scholarly journals Recent advances in technologies for the detection of occult metastatic cells in bone marrow of breast cancer patients

2001 ◽  
Vol 3 (5) ◽  
Author(s):  
Stephan Braun ◽  
Nadia Harbeck
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Cancer Patients ◽  
Breast Cancer Patients ◽  
Recent Advances ◽  
Metastatic Cells

Expression of Heterochromatin Protein 1 in the Primary Tumor of Breast Cancer Patients in the Presence or Absence of Occult Metastatic Cells in the Bone Marrow

2008 ◽  
Vol 23 (4) ◽  
pp. 219-224 ◽  
Author(s):  
A.P.S. Abreu ◽  
C. Milani ◽  
M.L.H. Katayama ◽  
E.M. Barbosa ◽  
L. Gomes da Fonseca ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Gene Expression ◽  
Bone Marrow ◽  
Cancer Patients ◽  
Breast Cancer Patients ◽  
Rt Pcr ◽  
Heterochromatin Protein 1 ◽  
Heterochromatin Protein ◽  
Heterochromatin Formation ◽  
Metastatic Cells

Gene silencing may occur in breast cancer samples from patients presenting with occult metastatic cells in the bone marrow and one mechanism regulating gene suppression is heterochromatin formation. We have studied whether members of the heterochromatin protein 1 family (HP1Hsα, HP1Hsβ and HP1Hsγ), which take part in chromatin packaging and gene expression regulation, were differentially expressed in tumors from patients with and without occult metastatic cells in their bone marrow. Tumor samples and bone marrow aspirates were obtained from 37 breast cancer patients. Median age was 63 years and 68% of the patients presented with clinical stage I/II disease. Presence of occult metastatic cells in bone marrow was detected through keratin-19 expression by nested RT-PCR in samples from 20 patients (54.1%). The presence of occult metastatic cells in bone marrow was not associated with node involvement, histological grade, estrogen receptor and ERBB2 immunoexpression. Relative gene expression of HP1Hsα, HP1Hsβ and HP1Hsγ was determined by real-time RT-PCR and did not vary according to the presence of occult metastatic cells in bone marrow. In addition, the combined expression of these three transcripts could not be used to classify samples according to the presence of bone marrow micrometastasis. Our work indicates that regulation of heterochromatin formation through HP1 family members may not be the sole mechanism implicated in the metastatic process to the bone marrow.

Persistent occult metastatic cells in bone marrow of breast cancer patients may require antibody therapy

2001 ◽  
Vol 37 ◽  
pp. S51
Author(s):  
W. Janni ◽  
B. Strobl ◽  
D. Rjosk ◽  
Ch. Kentenich ◽  
F. Hepp ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Cancer Patients ◽  
Antibody Therapy ◽  
Breast Cancer Patients ◽  
Metastatic Cells

Persistence of occult metastatic cells in bone marrow of breast cancer patients despite systemic adjuvant treatment

The Breast ◽  
2003 ◽  
Vol 12 ◽  
pp. S21
Author(s):  
W. Janni ◽  
B. Rack ◽  
D. Rjosk ◽  
E. Klanner ◽  
C. Kentenich ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Bone Marrow ◽  
Cancer Patients ◽  
Adjuvant Treatment ◽  
Breast Cancer Patients ◽  
Metastatic Cells
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