scholarly journals Do early premalignant changes in normal breast epithelial cells predict cancer development?

2004 ◽  
Vol 7 (1) ◽  
Author(s):  
Robert B Clarke ◽  
Nigel J Bundred
Keyword(s):  
Epithelial Cells ◽  
Normal Breast ◽  
Cancer Development ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

scholarly journals Expression of estrogen receptor β isoforms in normal breast epithelial cells and breast cancer: regulation by methylation

Oncogene ◽  
2003 ◽  
Vol 22 (48) ◽  
pp. 7600-7606 ◽  
Author(s):  
Chunyan Zhao ◽  
Eric W-F Lam ◽  
Andrew Sunters ◽  
Eva Enmark ◽  
Manuela Tamburo De Bella ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Epithelial Cells ◽  
Normal Breast ◽  
Estrogen Receptor Β ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

Characterization of normal breast epithelial cells in primary cultures: Differentiation and growth factor receptors studies

1992 ◽  
Vol 28 (11-12) ◽  
pp. 716-724 ◽  
Author(s):  
Philippe Berthon ◽  
Gianfranco Pancino ◽  
Patricia Cremoux ◽  
Alberto Roseto ◽  
Christian Gespach ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Primary Cultures ◽  
Growth Factor Receptors ◽  
Normal Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

Human chorionic gonadotropin and a 15 amino acid hCG fragment of the hormone induce downregulation of the cytokine IL-8 receptor in normal breast epithelial cells

2011 ◽  
Vol 6 (3) ◽  
Author(s):  
Samuel M.R. Noronha ◽  
Silvana A.A. Correa-Noronha ◽  
Irma H. Russo ◽  
Ricardo López de Cicco ◽  
Julia Santucci-Pereira ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Normal Breast ◽  
Breast Cancer Cell Lines ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

AbstractCytokine receptors are associated with tumor cell growth by increasing proliferation, metastasis and regulating self-renewal of cancer stem cells (SCs). There is a strong association between cytokine IL-8 receptor (CXCR1) over-expression and cells displaying SC characteristics. Human chorionic gonadotropin (hCG) causes differentiation, inhibition of cell proliferation and increased apoptosis of the breast epithelium. hCG receptor (LHCGR) expression in breast tumors and in breast cancer cell lines is undetectable or low. In this study, our objective was to assess and compare the effects of hCG and a 15 amino acid hCG fragment of the hormone on mRNA expression of CXCR1 and LHCGR on normal breast epithelial cells (MCF-10F) by real time RT-PCR after treatment with hCG or a hCG fragment for 15 days. Cell proliferation was also measured. hCG and the hCG fragment decreased cell proliferation in both groups. The compounds upregulated LHCGR expression and downregulated CXCR1 expression. It is possible to postulate that an increase of LHCGR mRNA seems to respond to the decrease of CXCR1 expression. These genes probably act synergistically to reduce the amount of cancer SCs in the mammary gland. Thereby, the use of hCG or the hCG fragment as a therapeutic or preventive tool should be considered.

scholarly journals Spatial correlation of cell stiffness and traction forces in cancer cells measured with combined SICM and TFM

RSC Advances ◽  
2021 ◽  
Vol 11 (23) ◽  
pp. 13951-13956
Author(s):  
Johannes Rheinlaender ◽  
Hannes Wirbel ◽  
Tilman E. Schäffer
Keyword(s):  
Epithelial Cells ◽  
Traction Force ◽  
Living Cells ◽  
Normal Breast ◽  
Cell Stiffness ◽  
Force Density ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Local Stiffness ◽  
Normal Breast Epithelial Cells

Using combined SICM and TFM we identified a subcellular correlation between the local stiffness and traction force density in living cells. We found this correlation in normal breast epithelial cells, but not in cancerous breast epithelial cells.

