scholarly journals Development of a transgenic mouse line for the evaluation of the androgen receptor activity in vivo

2003 ◽  
Vol 5 (S1) ◽  
Author(s):  
R Attar ◽  
C Cullinan ◽  
C-P Ho ◽  
M Swerdel ◽  
J Dell ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Transgenic Mouse ◽  
Receptor Activity ◽  
Mouse Line ◽  
Transgenic Mouse Line ◽  
Androgen Receptor Activity

scholarly journals Nas transgenic mouse line allows visualization of Notch pathway activity in vivo

genesis ◽  
2006 ◽  
Vol 44 (6) ◽  
pp. 277-286 ◽  
Author(s):  
Céline Souilhol ◽  
Sarah Cormier ◽  
Marie Monet ◽  
Sandrine Vandormael-Pournin ◽  
Anne Joutel ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Notch Pathway ◽  
Mouse Line ◽  
Pathway Activity ◽  
Transgenic Mouse Line

scholarly journals Transmission of tauopathy strains is independent of their isoform composition

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Zhuohao He ◽  
Jennifer D. McBride ◽  
Hong Xu ◽  
Lakshmi Changolkar ◽  
Soo-jung Kim ◽  
...  
Keyword(s):  
Human Brain ◽  
Transgenic Mouse ◽  
Mouse Line ◽  
Cell Type ◽  
Transgenic Mouse Line ◽  
Adult Human ◽  
Tau Isoforms ◽  
Underlying Mechanisms ◽  
Mouse Lines

AbstractThe deposition of pathological tau is a common feature in several neurodegenerative tauopathies. Although equal ratios of tau isoforms with 3 (3R) and 4 (4R) microtubule-binding repeats are expressed in the adult human brain, the pathological tau from different tauopathies have distinct isoform compositions and cell type specificities. The underlying mechanisms of tauopathies are unknown, partially due to the lack of proper models. Here, we generate a new transgenic mouse line expressing equal ratios of 3R and 4R human tau isoforms (6hTau mice). Intracerebral injections of distinct human tauopathy brain-derived tau strains into 6hTau mice recapitulate the deposition of pathological tau with distinct tau isoform compositions and cell type specificities as in human tauopathies. Moreover, through in vivo propagation of these tau strains among different mouse lines, we demonstrate that the transmission of distinct tau strains is independent of strain isoform compositions, but instead intrinsic to unique pathological conformations.

scholarly journals Interleukin-6 stimulation of growth of prostate cancer in vitro and in vivo through activation of the androgen receptor

2009 ◽  
Vol 16 (1) ◽  
pp. 155-169 ◽  
Author(s):  
Kamilla Malinowska ◽  
Hannes Neuwirt ◽  
Ilaria T Cavarretta ◽  
Jasmin Bektic ◽  
Hannes Steiner ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Interleukin 6 ◽  
Tumour Progression ◽  
Receptor Activity ◽  
Mitogen Activated Protein Kinase ◽  
Androgen Receptor Activity ◽  
Stimulation Of

It is hypothesized that ligand-independent activation of the androgen receptor is one of the mechanisms implicated in tumour progression. However, supportive evidence is limited to the effect of HER-2/neu that stimulates prostate cancer progression through activation of the androgen receptor. In the present study, we have asked whether the proinflammatory cytokine interleukin-6 (IL-6), which is known to stimulate androgen receptor activity and expression of its downstream target genes, may also induce growth of androgen-sensitive cells. We have found that IL-6 differentially regulates proliferation of LAPC-4 and MDA PCa 2b cells. In MDA PCa 2b cells, growth stimulation by IL-6 was reversed by administration of either the non-steroidal anti-androgen bicalutamide or the inhibitor of the mitogen-activated protein kinase pathway PD98059. Neither cell line was found to express endogenous IL-6. Interestingly, the treatment of those prostate cancer cells did not increase phosphorylation of STAT3. The effect of IL-6 on stimulation of androgen receptor activity in MDA PCa 2b cells was lower than that of androgen, comparable with findings reported by other researchers. However, growth of MDA PCa 2b xenografts in castrated animals treated with IL-6 was similar to that in non-castrated animals. In addition, bicalutamide showed an inhibitory effect on IL-6-regulated growth in vivo. Taken together, data in the present study demonstrate that IL-6 may cause growth of androgen receptor-positive tumours in vitro and in vivo through activation of the androgen receptor.

