scholarly journals Regulation of mammary gland branching morphogenesis by the extracellular matrix and its remodeling enzymes

2003 ◽  
Vol 6 (1) ◽  
Author(s):  
Jimmie E Fata ◽  
Zena Werb ◽  
Mina J Bissell
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Branching Morphogenesis

scholarly journals A pleiotropic role for FGF signaling in mammary gland stromal fibroblasts

2019 ◽  
Author(s):  
Zuzana Koledova ◽  
Jakub Sumbal
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Mammary Epithelium ◽  
Branching Morphogenesis ◽  
Mammary Epithelial ◽  
Fibroblast Proliferation ◽  
Fgf Signaling ◽  
Stromal Fibroblasts ◽  
Fibroblast Migration ◽  
And Migration

AbstractFibroblast growth factor (FGF) signaling is crucial for mammary gland development. While multiple roles for FGF signaling in the epithelium were described, the function of FGF signaling in mammary stroma has not been elucidated. In this study, we investigated FGF signaling in mammary fibroblasts. We found that mammary fibroblasts express FGF receptors 1 and 2 and respond to FGF ligands. In particular, FGF2 and FGF9 induce sustained ERK1/2 signaling and promote fibroblast proliferation and migration in 2D. Intriguingly, only FGF2 induces fibroblast migration in 3D extracellular matrix (ECM) through regulation of actomyosin cytoskeleton and promotes force-mediated collagen remodeling by mammary fibroblasts. Moreover, FGF2 regulates production of ECM proteins by mammary fibroblasts, including collagens, fibronectin, osteopontin, and matrix metalloproteinases. Finally, we show that FGF2 signaling in mammary fibroblasts enhances fibroblast-induced branching of mammary epithelium. Our results demonstrate a pleiotropic role for FGF signaling in mammary fibroblasts with implications for regulation of mammary stromal functions and epithelial branching morphogenesis.Summary statementFGF signaling in mammary fibroblasts regulates fibroblast proliferation, migration, extracellular matrix production and remodeling, and fibroblast-mediated mammary epithelial branching morphogenesis.

scholarly journals A Potential Role for MMPs during the Formation of Non-Neurogenic Placodes

2018 ◽  
Vol 6 (3) ◽  
pp. 20 ◽  
Author(s):  
Paige Drake ◽  
Tamara Franz-Odendaal
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Matrix Metalloproteinases ◽  
Potential Role ◽  
Critical Role ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling ◽  
Lens Development ◽  
Cell Behaviors ◽  
Placode Formation

The formation of non-neurogenic placodes is critical prior to the development of several epithelial derivatives (e.g., feathers, teeth, etc.) and their development frequently involves morphogenetic proteins (or morphogens). Matrix metalloproteinases (MMPs) are important enzymes involved in extracellular matrix remodeling, and recent research has shown that the extracellular matrix (ECM) can modulate morphogen diffusion and cell behaviors. This review summarizes the known roles of MMPs during the development of non-neurogenic structures that involve a placodal stage. Specifically, we discuss feather, hair, tooth, mammary gland and lens development. This review highlights the potential critical role MMPs may play during placode formation in these systems.

Participation of two different mesenchymes in the developing mouse mammary gland: synthesis of basement membrane components by fat pad precursor cells

Development ◽  
1985 ◽  
Vol 89 (1) ◽  
pp. 243-257
Author(s):  
Koji Kimata ◽  
Teruyo Sakakura ◽  
Yutaka Inaguma ◽  
Masato Kato ◽  
Yasuaki Nishizuka
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Flank Region ◽  
Mouse Mammary Gland ◽  
Precursor Cells ◽  
Basement Membranes ◽  
Immunofluorescent Staining ◽  
Fat Pad ◽  
Matrix Products ◽  
Membrane Components

Two different types of mesenchyme, fat pad precursor cells (FP) and fibroblastic cells (MM) are involved in the morphogenesis of mammary gland epithelium of mouse embryo. Especially, an interaction between FP and the epithelium is necessary for its characteristic shaping of ductal branching structure. To assess the relative participations of the mesenchymes, we have analysed the extracellular matrix products by immunofluorescent staining method using antibodies to laminin, proteoheparan sulphate, and fibronectin. The staining patterns suggested that, after the 16th day of gestation when fatty substances first appeared in FP and the epithelial rudiments started to elongate and branch rapidly, FP initiated synthesis of laminin and proteoheparan sulphate, while MM synthesized fibronectin at all times. Attention was also paid to differences in the epithelial basement membranes (BM) concomitant with ones in the mesenchyme. BM were always stained with antibodies to laminin and proteoheparan sulphate. However, topographical differences in thickness were observed: the one facing FP, often seen at the tip region of the end bud, was thin, while the other surrounded by MM, often at the flank region of the duct, was thick. Specific elaboration of BM-like extracellular matrix products by FP may attribute to observed differences in BM thickness which are related to the characteristic shaping of the mammary gland.

