scholarly journals An Agar Diffusion Method for the Determination Of Antibodies Against Staphylococcus Aureus Deoxyribonuclease

1977 ◽  
Vol 18 (4) ◽  
pp. 480-493
Author(s):  
Roar Gudding
Keyword(s):  
Staphylococcus Aureus ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Agar Diffusion Method

Antibacterial Activity of Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa

2017 ◽  
Vol 8 (03) ◽  
Author(s):  
Kyoung- Sun Seo ◽  
Seong Woo Jin ◽  
Seongkyu Choi ◽  
Kyeong Won Yun
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
The Other ◽  
Diffusion Method ◽  
E Coli ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

UJI AKTIVITAS ANTIBAKTERI FORMULASI GEL UNTUK SARIAWAN DARI EKSTRAK DAUN SAGA ( Abrus precatorius Linn. ) TERHADAP BAKTERI Staphylococcus aureus

2017 ◽  
Vol 2 (2) ◽  
pp. 239
Author(s):  
Ratih Dyah Pertiwi ◽  
Joni Kristanto ◽  
Graha Ayu Praptiwi
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Chemical Constituents ◽  
Optimal Concentration ◽  
Diffusion Method ◽  
Abrus Precatorius ◽  
Agar Diffusion ◽  
Medicinal Properties ◽  
Agar Diffusion Method ◽  
Gel Preparation

One of plants used by Indonesian people as a traditional medicine is saga plant (Abrus precatorius L.). This plant has medicinal properties as medication for thrush, cough and laryngitis. Chemical constituents contained in sage leaves which work as antibacterial are flavonoid and saponin. This study aims to determine the optimal concentration of saga leaves extract which can be formulated in a gel dosage form with qualified physical evaluation and has antibacterial activity against Staphylococcus aureus. Saga leaves extract is prepared by maceration method using ethanol 70% as solvent, and then the extract obtained is preliminarily tested to see its antibacterial activity against Staphylococcus aureus with agar diffusion method. Gel formulation for thrush is made with dispersion method in three formulas with variants of active substance concentration that is FI (1%), FII (3%) and FIII (5%). Gel preparation antibacterial activity test is conducted with agar diffusion method as a plate cylinder. Based on this research, it was found that the extract of saga leaves which is positively made is efficacious as antibacterial and can be formulated into a gel preparation for thrush with optimal concentration in F III (5%), this is indicated by widest diameter of the inhibition area against Staphylococus aureus bacteria

scholarly journals Assessment in Vitro of Antibacterial Activity of Manipulated Product, on Solution Form, Obtained from Dry Extract of Leaves of Psidium guajava L.

2015 ◽  
Vol 8 (1) ◽  
pp. 64
Author(s):  
Marcos V.S. Silva ◽  
Tayara S. Frazão ◽  
Melyssa M.A.V. Ferreira ◽  
Jandir S. Sales ◽  
Denis B. Vieira ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Cytotoxic Activity ◽  
Staphylococcus Epidermidis ◽  
Psidium Guajava ◽  
Diffusion Method ◽  
Dry Extract ◽  
Agar Diffusion ◽  
Agar Diffusion Method

<p class="Default">The <em>Psidium guajava L</em>. specie is a perennial shrub, belonging to the <em>Myrtaceae</em> family and it is popularly known as guava, its leaves are used in therapy for treating various diseases. The study aims to evaluate the antibacterial activity <em>in vitro </em>of manipulated product obtained from dried extract of the leaves of <em>P. guajava </em>L. front standard bacteria ATCC and clinical isolates. The tests were conducted on bacterial samples: <em>Staphylococcus aureus </em>(ATCC 25923), <em>Pseudomonas aeruginosa </em>(ATCC 27883), <em>Escherichia coli </em>(ATCC 25922), <em>Salmonella spp</em>, <em>Acinetobacter baumannii</em>, <em>Proteus mirabilis</em>, <em>Shigella flexneri</em>, <em>Staphylococcus epidermidis</em>, <em>Staphylococcus haemolyticus</em>, <em>Streptococcus agalactiae, Streptococcus mutans</em>. Among the tests can be mentioned phytochemical of the ethanol extract (EE), microbiological control and physical-chemical analysis of the product and microbiological tests such as agar diffusion method (wells), minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), an evaluation test of hemolytic capacity of the solution and in vitro assay cytotoxic activity were performed, . The best result of the product in the agar diffusion method was front <em>Staphylococcus epidermidis</em>, while the lower MIC and MBC were front <em>Staphylococcus aureus </em>(ATCC 25923). The product showed no hemolytic activity and no cytotoxic activity at the tested concentrations. According to the test results, it is believed on the possibility of the production of a pharmaceutical formulation derived from the dry extract of <em>Psidium guajava</em>, since it showed great antibacterial activity.</p>

scholarly journals Thermonuclease seroinhibition test for distinguishing Staphylococcus aureus from other coagulase-positive staphylococci

1979 ◽  
Vol 9 (1) ◽  
pp. 141-143
Author(s):  
R Victor ◽  
F Lachica ◽  
S S Jang ◽  
P D Hoeprich
Keyword(s):  
Staphylococcus Aureus ◽  
Diffusion Method ◽  
Human Origin ◽  
Agar Diffusion ◽  
Agar Diffusion Method ◽  
Coagulase Positive Staphylococci ◽  
Serological Specificity

Since coagulase-positive staphylococci from animals are heterogeneous, another test is necessary to distinguish Staphylococcus aureus from them. Staphylococcal thermonucleases appear to be heterogeneous; antisera raised against S. aureus isolated from humans inhibit thermonuclease activity as demonstrated by the metachromatic well-agar diffusion method. The serological specificity of the thermonuclease elaborated by S. aureus of human origin was demonstrated using three antisera and 407 strains of staphylococci from diverse human and animal sources.