scholarly journals A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo

PLoS ONE ◽  
2015 ◽  
Vol 10 (6) ◽  
pp. e0128198 ◽  
Author(s):  
Krung Phiwpan ◽  
Jie Guo ◽  
Wei Zhang ◽  
Tanyu Hu ◽  
Bhargavi M. Boruah ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Mouse Line ◽  
Transgenic Mouse Line

scholarly journals A Tendon-Specific Double Reporter Transgenic Mouse Enables Tracking Cell Lineage and Functions Alteration In Vitro and In Vivo

2021 ◽  
Vol 22 (20) ◽  
pp. 11189
Author(s):  
Rui Chen ◽  
Xunlei Zhou ◽  
Thomas Skutella
Keyword(s):  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Cell Lineage ◽  
Mouse Line ◽  
Reporter System ◽  
Specific Expression ◽  
Transgenic Mouse Line ◽  
Blue Fluorescent Protein

We generated and characterized a transgenic mouse line with the tendon-specific expression of a double fluorescent reporter system, which will fulfill an unmet need for animal models to support real-time monitoring cell behaviors during tendon development, growth, and repair in vitro and in vivo. The mScarlet red fluorescent protein is driven by the Scleraxis (Scx) promoter to report the cell lineage alteration. The blue fluorescent protein reporter is expressed under the control of the 3.6kb Collagen Type I Alpha 1 Chain (Col1a1) proximal promoter. In this promoter, the existence of two promoter regions named tendon-specific cis-acting elements (TSE1, TSE2) ensure the specific expression of blue fluorescent protein (BFP) in tendon tissue. Collagen I is a crucial marker for tendon regeneration that is a major component of healthy tendons. Thus, the alteration of function during tendon repair can be estimated by BFP expression. After mechanical stimulation, the expression of mScarlet and BFP increased in adipose-derived mesenchymal stem cells (ADMSCs) from our transgenic mouse line, and there was a rising trend on tendon key markers. These results suggest that our tendon-specific double reporter system is a novel model used to study cell re-differentiation and extracellular matrix alteration in vitro and in vivo.

scholarly journals The long non-coding RNA PCGEM1 is regulated by androgen receptor activity in vivo

2015 ◽  
Vol 14 (1) ◽  
pp. 46 ◽  
Author(s):  
Abhijit Parolia ◽  
Francesco Crea ◽  
Hui Xue ◽  
Yuwei Wang ◽  
Fan Mo ◽  
...  
Keyword(s):  
Androgen Receptor ◽  
Receptor Activity ◽  
Non Coding Rna ◽  
Androgen Receptor Activity ◽  
Long Non Coding Rna

Abstract 133: Novel Transgenic Mouse Lines for Identification of Cardiomyocyte Nuclei and Visualization of Their Cell Cycle Status In-vitro and In-vivo

2014 ◽  
Vol 115 (suppl_1) ◽  
Author(s):  
Alexandra Raulf ◽  
Hannes Horder ◽  
Caroline Geisen ◽  
Sabine Grünberg ◽  
Patricia Freitag ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Transgenic Mouse ◽  
Terminal Differentiation ◽  
Mouse Line ◽  
Biological Mechanisms ◽  
Regeneration Potential ◽  
Technical Limitation ◽  
Adult Heart ◽  
Transgenic Mouse Line