Foxp3 heterozygosity does not overtly affect mammary gland development during puberty or the oestrous cycle in mice

2020 ◽  
Vol 32 (8) ◽  
pp. 774
Author(s):  
Vahid Atashgaran ◽  
Pallave Dasari ◽  
Leigh J. Hodson ◽  
Andreas Evdokiou ◽  
Simon C. Barry ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Mammary Gland ◽  
Mammary Gland Development ◽  
Cancer Susceptibility ◽  
Branching Morphogenesis ◽  
Genetic Mutation ◽  
Oestrous Cycle ◽  
Normal Mammary Gland ◽  
Foxp3 Mrna ◽  
Gland Development

Female mice heterozygous for a genetic mutation in transcription factor forkhead box p3 (Foxp3) spontaneously develop mammary cancers; however, the underlying mechanism is not well understood. We hypothesised that increased cancer susceptibility is associated with an underlying perturbation in mammary gland development. The role of Foxp3 in mammary ductal morphogenesis was investigated in heterozygous Foxp3Sf/+ and wildtype Foxp3+/+ mice during puberty and at specific stages of the oestrous cycle. No differences in mammary ductal branching morphogenesis, terminal end bud formation or ductal elongation were observed in pubertal Foxp3Sf/+ mice compared with Foxp3+/+ mice. During adulthood, all mice underwent normal regular oestrous cycles. No differences in epithelial branching morphology were detected in mammary glands from mice at the oestrus, metoestrus, dioestrus and pro-oestrus stages of the cycle. Furthermore, abundance of Foxp3 mRNA and protein in the mammary gland and lymph nodes was not altered in Foxp3Sf/+ mice compared with Foxp3+/+ mice. These studies suggest that Foxp3 heterozygosity does not overtly affect mammary gland development during puberty or the oestrous cycle. Further studies are required to dissect the underlying mechanisms of increased mammary cancer susceptibility in Foxp3Sf/+ heterozygous mice and the function of this transcription factor in normal mammary gland development.

scholarly journals Barx2 and Fgf10 regulate ocular glands branching morphogenesis by controlling extracellular matrix remodeling

Development ◽  
2011 ◽  
Vol 138 (15) ◽  
pp. 3307-3317 ◽  
Author(s):  
C. Tsau ◽  
M. Ito ◽  
A. Gromova ◽  
M. P. Hoffman ◽  
R. Meech ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Branching Morphogenesis ◽  
Extracellular Matrix Remodeling ◽  
Matrix Remodeling

scholarly journals Integrins as architects of cell behavior

2016 ◽  
Vol 27 (19) ◽  
pp. 2885-2888 ◽  
Author(s):  
Charles H. Streuli
Keyword(s):  
Extracellular Matrix ◽  
Mammary Gland ◽  
Cell Surface ◽  
Cell Function ◽  
External Environment ◽  
Cell Behavior ◽  
Cell Surface Receptors ◽  
Surface Receptors ◽  
Mammary Gland Differentiation

Integrins are cell surface receptors that bind cells to their physical external environment, linking the extracellular matrix to cell function. They are essential in the biology of all animals. In the late 1980s, we discovered that integrins are required for the ability of breast epithelia to do what they are programmed to do, which is to differentiate and make milk. Since then, integrins have been shown to control most other aspects of phenotype: to stay alive, to divide, and to move about. Integrins also provide part of the mechanism that allows cells to form tissues. Here I discuss how we discovered that integrins control mammary gland differentiation and explore the role of integrins as central architects of other aspects of cell behavior.

Abstract 5251: Branching morphogenesis in the mammary gland is regulated by sprouty-2

2012 ◽  
Author(s):  
Valgardur Sigurdsson ◽  
Saevar Ingthorsson ◽  
Bylgja Hilmarsdottir ◽  
Sigridur R. Franzdottir ◽  
Eirikur Steingrimsson ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Branching Morphogenesis
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