scholarly journals The Effect of Ethanol Extract of Yam Bulbs (Pachyrhizus Erosus L Urb) on the Growth of Staphylococcus Aureus Bacteria Agar Diffusion Method

2021 ◽  
Vol 2 (1) ◽  
pp. 20-26
Author(s):  
Slamet
Keyword(s):  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Clean Water ◽  
Inhibitory Zone ◽  
Agar Diffusion ◽  
Pachyrhizus Erosus ◽  
Quasi Experimental ◽  
Agar Diffusion Method ◽  
Tuber Extract

Bengkuang Plant (Pachyrhizus erosus L. Urb) is a plant that can be used as herbal medicine. The community-made uses yam into powder by drying the place that is not exposed to direct sun, then pounded into a powder. This powdered yam is used by mixing clean water and used as powder. Bulbs are proven to contain flavonoid compounds, isoflavonoid, saponin, and daidzein, which can function as antibatter. The purpose of this study was to explain the concentration of tuber extract of ethanol tuber (Pachyrhizus erosus L. Urb) on the growth of Staphylococcus aureus bacteria with the agar diffusion method. The research design used in this research is quasi-experimental (Quasi-Experimental). The sample of this research is extract of bamboo tuber ethanol (Pachyrhizus erosus L. Urb) with concentration of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80% and 90%. Based on the result of the research, the measurement of the inhibitory zone of Staphylococcus aureus bacteria was found to average 6,00 mm at a concentration of 10% -40%, 7.33 mm at concentration 50%, 7.67 mm at concentration 60%, 9.33 at concentration 70 %, 10.67 mm at concentrations of 80% and 13.33 mm at a concentration of 90%. The data were analyzed by Kendall's Tau test (p = 0,000 <0,05), so it can be concluded that there is an influence of extract of bamboo tuber ethanol (Pachyrhizus erosus L. Urb) on the growth of Staphylococcus aureus bacteria agar diffusion method.

scholarly journals Evaluation of the antimicrobial effect of calcium hydroxide combined to different vehicles

2016 ◽  
Vol 31 (2) ◽  
pp. 89
Author(s):  
Tatiana Dantas Piana ◽  
Maria de Fátima Malvar Gesteira ◽  
Erica Dos Santos Carvalho ◽  
Josilene Borges Torres Lima Matos ◽  
Monica Franca ◽  
...  
Keyword(s):  
Calcium Hydroxide ◽  
Enterococcus Faecalis ◽  
Antimicrobial Effect ◽  
Antimicrobial Action ◽  
Diffusion Method ◽  
Agar Diffusion ◽  
Inhibition Zones ◽  
Agar Diffusion Method

Objective: To evaluate the potentiating effect of different substances in antimicrobial action of calcium hydroxide.Methods: The agar diffusion method, was used with well technique, to analyze seven substances associated to calcium hydroxide to make some pastes, they are: chlorexidine, saline, anesthetic, malvatricin, propolis, hypochlorite, paramonochlorophenol.Results: The pastes with malvatricin and paramonochlorophenol presented greater inhibition zones against Enterococcus faecalis.Conclusion: There was potentialization in antimicrobial effect of calcium hydroxide when associated to paramonochlorophenol and to malvatricin especially against Enterococcus faecalis. Low increase of the antimicrobial capacity was observed when using chlorexidine as vehicle to calcium hydroxine paste.

scholarly journals Potential study of Dillenia serrata Thunb. fruit extract from Bali Botanical Garden’s collection

2021 ◽  
Vol 26 (2) ◽  
pp. 79-84
Author(s):  
I Putu Agus Hendra Wibawa ◽  
I Nyoman Lugrayasa ◽  
Sutomo Sutomo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Local Community ◽  
Raw Material ◽  
Diffusion Method ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Dried Fruit ◽  
Agar Diffusion Method

Dillenia serrata Thunb.is a member of the Dillenia clan which is endemic to Sulawesi. D. serrata fruit is a seasonal fruit and can be used as a cooking ingredient as a sour taste, raw material for several food products such as candied fruit and syrup, and has the potential to be processed into chips and jam. Traditionally, the leaves and bark of D. serrata are also used by the local community as a sprue medicine, fever, wound medicine, treating swelling or inflammation and treating vomiting of blood. It is believed that there are still many untapped benefits from this plant. This study was conducted to determine the effectiveness of D. serrata fruit extract as an antimicrobial that causes disease in humans, and to determine its effectiveness as an antioxidant. The antioxidant test was carried out using the DPPH method while the antimicrobial test was carried out by the agar diffusion method (Kirby-Bauer). The ripe Dillenia fruit was extracted using methanol, the extract was then diluted in various concentrations for the DPPH test The results showed that the D. serrata extract had the ability as an antioxidant. The antioxidant activity of fresh fruit extracts was higher than that of dried fruit extracts. D. serrata extract is effective in inhibiting the growth of Pseudomonas aeruginosa, Staphylococcus aureus, and Streptococcus mutans bacteria.

scholarly journals Agar Diffusion Method for the Assay of Colicins

1968 ◽  
Vol 16 (10) ◽  
pp. 1468-1474 ◽  
Author(s):  
H. Richardson ◽  
A. H. Emslie-Smith ◽  
B. W. Senior
Keyword(s):  
Diffusion Method ◽  
Agar Diffusion ◽  
Agar Diffusion Method
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