Cytochemical and biochemical evidence of cathepsin B in malignant, transformed and normal breast epithelial cells

1987 ◽  
Vol 87 (1) ◽  
pp. 145-154
Author(s):  
E. Krepela ◽  
J. Bartek ◽  
D. Skalkova ◽  
J. Vicar ◽  
D. Rasnick ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cathepsin B ◽  
Proteinase Inhibitors ◽  
Cysteine Proteinase ◽  
Normal Breast ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells

Human breast cancer cell lines, as well as transformed mammary epithelial cells (HBL-100) and growth-stimulated normal breast epithelial cells showed positive cytochemical reaction with the proteinase substrate 2-(N-benzyloxycarbonyl-L-arginyl-L-arginylamido)-4-methoxynapht halene, in the presence of 5-nitrosalicylaldehyde. The reaction product, small fluorescent granules, was distributed throughout the cytoplasm, in the perinuclear zone, in some cytoplasmic projections, and at the cell surface. Using a panel of various proteinase inhibitors, we found that the formation of the reaction product was an enzymic function of a cysteine proteinase. Using the substrate 7-(N-benzyloxycarbonyl-L-arginyl-L-arginylamido)-4-methylcoumarin, we evaluated some biochemical properties of the cysteine proteinase, including pH-activity profile, pH stability, apparent relative molecular mass and sensitivity toward various proteinase inhibitors. We found that the proteinase from the studied breast epithelial cells exhibited characteristics of a mature form of cathepsin B. Taken together, the cytochemical and biochemical data provide evidence that human breast epithelial cells of cancer origin, as well as in the transformed or growth-stimulated state express active cathepsin B and compartmentalize it into specific subcellular sites.

Normal Breast Epithelial Cells Induce Apoptosis of MCF-7 Breast Cancer Cells through a p53-Mediated Pathway

2000 ◽  
Vol 3 (6) ◽  
pp. 338-344 ◽  
Author(s):  
Robert-Alain Toillon ◽  
Eric Adriaenssens ◽  
Danièle Wouters ◽  
Severine Lottin ◽  
Bénoni Boilly ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Epithelial Cells ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Normal Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells ◽  
Mcf 7

Antigenic heterogeneity of breast cell lines detected by monoclonal antibodies and its relationship with the cell cycle

1985 ◽  
Vol 73 (1) ◽  
pp. 321-333
Author(s):  
P.A. Edwards ◽  
R.A. Skilton ◽  
A.W. Payne ◽  
M.G. Ormerod
Keyword(s):  
Cell Cycle ◽  
Monoclonal Antibodies ◽  
Epithelial Cells ◽  
Cell Lines ◽  
Antigen Expression ◽  
Normal Breast ◽  
Breast Epithelial Cells ◽  
Breast Epithelial ◽  
Normal Breast Epithelial Cells ◽  
Antigenic Heterogeneity

Established cell lines were stained by immunofluorescence with four monoclonal antibodies to study the phenomenon of antigenic heterogeneity and its possible relation to the cell cycle. Five cell lines thought to be of breast origin, MCF-7, ZR-75-1, T47D, MDA-MB-231 and HBL-100, were stained with three monoclonal antibodies (LICR-LON-M8, LICR-LON-M18, LICR-LON-M24) that each stain a distinct subset of normal breast epithelial cells, i.e. demonstrate antigenic heterogeneity in normal breast epithelial cells. Contrasting monoclonal antibodies LICR-LON-FIB75 and LICR-LON-FIB86 to homogeneously expressed antigens were also used. For the antibodies demonstrating heterogeneity distinct positive and negative fractions were not seen by flow cytometry; the intensity of fluorescence varied continuously from background to a hundred times stronger than background. Fluorescent DNA staining showed no obvious relation between antigen expression and the cell cycle. The essentially constant proportion of cells of a given antigenic phenotype in the various phases suggests that these antibodies do not distinguish a phenotype associated with a distinct proliferating population of cells.

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