scholarly journals Chasing the immortal strand: evidence for nature's way of protecting the breast genome

2011 ◽  
Vol 13 (1) ◽  
Author(s):  
Nikolajs Zeps ◽  
Christine Hemmings
Keyword(s):  
Immortal Strand

scholarly journals Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro

2005 ◽  
Vol 170 (5) ◽  
pp. 721-732 ◽  
Author(s):  
Phillip Karpowicz ◽  
Cindi Morshead ◽  
Angela Kam ◽  
Eric Jervis ◽  
John Ramunas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Genetic Material ◽  
Precursor Cells ◽  
Neural Precursor Cells ◽  
Dna Templates ◽  
Cell Divisions ◽  
Immortal Strand ◽  
Asymmetric Partitioning

The immortal strand hypothesis proposes that asymmetrically dividing stem cells (SCs) selectively segregate chromosomes that bear the oldest DNA templates. We investigated cosegregation in neural stem cells (NSCs). After exposure to the thymidine analogue 5-bromo-2-deoxyuridine (BrdU), which labels newly synthesized DNA, a subset of neural precursor cells were shown to retain BrdU signal. It was confirmed that some BrdU-retaining cells divided actively, and that these cells exhibited some characteristics of SCs. This asymmetric partitioning of DNA then was demonstrated during mitosis, and these results were further supported by real time imaging of SC clones, in which older and newly synthesized DNA templates were distributed asymmetrically after DNA synthesis. We demonstrate that NSCs are unique among precursor cells in the uneven partitioning of genetic material during cell divisions.

scholarly journals The Immortal Strand Hypothesis: How Could It Work?

Cell ◽  
2008 ◽  
Vol 133 (1) ◽  
pp. 21-23 ◽  
Author(s):  
Daniel J. Lew ◽  
Daniel J. Burke ◽  
Anindya Dutta
Keyword(s):  
Immortal Strand

scholarly journals The Immortal Strand Hypothesis: Segregation and Reconstruction

Cell ◽  
2007 ◽  
Vol 129 (7) ◽  
pp. 1239-1243 ◽  
Author(s):  
Thomas A. Rando
Keyword(s):  
Immortal Strand

scholarly journals The (not so) immortal strand hypothesis

2015 ◽  
Vol 14 (2) ◽  
pp. 238-241 ◽  
Author(s):  
Cristian Tomasetti ◽  
Ivana Bozic
Keyword(s):  
Immortal Strand

scholarly journals The immortal strand hypothesis: still non-randomly segregating opinions

2012 ◽  
Vol 3 (3) ◽  
pp. 203-211 ◽  
Author(s):  
Jane A. Wakeman ◽  
Abdelkrim Hmadcha ◽  
Bernat Soria ◽  
Ramsay J. McFarlane
Keyword(s):  
Stem Cells ◽  
Cell Division ◽  
Asymmetric Cell Division ◽  
Current Data ◽  
Immortal Strand ◽  
Published Evidence ◽  
Conflicting Evidence ◽  
The Future ◽  
Dna Strands ◽  
Review Current

AbstractCairns first suggested a mechanism for protecting the genomes of stem cells (SCs) from replicative errors some 40 years ago when he proposed the immortal strand hypothesis, which argued for the inheritance of a so-called immortal strand by an SC following asymmetric SC divisions. To date, the existence of immortal strands remains contentious with published evidence arguing in favour of and against the retention of an immortal strand by asymmetrically dividing SCs. The conflicting evidence is derived from a diverse array of studies on adult SC types and is predominantly based on following the fate of labelled DNA strands during asymmetric cell division events. Here, we review current data, highlighting limitations of such labelling techniques, and suggest how interpretation of such data may be improved in the future.

scholarly journals Correction: Support for the immortal strand hypothesis: neural stem cells partition DNA asymmetrically in vitro

2005 ◽  
Vol 170 (7) ◽  
pp. 1169-1169
Author(s):  
Phillip Karpowicz ◽  
Cindi Morshead ◽  
Angela Kam ◽  
Eric Jervis ◽  
John Ramunas ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Immortal Strand

scholarly journals Cancer and the Immortal Strand Hypothesis

Genetics ◽  
2006 ◽  
Vol 174 (3) ◽  
pp. 1069-1072 ◽  
Author(s):  
John Cairns
Keyword(s):  
Immortal Strand

scholarly journals A probabilistic framework for cellular lineage reconstruction using single-cell 5-hydroxymethylcytosine sequencing

2019 ◽  
Author(s):  
Chatarin Wangsanuwat ◽  
Javier F. Aldeguer ◽  
Nicolas C. Rivron ◽  
Siddharth S. Dey
Keyword(s):  
Cell Division ◽  
Single Cell ◽  
Cell Biology ◽  
Individual Cell ◽  
Immortal Strand ◽  
Preimplantation Mouse Embryos ◽  
Significant Barrier ◽  
Lineage Tree ◽  
Preimplantation Mouse ◽  
Lineage Trees

AbstractLineage reconstruction is central to understanding tissue development and maintenance. While powerful tools to infer cellular relationships have been developed, these methods typically have a clonal resolution that prevent the reconstruction of lineage trees at an individual cell division resolution. Moreover, these methods require a transgene, which poses a significant barrier in the study of human tissues. To overcome these limitations, we report scPECLR, a probabilistic algorithm to endogenously infer lineage trees at a single cell-division resolution using 5-hydroxymethylcytosine. When applied to 8-cell preimplantation mouse embryos, scPECLR predicts the full lineage tree with greater than 95% accuracy. Further, scPECLR can accurately extract lineage information for a majority of cells when reconstructing larger trees. Finally, we show that scPECLR can also be used to map chromosome strand segregation patterns during cell division, thereby providing a strategy to test the “immortal strand” hypothesis in stem cell biology. Thus, scPECLR provides a generalized method to endogenously reconstruct lineage trees at an individual cell-division resolution.

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