scholarly journals Neutrophil CD64 expression, a marker of sepsis/infection, can be performed on a hematology blood counter and has variable correlation to C-reactive protein, procalcitonin and soluble CD163

Critical Care ◽  
2007 ◽  
Vol 11 (Suppl 4) ◽  
pp. P20 ◽  
Author(s):  
Bruce Davis ◽  
Karen Becker ◽  
Henry Rinder ◽  
Kathleen Davis
Keyword(s):  
C Reactive Protein ◽  
Cd64 Expression ◽  
Reactive Protein ◽  
Soluble Cd163 ◽  
Neutrophil Cd64

Neutrophil CD64 Is an Improved Indicator of Infection or Sepsis in Emergency Department Patients

2006 ◽  
Vol 130 (5) ◽  
pp. 654-661 ◽  
Author(s):  
Bruce H. Davis ◽  
Stephen H. Olsen ◽  
Ejaz Ahmad ◽  
Nancy C. Bigelow
Keyword(s):  
Emergency Department ◽  
Sedimentation Rate ◽  
Diagnostic Tests ◽  
Diagnostic Performance ◽  
Tertiary Care ◽  
Clinical Score ◽  
C Reactive Protein ◽  
Cd64 Expression ◽  
Reactive Protein ◽  
Neutrophil Cd64

Abstract Context.—Sepsis, affecting millions of individuals annually with an associated high mortality rate, is among the top 10 causes of death. In addition, improvements in diagnostic tests for detecting and monitoring sepsis and infection have been limited in the last 25 years. Neutrophil CD64 expression has been proposed as an improved diagnostic test for the evaluation of infection and sepsis. Objective.—To evaluate the diagnostic performance of a quantitative flow cytometric assay for leukocyte CD64 expression in comparison with the standard tests for infection/sepsis in an ambulatory care setting. Design.—Prospective analysis of 100 blood samples from patients from an emergency department setting in a 965-bed tertiary care suburban community hospital was performed for neutrophil CD64 expression, C-reactive protein, erythrocyte sedimentation rate, and complete blood count. The laboratory findings were compared with a clinical score for the likelihood of infection/sepsis, which was obtained by a blinded retrospective chart review. Results.—The diagnostic performance, as gauged by the clinical score, varied with neutrophil CD64 (sensitivity 87.9%, specificity 71.2%, efficiency 76.8%) and outperformed C-reactive protein (sensitivity 88.2%, specificity 59.4%, efficiency 69.4%), absolute neutrophil count (sensitivity 60.0%, specificity 50.8%, efficiency 53.8%), myeloid left shift (sensitivity 68.2%, specificity 76.3%, efficiency 73.3%), and sedimentation rate (sensitivity 50.0%, specificity 65.5%, efficiency 61.0%). Conclusion.—Neutrophil CD64 expression quantitation provides improved diagnostic detection of infection/sepsis compared with the standard diagnostic tests used in current medical practice.

Neutrophil and monocyte receptor expression in patients with sepsis: implications for diagnosis and prognosis of sepsis

2019 ◽  
Vol 77 (6) ◽  
Author(s):  
Mariam Onsy F Hanna ◽  
Asmaa M Abdelhameed ◽  
Amany A Abou-Elalla ◽  
Reem M Hassan ◽  
Inas Kostandi
Keyword(s):  
Mortality Risk ◽  
Clinical Syndrome ◽  
Receptor Expression ◽  
C Reactive Protein ◽  
Surface Receptors ◽  
Reactive Protein ◽  
Diagnosis And Prognosis ◽  
Neutrophil Cd64 ◽  
Combination Approach ◽  
Insight Into

ABSTRACT Understanding the complex immune responses in sepsis is crucial to provide insight into the clinical syndrome. We evaluated the changes in the surface receptors of the cells of innate immunity, neutrophils and monocytes, in patients with sepsis. Since sepsis remains a clinical challenge, we aimed to assess the significance of altered receptor expression in diagnosis and prognosis. Critically ill patients with sepsis (n=31) were investigated for the expression of receptors for IgG heavy chain CD64 and CD16 on neutrophils and CD64 and the lipopolysaccharide receptor CD14 on monocytes by flow cytometry and compared to 23 patients with no sepsis. Patients with sepsis had increased expression of neutrophil CD64. Neutrophil CD64 was specific for discriminating patients with sepsis but showed weak sensitivity. When integrated in a scoring system, neutrophil CD64 in combination with C-reactive protein (CRP) and SOFA score showed a diagnostic accuracy of 0.93 for sepsis and significantly predicted increased mortality risk. While neutrophil CD16 did not discriminate for sepsis, decreased expression was associated with increased mortality risk. In contrast, monocyte CD64 and CD14 expression was unaltered in sepsis and was not associated with mortality risk. Our study demonstrates that unlike monocytes, neutrophil receptor expression is altered in patients with sepsis receiving intensive care. It is promising to apply a combination approach to diagnose sepsis especially in time-limited conditions.

scholarly journals Correction to: Soluble CD163, adiponectin, C-reactive protein and progression of dysglycaemia in individuals at high risk of type 2 diabetes mellitus: the ADDITION-PRO cohort

Diabetologia ◽  
2017 ◽  
Vol 61 (2) ◽  
pp. 498-503
Author(s):  
Pia Deichgræber ◽  
Daniel R. Witte ◽  
Holger J. Møller ◽  
Mette V. Skriver ◽  
Bjørn Richelsen ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
High Risk ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Soluble Cd163

scholarly journals Prognostic Value And Daily Trend Of Interleukin-6, Neutrophil CD64 Expression, C-Reactive Protein And Lipopolysaccharide-Binding Protein In Critically Ill Patients: Reliable Predictors Of Outcome Or Not?

2015 ◽  
Vol 34 (4) ◽  
pp. 431-439 ◽  
Author(s):  
Dragan Djordjevic ◽  
Janko Pejovic ◽  
Maja Surbatovic ◽  
Jasna Jevdjic ◽  
Sonja Radakovic ◽  
...  
Keyword(s):  
Severe Sepsis ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Interleukin 6 ◽  
Prognostic Value ◽  
C Reactive Protein ◽  
Lipopolysaccharide Binding Protein ◽  
Cd64 Expression ◽  
Reactive Protein ◽  
Lipopolysaccharide Binding

SummaryBackground:Severe sepsis and/or trauma complicated by multiple organ dysfunction syndrome are the leading causes of death in critically ill patients. The aim of this prospective single-centre study was to assess the prognostic value and daily trend of interleukin-6 (IL-6), neutrophil CD64 expression, C-reactive protein (CRP) and lipopolysaccharide-binding protein (LBP) regarding outcome in critically ill patients with severe trauma and/or severe sepsis. Outcome measure was hospital mortality.Methods:One hundred and two critically ill patients admitted to the intensive care unit of a tertiary university hospital were enrolled in this prospective study. Blood samples were collected on admission (day 1), days 2 and 3.Results:CD64 index was 1.6-fold higher on day 1 and 1.78-fold higher on day 2 in non-survivors (p<0.05). The area under the curve (AUC) for the CD64 index on day 1 for outcome was 0.727. At a cut-off level of 2.80 sensitivity was 75% and specificity was 65%. Patients with CD64 index level on day 1 higher than 2.80 had 2.4-fold higher probability of dying. Odds ratio is 2.40; 95% CI 0.60–9.67.Conclusions:CD64 index on day 1 is a fairly good predictor of outcome. AUCs for IL-6, CRP and LBP were < 0.55, suggesting these biomarkers failed to predict outcome.

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