scholarly journals Molecular mechanism of glutamine induction of HSP70 involves activation of the O-linked-N-acetylglucosamine pathway in murine embryonic fibroblast cells

Critical Care ◽  
2007 ◽  
Vol 11 (Suppl 2) ◽  
pp. P8
Author(s):  
C Hamiel ◽  
S Pinto ◽  
K Singleton ◽  
P Wischmeyer
Keyword(s):  
Molecular Mechanism ◽  
Fibroblast Cells ◽  
Murine Embryonic Fibroblast ◽  
Embryonic Fibroblast

Calcium hypochlorite on mouse embryonic fibroblast cells (NIH3T3) in vitro cytotoxicity and genotoxicity: MTT and comet assay

2020 ◽  
Vol 47 (7) ◽  
pp. 5377-5383
Author(s):  
Şehnaz Yilmaz ◽  
Oguz Yoldas ◽  
Aysin Dumani ◽  
Gizem Guler ◽  
Seda Ilgaz ◽  
...  
Keyword(s):  
Comet Assay ◽  
Mouse Embryonic Fibroblast ◽  
In Vitro Cytotoxicity ◽  
Fibroblast Cells ◽  
Calcium Hypochlorite ◽  
Embryonic Fibroblast

Prokaryotic Expression and Anti-IBDV Activity of Chicken Interleukin-18 and Interferon-γ

2017 ◽  
Vol 153 (1) ◽  
pp. 36-45 ◽  
Author(s):  
Baifen Song ◽  
Xiaoting Li ◽  
Jinzhu Ma ◽  
Liquan Yu ◽  
Zhenyue Feng ◽  
...  
Keyword(s):  
Natural Killer Cells ◽  
Natural Killer ◽  
Prokaryotic Expression ◽  
Interferon Γ ◽  
Fibroblast Cells ◽  
Killer Cells ◽  
Chicken Embryonic Fibroblast ◽  
Prokaryotic Expression Vector ◽  
Ifn Γ ◽  
Embryonic Fibroblast

Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified. To determine their antiviral activities, 200 ng/mL of ChIL-18 and/or ChIFN-γ were inoculated into chicken embryonic fibroblast cells. After 24 h, one 50% tissue culture infective dose (TCID50) of infectious bursal disease virus (IBDV) was inoculated into the chicken embryonic fibroblast cells. The results showed that the antiviral effect of ChIL-18 and ChIFN-γ in combination was better than that of ChIL-18 or ChIFN-γ alone. Next, 14-day-old chicken were injected with 200 µg of ChIL-18 and/or ChIFN-γ and then were challenged with 103 TCID50 of IBDV via intraperitoneal injection. The results showed that the proliferation of IBDV was inhibited by the injection of the recombinant proteins, especially the combination of ChIL-18 and ChIFN-γ, as evidenced by cytokine detection, quantitative PCR, and pathology analyses. These results indicate that ChIL-18 and ChIFN-γ could inhibit IBDV infection and the combination of ChIL-18 and ChIFN-γ has a better inhibitory effect than either cytokine alone.

scholarly journals Slow growth and unstable ribosomal RNA lacking pseudouridine in mouse embryonic fibroblast cells expressing catalytically inactive dyskerin

FEBS Letters ◽  
2013 ◽  
Vol 587 (14) ◽  
pp. 2112-2117 ◽  
Author(s):  
Bai-Wei Gu ◽  
Jingping Ge ◽  
Jian-Meng Fan ◽  
Monica Bessler ◽  
Philip J. Mason
Keyword(s):  
Ribosomal Rna ◽  
Slow Growth ◽  
Mouse Embryonic Fibroblast ◽  
Fibroblast Cells ◽  
Embryonic Fibroblast
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