scholarly journals Monoclonal Antibodies Applied in an Immunoperoxidase Method for Detection of Parvovirus in Specimens of Small Intestine from Dog and Mink

1988 ◽  
Vol 29 (2) ◽  
pp. 263-264
Author(s):  
Lars Jönsson ◽  
Cajsa Magnusson ◽  
MajLis Book ◽  
Nils Juntti
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Immunoperoxidase Method

Development and evaluation of an immunoenzymometric assay for the chicken progesterone receptor

1991 ◽  
Vol 129 (2) ◽  
pp. 189-196 ◽  
Author(s):  
M. K. Bläuer ◽  
P. J. Tuohimaa ◽  
P. J. Vilja
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Horseradish Peroxidase ◽  
Progesterone Receptor ◽  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Coefficients Of Variation ◽  
First Time ◽  
Determination Range

ABSTRACT A specific and sensitive immunoenzymometric assay (IEMA) was developed for measuring the quantity of chicken progesterone receptor (PR) in tissue cytosol. The assay uses two monoclonal antibodies to the PR. One is used to capture the PR. The second (labelled with biotin) reacts first with the captured receptor and subsequently with avidin-labelled horseradish peroxidase to provide an enzymatic end-point. The method has a determination range from 0·3 to 60 pmol/l. Intra- and interassay coefficients of variation were 3·7% and 9·0% respectively. The assay can be performed with equal results as a rapid (3 h) or an overnight procedure. The IEMA is convenient, especially for signal measurement and the calculation of results. No ultracentrifugation of samples is needed, since the IEMA can be performed on low-speed cytosol samples. Assay results correlated well (r = 0·927) with those obtained by the conventional ligand-binding assay used in our laboratory. Similar results were obtained with the IEMA and the ligand-binding assay after exposure of cytosol samples to increased temperatures: at 20 °C the PR remained stable for the 4-h period examined, whereas at 37 °C almost complete degradation of the PR was observed in 30 min. Being more than 100 times as sensitive as the ligand-binding assay, the IEMA enabled the quantification of PR for the first time in such tissues as the bursa and small intestine even of immature animals. Journal of Endocrinology (1991) 129, 189–196

scholarly journals Function of integrin in duodenal mucosal uptake of iron

Blood ◽  
1993 ◽  
Vol 81 (2) ◽  
pp. 517-521 ◽  
Author(s):  
ME Conrad ◽  
JN Umbreit ◽  
RD Peterson ◽  
EG Moore ◽  
KP Harper
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Binding Protein ◽  
Iron Binding ◽  
Immunofluorescent Microscopy ◽  
Mucosal Surfaces ◽  
Iron Binding Protein ◽  
Mucosal Uptake

Abstract A mechanism for the absorption of inorganic iron in the small intestine is described in which integrins appear to play an important role in the passage of iron across microvillous membranes. Biochemical isolates from microvillous preparations of duodenum from rats dosed with radioiron showed radioactivity concentrated in integrins. The presence of integrins on mucosal surfaces of duodenal cells was confirmed by immunofluorescent microscopy using anti-integrin monoclonal antibodies. Immunoprecipitation methods were used to show that microvillous radioiron was precipitated with anti-integrin antibodies and that mobilferrin, a 56-Kd cytosol iron-binding protein, coprecipitated with integrins. We postulate from these data that the mucosal uptake of iron from the gut lumen is mediated via an integrin-mobilferrin pathway.

scholarly journals Analysis of B-cell antigens in normal reactive lymphoid tissue using four B-cell monoclonal antibodies

Blood ◽  
1983 ◽  
Vol 62 (4) ◽  
pp. 775-783
Author(s):  
FM Hofman ◽  
E Yanagihara ◽  
B Byrne ◽  
R Billing ◽  
S Baird ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
B Cells ◽  
Lymph Nodes ◽  
B Cell ◽  
Germinal Center ◽  
Immunoperoxidase Method ◽  
Intense Staining ◽  
Normal Spleen ◽  
Reactive Hyperplasia

Lymph nodes from 13 cases of reactive hyperplasia were examined with four different monoclonal antibodies to B cells. B-1 recognizes an antigen of 30,000 daltons on B cells. CB-2 was prepared with normal spleen and binds to a glycolipid. BA-1 labels surface immunoglobulin- positive cells, but not T lymphocytes or monocytes. B-532 recognizes an antigenic determinant of 45,000 daltons. Using the immunoperoxidase method on frozen sections of reactive lymph nodes, the staining patterns of these four unique antibodies showed dramatic differences. B- 1 labeled 80%-90% of the germinal center cells and 10%-50% of the mantle region. Few interfollicular cells were positive. CB-2 stained predominantly in the mantle area (50%-90% positive cells), with moderate staining in the germinal center as well and less than 1% positive cells in the diffuse cortex. BA-1 exhibited predominant labeling in the mantle (50%-90%), with little staining in the germinal center. A large number of cells in the interfollicular, subcapsular, and medullary regions expressed the BA-1 antigen. The B-5 antibody demonstrated intense staining in the follicle (50%-95%). This staining often appeared to be polarized within the germinal center. The mantle zone demonstrated staining of 30%-50% of the cells. The different staining patterns of the B-cell antibodies, as demonstrated by the in situ distribution of positive cells within the lymph node, may reflect stages of development or activation of the B-cell population.

scholarly journals Heterogeneity of rat macrophages recognized by monoclonal antibodies: an immunohistochemical and immunoelectron microscopic study.