The typical remodelling process after cardiac injury is scarring and compensatory hypertrophy. The limited regeneration potential of the adult heart is due to the post-mitotic status of cardiomyocytes (CMs), which are mostly binucleated. Nevertheless, there is evidence for CM turnover in the adult heart, but its extent is still under debate. One technical limitation of quantitations is the unequivocal identification of CMs and of CM cell division. In order to enable a clear identification of CM nuclei in-vivo, we have developed a transgenic mouse line in which a fusion protein of the human histone 2B and the red fluorescence protein mCherry is specifically and persistently expressed in CM nuclei (αMHC-H2B-mCh). The fluorescence label allowed the investigation of CM percentages in native tissue slices, which properly reflect the cellular composition of the heart. We focused on regional (atrium versus left and right ventricle, apical versus basal slices) and developmental-stage dependent changes in the percentage of CM nuclei and binuclearity. We therefore analyzed time-points before/during and after terminal differentiation of CMs (postnatal day 3 (P3), P7 and 9 weeks). In addition, we also investigated endoreduplication and acytokinetic mitosis using time lapse microscopy in postnatal CMs to better understand cell biological mechanisms leading to terminal differentiation. As currently huge efforts are invested for the search of substances that increase the regeneration potential of the heart, we established a novel screening assay for cell-cycle modifying substances in isolated, postnatal CMs. We crossed the αMHC-H2B-mCh with the CAG-eGFPanillin mouse line, which marks cell-cycle activity with a high resolution of M-phase. Analysis of binuclearity and of different eGFPanillin subcellular localizations will be helpful to understand, whether CMs complete cytokinesis. As a proof of principle we investigated the effects of cell cycle activating micro RNAs199 and 590. Thus, our double transgenic mouse line will be useful to examine the plasticity of mono- and binuclear CMs and to unravel cell biological mechanisms leading to terminal differentiation of CMs.

scholarly journals A New Transgenic Mouse Line for Imaging Mitochondrial Calcium Signals

Function ◽  
2021 ◽  
Vol 2 (3) ◽  
Author(s):  
Nelly Redolfi ◽  
Elisa Greotti ◽  
Giulia Zanetti ◽  
Tino Hochepied ◽  
Cristina Fasolato ◽  
...  
Keyword(s):  
Transgenic Mouse ◽  
Fluorescent Protein ◽  
Ex Vivo ◽  
Brain Slices ◽  
Resonance Energy ◽  
Mouse Line ◽  
Isolated Cells ◽  
Genomic Locus ◽  
Transgenic Mouse Line

AbstractMitochondria play a key role in cellular calcium (Ca2+) homeostasis. Dysfunction in the organelle Ca2+ handling appears to be involved in several pathological conditions, ranging from neurodegenerative diseases, cardiac failure and malignant transformation. In the past years, several targeted green fluorescent protein (GFP)-based genetically encoded Ca2+ indicators (GECIs) have been developed to study Ca2+ dynamics inside mitochondria of living cells. Surprisingly, while there is a number of transgenic mice expressing different types of cytosolic GECIs, few examples are available expressing mitochondria-localized GECIs, and none of them exhibits adequate spatial resolution. Here we report the generation and characterization of a transgenic mouse line (hereafter called mt-Cam) for the controlled expression of a mitochondria-targeted, Förster resonance energy transfer (FRET)-based Cameleon, 4mtD3cpv. To achieve this goal, we engineered the mouse ROSA26 genomic locus by inserting the optimized sequence of 4mtD3cpv, preceded by a loxP-STOP-loxP sequence. The probe can be readily expressed in a tissue-specific manner upon Cre recombinase-mediated excision, obtainable with a single cross. Upon ubiquitous Cre expression, the Cameleon is specifically localized in the mitochondrial matrix of cells in all the organs and tissues analyzed, from embryos to aged animals. Ca2+ imaging experiments performed in vitro and ex vivo in brain slices confirmed the functionality of the probe in isolated cells and live tissues. This new transgenic mouse line allows the study of mitochondrial Ca2+ dynamics in different tissues with no invasive intervention (such as viral infection or electroporation), potentially allowing simple calibration of the fluorescent signals in terms of mitochondrial Ca2+ concentration ([Ca2+]).

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