on periodontium tissue regeneration is largely elusive. Our previous study showed that cell aggregates (CAs) of bone marrow mesenchymal stem cells promoted periodontium regeneration on the treated dentin scaffold. Here, we found that JBMSCs enhanced not only the osteogenic differentiation of periodontal ligament stem cells (PDLSCs) but also their adhesion to titanium (Ti) material surface. Importantly, the compound CAs of PDLSCs and JBMSCs regenerated periodontal ligament-like fibers and mineralized matrix on the Ti scaffold surface, both in nude mice ectopic and minipig orthotopic transplantations. Our data revealed that an effective regenerative microenvironment, reconstructed by JBMSCs, promoted periodontium regeneration by regulating PDLSCs function on the Ti material.

scholarly journals Comparison of the Expression of Periodontal Markers in Dental and Bone Marrow-derived Mesenchymal Stem Cells.

2020 ◽  
Vol 14 (1) ◽  
pp. 196-202
Author(s):  
Devy Garna ◽  
Manmeet Kaur ◽  
Francis J Hughes ◽  
Mandeep Ghuman
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Dental Pulp ◽  
Periodontal Ligament ◽  
Bone Marrow Cells ◽  
Dental Pulp Stem Cells ◽  
Periodontal Ligament Stem Cells ◽  
Osteogenic Induction ◽  
Pulp Cells

Background: Periodontal ligament stem cells are a source of mesenchymal stem cells, but it is unclear whether their phenotype is distinct from mesenchymal stem cells derived from different tissues, such as those derived from bone marrow. Objective: To investigate the expression of the putative PDL markers asporin, periostin, nestin and cementum protein 1, by periodontal ligament stem cells both constitutively and during osteogenic differentiation when compared to bone marrow-derived mesenchymal stem cells, and dental pulp stem cells. Methods: The primary human periodontal ligament, bone marrow, and dental pulp stem cells, and osteoblasts from different donors were cultured in vitro. The expression of periodontal marker associated genes during osteogenic induction was tested by qRT-PCR and immunofluorescence staining. Results: Asporin expression was detected in periodontal ligament stem cells and increased markedly during the time in culture (upregulated x53 fold at 21 days post-induction). During osteogenic differentiation, asporin expression significantly decreased in periodontal ligament cells whereas periostin significantly decreased in dental pulp cells. Periostin expression was absent in osteoblasts, but expression gradually increased in all other cells with time in culture. Nestin expression was mainly seen in the periodontal ligament and dental pulp cells and was largely absent in osteoblasts and bone marrow cells. Cementum protein-1 was most highly expressed in bone marrow cells and osteoblasts following osteogenic induction. Conclusions: The results provide further evidence that periodontal ligament-derived and bone marrow derived mesenchymal stem cells are phenotypically distinct. Periodontal markers are also expressed in dental pulp stem cells.

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