scholarly journals Mesenchymal stem cell and endothelial cell interaction restores endothelial permeability via paracrine hepatocyte growth factor in vitro

Critical Care ◽  
2015 ◽  
Vol 19 (Suppl 1) ◽  
pp. P236
Author(s):  
Q Chen ◽  
A Liu ◽  
H Qiu ◽  
Y Yang
Keyword(s):  
Stem Cell ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Mesenchymal Stem Cell ◽  
Hepatocyte Growth Factor ◽  
Endothelial Permeability ◽  
Cell Interaction ◽  
Endothelial Cell Interaction ◽  
Hepatocyte Growth

scholarly journals Hepatocyte growth factor mediates mesenchymal stem cell–induced recovery in multiple sclerosis models

2012 ◽  
Vol 15 (6) ◽  
pp. 862-870 ◽  
Author(s):  
Lianhua Bai ◽  
Donald P Lennon ◽  
Arnold I Caplan ◽  
Anne DeChant ◽  
Jordan Hecker ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Stem Cell ◽  
Growth Factor ◽  
Mesenchymal Stem Cell ◽  
Hepatocyte Growth Factor ◽  
Hepatocyte Growth

scholarly journals Hepatocyte growth factor is a potent angiogenic factor which stimulates endothelial cell motility and growth.

1992 ◽  
Vol 119 (3) ◽  
pp. 629-641 ◽  
Author(s):  
F Bussolino ◽  
M F Di Renzo ◽  
M Ziche ◽  
E Bocchietto ◽  
M Olivero ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Endothelial Cell ◽  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Cell Monolayer ◽  
Angiogenic Factor ◽  
Tyrosine Kinase Receptor ◽  
Collagen Gels ◽  
Hepatocyte Growth

Hepatocyte Growth Factor (HGF, also known as Scatter Factor) is a powerful mitogen or motility factor in different cells, acting through the tyrosine kinase receptor encoded by the MET protooncogene. Endothelial cells express the MET gene and expose at the cell surface the mature protein (p190MET) made of a 50 kD (alpha) subunit disulfide linked to a 145-kD (beta) subunit. HGF binding to endothelial cells identifies two sites with different affinities. The higher affinity binding site (Kd = 0.35 nM) corresponds to the p190MET receptor. Sub-nanomolar concentrations of HGF, but not of a recombinant inactive precursor, stimulate the receptor kinase activity, cell proliferation and motility. HGF induces repairs of a wound in endothelial cell monolayer. HGF stimulates the scatter of endothelial cells grown on three-dimensional collagen gels, inducing an elongated phenotype. In the rabbit cornea, highly purified HGF promotes neovascularization at sub-nanomolar concentrations. HGF lacks activities related to hemostasis-thrombosis, inflammation and endothelial cells accessory functions. These data show that HGF is an in vivo potent angiogenic factor and in vitro induces endothelial cells to proliferate and migrate.

scholarly journals ID: 1074 The efficiency of adipose-derived stem cell pretreated with hepatocyte growth factor and platelet rich plasma on a liver cirrhosis mouse model

2017 ◽  
Vol 4 (S) ◽  
pp. 157
Author(s):  
Yen Thi-Kim Nguyen ◽  
Nam Hai Nguyen ◽  
Trinh Van Le ◽  
Ngoc Hong Vo ◽  
Thanh Minh Dang ◽  
...  
Keyword(s):  
Liver Cirrhosis ◽  
Stem Cell ◽  
Growth Factor ◽  
Mouse Model ◽  
Hepatocyte Growth Factor ◽  
Platelet Rich Plasma ◽  
The Body ◽  
Adipose Derived Stem Cell ◽  
Hepatocyte Growth

Background: Because of their ease of isolation, high proliferation capacity in vitro, as well as their ability to differentiate into liver cells, Adipose-derived stem cell (ADSCs), are considered to be a promised candidate for liver disease treatment, including liver cirrhosis treatment. Recent studies show that hepatocyte growth factor (HGF) can stimulate ADSC to migrate to the injured areas and platelet rich plasma (PRP) can increase the stemness of ADSCs. Method: In this study, we cultured ADSCs in the medium supplemented 20 ng/ml and 10% PRP; then transplanted them into the cirrhosis mouse model. After 11 weeks of CCl4 induction (1ml/kg, three times per week), mice were divided into 3 groups: (1) PBS group which were injected 0.2 ml PBS; (2) ADSC group which were transplanted 5x105 ADSCs non-pretreated with HGF and PRP; (3) ADSC/HGF+PRP group which were transplanted 5x105 ADSCs pretreated with HGF and PRP via the tail vein. We evaluated the effectiveness of the therapeutic at the day 7th and 14th after transplantation. Results: The results show that the transplantation of ADSC pretreated with HGF and PRP after seven days improves the body weight (increase 4.673%); decreases the ALT level (p < 0.05), the total leukocyte number (p < 0.05) and the expression of Pro-collagen (decrease 4.1 times) as well as α-SMA (decrease 5.1 times), in comparison to the ADSC group. ADSCs pretreated with HGF and PRP also help to improve the liver tissue structure. Conclusion: The therapy using ADSCs pretreated with HGF and PRP is considered to be a promising therapy.

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