1989 ◽  
Vol 37 (5) ◽  
pp. 635-641 ◽  
Author(s):  
M Takeya ◽  
L Hsiao ◽  
Y Shimokawa ◽  
K Takahashi
Keyword(s):  
Monoclonal Antibodies ◽  
Langerhans Cells ◽  
Peritoneal Macrophages ◽  
Immunoperoxidase Method ◽  
Reaction Products ◽  
Tissue Sections ◽  
Cytoplasmic Vacuoles ◽  
A Cell ◽  
Thymic Medulla ◽  
Splenic Red Pulp

Three monoclonal antibodies, designated RM-1, TRPM-1, and TRPM-2, were raised against rat peritoneal macrophages. By the immunoperoxidase method, antigens recognized by these antibodies were distributed throughout most tissue and free macrophages examined, including those of splenic red pulp, lymphatic sinus, connective tissue, and peritoneal cavity, as well as Kupffer cells of liver and alveolar macrophages. The numbers of positive cells were different for each antibody. RM-1 and TRPM-1 were also reactive with interdigitating cells (IDCs) in the thymus-dependent area and with Langerhans cells in the skin, whereas TRPM-2 failed to demonstrate IDCs in thymic medulla and Langerhans cells. The reactions of each antibody were observed by immunoelectron microscopy in the different ultrastructural compartments of the cells. RM-1 recognized a cell surface antigen; reaction products for TRPM-1 were found on a part of the cell membrane and in the cytoplasmic vacuoles; and those of TRPM-2 were present along the nuclear envelope and intracytoplasmic vacuoles. These antibodies seem to be useful not only for the detection of macrophages in tissue sections but also for investigation of macrophage heterogeneity in different tissues.

scholarly journals LOCALIZATION OF LYSOZYME IN NORMAL RAT TISSUES BY AN IMMUNOPEROXIDASE METHOD

1974 ◽  
Vol 22 (3) ◽  
pp. 139-146 ◽  
Author(s):  
MATTI KLOCKARS ◽  
ELLIOTT F. OSSERMAN
Keyword(s):  
Small Intestine ◽  
Lymph Node ◽  
Alveolar Macrophages ◽  
Paneth Cells ◽  
Proximal Tubules ◽  
Rat Tissues ◽  
Immunoperoxidase Method ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Normal Rat

The immoperoxidase-immunoglobulin bridge technique has been utilized for the localization of lysozyme (LZM) in the cells and tissue of the normal rat. Specific LZM staining was found in the proximal tubules of the kidney, Paneth cells of the small intestine and alveolar macrophages. LZM staining was also demonstrated in macrophages in the perifollicular sinusoids of an activated lymph node. The immunoperoxidase method appears to have significant advantages over previously described methods for LZM localization, and it should also be adaptable to electron microscopic studies.

scholarly journals Specific norovirus interaction with Lewis x and Lewis a on human intestinal inflammatory mucosa during refractory inflammatory bowel disease

2020 ◽  
Author(s):  
Georges Tarris ◽  
Alexis de Rougemont ◽  
Marie Estienney ◽  
Maeva Charkaoui ◽  
Thomas Mouillot ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Inflammatory Bowel Disease ◽  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Bowel Disease ◽  
Lewis X ◽  
Sialyl Lewis ◽  
Colon And Rectum ◽  
Inflammatory Bowel ◽  
Lewis A

ABSTRACTInflammatory bowel disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis (UC), is related to immunological and microbial factors with the possible implication of enteric viruses. We characterize the interaction between human noroviruses (HuNoVs) and blood group antigens in refractory CD and UC using HuNoV Virus Like Particles (VLPs) and histological tissues. Immunohistochemistry was conducted on inflammatory tissue samples from the small intestine, colon and rectum in 15 CD and 9 UC patients. Analysis of the regenerative mucosa of the colon and rectum revealed strong expression of sialylated Lewis a (sLea) and Lewis x (sLex) antigens, and HuNoV VLP binding in the absence of ABO antigen expression in both UC and CD. Competition experiments using sialidase, lectins and monoclonal antibodies demonstrated that HuNoV attachment mostly involved Lea and to a lesser extent, Lex moieties on regenerative mucosa in both UC and CD. Further studies will be required to understand the implications of specific HuNoV binding to regenerative mucosa in refractory IBD.IMPORTANCEInflammatory Bowel Disease (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are progressive diseases affecting millions of people each year. Flare-ups during IBD result in severe mucosal alterations of the small intestine (CD), colon and rectum (CD and UC). Immunohistochemical analysis of CD and UC samples showed strong expression of known tumoral markers, Sialyl-Lewis a (CA19.9) and Sialyl-Lewis x (CD15s) antigens on colonic and rectal regenerative mucosa, concurrent with strong human norovirus (HuNov) VLP GII.4 affinity. Sialidase treatment and competition experiments using HBGA-specific monoclonal antibodies and lectins clearly demonstrated the implication of the Lewis a moiety, and to a lesser extent Lewis x, in HuNov recognition in regenerative mucosa of CD and UC tissues. Further studies are required to explore the possible implications of enteric viruses in the impairment of epithelial repair and dysregulation of inflammatory pathways during severe IBD.

scholarly journals EVALUATION OF SMALL INTESTINE FUNCTION IN INFANTS WITH PERSISTENT DIARRHEA (PD) USING MONOCLONAL ANTIBODIES (MAbs)

1994 ◽  
Vol 36 (6) ◽  
pp. 827-827
Author(s):  
M Araya ◽  
J Espinoza ◽  
F Alliende ◽  
O Brunser ◽  
J Rebollo ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Persistent Diarrhea
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