scholarly journals Association between ATP production and oxidative mtDNA damage through mitochondrial respiratory chain in the rat caecal ligation and puncture heart injury model

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 2) ◽  
pp. P387
Author(s):  
J Hirata ◽  
M Oya ◽  
J Kotani ◽  
T Yamada ◽  
A Hashimoto ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Mitochondrial Respiratory Chain ◽  
Atp Production ◽  
Mtdna Damage ◽  
Injury Model ◽  
Heart Injury ◽  
Caecal Ligation And Puncture ◽  
Caecal Ligation ◽  
Mitochondrial Respiratory

scholarly journals The road to the structure of the mitochondrial respiratory chain supercomplex

2020 ◽  
Vol 48 (2) ◽  
pp. 621-629
Author(s):  
Nikeisha J. Caruana ◽  
David A. Stroud
Keyword(s):  
Respiratory Chain ◽  
Large Body ◽  
Mitochondrial Respiratory Chain ◽  
Proton Pumping ◽  
Atp Production ◽  
Structural Characterisation ◽  
The Road ◽  
X Ray Crystallography ◽  
History Of ◽  
Mitochondrial Respiratory

The four complexes of the mitochondrial respiratory chain are critical for ATP production in most eukaryotic cells. Structural characterisation of these complexes has been critical for understanding the mechanisms underpinning their function. The three proton-pumping complexes, Complexes I, III and IV associate to form stable supercomplexes or respirasomes, the most abundant form containing 80 subunits in mammals. Multiple functions have been proposed for the supercomplexes, including enhancing the diffusion of electron carriers, providing stability for the complexes and protection against reactive oxygen species. Although high-resolution structures for Complexes III and IV were determined by X-ray crystallography in the 1990s, the size of Complex I and the supercomplexes necessitated advances in sample preparation and the development of cryo-electron microscopy techniques. We now enjoy structures for these beautiful complexes isolated from multiple organisms and in multiple states and together they provide important insights into respiratory chain function and the role of the supercomplex. While we as non-structural biologists use these structures for interpreting our own functional data, we need to remind ourselves that they stand on the shoulders of a large body of previous structural studies, many of which are still appropriate for use in understanding our results. In this mini-review, we discuss the history of respiratory chain structural biology studies leading to the structures of the mammalian supercomplexes and beyond.

Coenzyme Q: potentially useful index of bioenergetic and oxidative status of spermatozoa

1995 ◽  
Vol 41 (2) ◽  
pp. 217-219 ◽  
Author(s):  
A G Angelitti ◽  
L Colacicco ◽  
C Callà ◽  
M Arizzi ◽  
S Lippa
Keyword(s):  
Respiratory Chain ◽  
Coenzyme Q10 ◽  
Coenzyme Q ◽  
Mitochondrial Respiratory Chain ◽  
Oxidative Status ◽  
Atp Production ◽  
The Difference ◽  
Autoregulatory Mechanism ◽  
Infertile Patients ◽  
Mitochondrial Respiratory

Abstract The concentration of coenzyme Q10 (CoQ10), a key intermediate of the mitochondrial respiratory chain, was determined in spermatozoa of 13 fertile subjects, 8 potentially fertile patients, and 12 infertile patients. CoQ10 concentrations were significantly higher (P < 0.001) in infertile patients than in fertile and potentially fertile subjects. The difference between potentially fertile and fertile subjects was also significant (P < 0.001). We propose that a decrease in consumption of CoQ10 in both infertile and potentially fertile populations is due to an autoregulatory mechanism of ATP production.

scholarly journals Exogenous H2S reduces the acetylation levels of mitochondrial respiratory enzymes via regulating the NAD+-SIRT3 pathway in cardiac tissues of db/db mice

2019 ◽  
Vol 317 (2) ◽  
pp. E284-E297 ◽  
Author(s):  
Yu Sun ◽  
Zongyan Teng ◽  
Xiaojiao Sun ◽  
Linxue Zhang ◽  
Jian Chen ◽  
...  
Keyword(s):  
Respiratory Chain ◽  
Mitochondrial Respiratory Chain ◽  
Neonatal Rat ◽  
Mass Spectrometry Analysis ◽  
Rat Cardiomyocytes ◽  
Sodium Hydrosulfide ◽  
Acetylation Level ◽  
Atp Production ◽  
Cellular Models ◽  
Mitochondrial Respiratory

Hydrogen sulfide (H2S), a gaseous molecule, is involved in modulating multiple physiological functions, such as antioxidant, antihypertension, and the production of polysulfide cysteine. H2S may inhibit reactive oxygen species generation and ATP production through modulating respiratory chain enzyme activities; however, the mechanism of this effect remains unclear. In this study, db/db mice, neonatal rat cardiomyocytes, and H9c2 cells treated with high glucose, oleate, and palmitate were used as animal and cellular models of type 2 diabetes. The mitochondrial respiratory rate, respiratory chain complex activities, and ATP production were decreased in db/db mice compared with those in db/db mice treated with exogenous H2S. Liquid chromatography with tandem mass spectrometry analysis showed that the acetylation level of proteins involved in the mitochondrial respiratory chain were increased in the db/db mice hearts compared with those with sodium hydrosulfide (NaHS) treatment. Exogenous H2S restored the ratio of NAD+/NADH, enhanced the expression and activity of sirtuin 3 (SIRT3) and decreased mitochondrial acetylation level in cardiomyocytes under hyperglycemia and hyperlipidemia. As a result of SIRT3 activation, acetylation of the respiratory complexe enzymes NADH dehydrogenase 1 (ND1), ubiquinol cytochrome c reductase core protein 1, and ATP synthase mitochondrial F1 complex assembly factor 1 was reduced, which enhanced the activities of the mitochondrial respiratory chain activity and ATP production. We conclude that exogenous H2S plays a critical role in improving cardiac mitochondrial function in diabetes by upregulating SIRT3.

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