scholarly journals Enterotoxin Production at 4, 22, And 37°C by Yersinia Enterocolitica and Yersinia Enterocolitica-Like Bacteria Isolated from Porcine Tonsils and Pork Products

1985 ◽  
Vol 26 (1) ◽  
pp. 13-20
Author(s):  
Truls Nesbakken
Keyword(s):  
Yersinia Enterocolitica ◽  
Pork Products ◽  
Enterotoxin Production

scholarly journals Identification and Characterization of Pathogenic Yersinia enterocolitica Isolates by PCR and Pulsed-Field Gel Electrophoresis

2005 ◽  
Vol 71 (7) ◽  
pp. 3674-3681 ◽  
Author(s):  
S. Thisted Lambertz ◽  
M.-L. Danielsson-Tham
Keyword(s):  
Multiplex Pcr ◽  
Yersinia Enterocolitica ◽  
Gel Electrophoresis ◽  
Pulsed Field Gel Electrophoresis ◽  
Virulence Plasmid ◽  
Pork Meat ◽  
Pulsed Field ◽  
Pork Products ◽  
Food Borne ◽  
Pork Samples

ABSTRACT Approximately 550 to 600 yersiniosis patients are reported annually in Sweden. Although pigs are thought to be the main reservoir of food-borne pathogenic Yersinia enterocolitica, the role of pork meat as a vehicle for transmission to humans is still unclear. Pork meat collected from refrigerators and local shops frequented by yersiniosis patients (n = 48) were examined for the presence of pathogenic Yersinia spp. A combined culture and PCR method was used for detection, and a multiplex PCR was developed and evaluated as a tool for efficient identification of pathogenic food and patient isolates. The results obtained with the multiplex PCR were compared to phenotypic test results and confirmed by pulsed-field gel electrophoresis (PFGE). In all, 118 pork products (91 raw and 27 ready-to-eat) were collected. Pathogenic Yersinia spp. were detected by PCR in 10% (9 of 91) of the raw pork samples (loin of pork, fillet of pork, pork chop, ham, and minced meat) but in none of the ready-to-eat products. Isolates of Y. enterocolitica bioserotype 4/O:3 were recovered from six of the PCR-positive raw pork samples; all harbored the virulence plasmid. All isolates were recovered from food collected in shops and, thus, none were from the patients' home. When subjected to PFGE, the six isolates displayed four different NotI profiles. The same four NotI profiles were also present among isolates recovered from the yersiniosis patients. The application of a multiplex PCR was shown to be an efficient tool for identification of pathogenic Y. enterocolitica isolates in naturally contaminated raw pork.

Enterotoxin Production in Two-Culture Media by Yersinia enterocolitica Isolated from Sausages, Porcine Ceca and Tongues in San Luis, Argentina

1991 ◽  
Vol 54 (1) ◽  
pp. 47-49
Author(s):  
ANA MARIA S. DE GUZMAN ◽  
NORMA B. B. DE PEDERIVA ◽  
LUIS S. MAYORGA ◽  
BLAS MICALIZZI
Keyword(s):  
Body Weight ◽  
Yersinia Enterocolitica ◽  
Culture Media ◽  
Infant Mouse ◽  
San Luis ◽  
Heat Stable ◽  
Enterotoxin Production ◽  
Casamino Acids

Twenty-six strains of Yersinia enterocolitica isolated from fresh sausages, bovine tongues, and porcine ceca and tongues were evaluated for the production of heat-stable enterotoxin (ST, infant mouse test) in trypticase soy broth (TSB) and casamino acids broth (CAB). The gut weight/body weight ratios obtained with the two media were similar. Sixteen strains produced ST in both media; nine strains did not. Strain 23 produced ST in CAB but not in TSB.

Comparison of Two Enrichment Protocols for the Detection of Yersinia in Slaughtered Pigs and Pork Products

1990 ◽  
Vol 53 (12) ◽  
pp. 1047-1049 ◽  
Author(s):  
JACOB KWAGA ◽  
JOHN O. IVERSEN ◽  
JAMES R. SAUNDERS
Keyword(s):  
Yersinia Enterocolitica ◽  
Potassium Chlorate ◽  
Enrichment Method ◽  
Enrichment Medium ◽  
Pork Products ◽  
Enrichment Methods ◽  
Slaughtered Pigs

Two enrichment methods were evaluated in the course of a study to determine the occurrence of Yersinia enterocolitica in slaughtered pigs and pork products. Eighty Yersinia strains belonging to one of four species were recovered. Of the 67 strains of Y. enterocolitica encountered, 48 belonged to known pathogenic bioserotypes. The enrichment medium incorporating irgasan, ticarcillin, and potassium chlorate (ITC) was found to be superior to the two-step enrichment method (YER/BOS) for isolation of pathogenic Y. enterocolitica from throat swabs. When pork products were examined using the two methods, YER/BOS was by far superior for recovery of yersiniae, although all strains isolated by this method belonged to nonpathogenic bioserotypes and Y. enterocolitica-like organisms. Conversely, ITC enrichment recovered fewer strains but most were pathogenic bioserotypes. Thus, swine can serve as a reservoir of virulent Y. enterocolitica in Saskatchewan, and ITC enrichment is recommended for the isolation of these strains.

scholarly journals Pork safety – challenges and opportunities

2021 ◽  
Vol 854 (1) ◽  
pp. 012043
Author(s):  
Nedjeljko Karabasil ◽  
Tamara Boskovic ◽  
Dragan Vasilev ◽  
Nikola Betic ◽  
Mirjana Dimitrijevic
Keyword(s):  
Food Safety ◽  
Toxoplasma Gondii ◽  
Yersinia Enterocolitica ◽  
Meat Inspection ◽  
Production Chain ◽  
Pork Production ◽  
Pork Products ◽  
Meat Safety ◽  
Challenges And Opportunities ◽  
Safe Food

Abstract As pork and pork products represent an important part of the diet, the issue of pork safety and quality has become more prominent. Food safety concerns are shaping consumers’ attitudes toward safe food. The farm and meat sectors aim at producing healthy animals in a protected environment, which is a key point for food/meat safety. The most common biological hazards in the pork production chain are Salmonella spp., Yersinia enterocolitica, Trichinella spp. and Toxoplasma gondii. These hazards are not detectable by conventional meat inspection, and measures rely on prevention or reduction of contamination along the production chain.

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