scholarly journals Mammary epithelial cells immortalized by over-expression of the catalytic subunit of human telomerase may represent a model for mammary epithelial differentiation

2000 ◽  
Vol 2 (S1) ◽  
Author(s):  
J DiRenzo ◽  
R Rivera-Gonzalez ◽  
M Brown
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Epithelial Differentiation ◽  
Mammary Epithelial ◽  
Catalytic Subunit ◽  
Over Expression ◽  
Human Telomerase

scholarly journals Human telomerase prolongs the life span of bovine mammary epithelial cells

2007 ◽  
Vol 16 (Suppl. 2) ◽  
pp. 341-345
Author(s):  
W.-J. Sai ◽  
L.-N. Sui ◽  
X.-R. Peng ◽  
Y.-G. Wang ◽  
S.-Y. Peng ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Life Span ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Bovine Mammary Epithelial Cells ◽  
Human Telomerase

scholarly journals Comparison of human mammary epithelial cells immortalized by simian virus 40 T-Antigen or by the telomerase catalytic subunit

Oncogene ◽  
2002 ◽  
Vol 21 (1) ◽  
pp. 128-139 ◽  
Author(s):  
Christian D Toouli ◽  
Lily I Huschtscha ◽  
Axel A Neumann ◽  
Jane R Noble ◽  
Lorel M Colgin ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Mammary Epithelial Cells ◽  
Simian Virus 40 ◽  
Simian Virus ◽  
T Antigen ◽  
Mammary Epithelial ◽  
Catalytic Subunit ◽  
Human Mammary Epithelial Cells ◽  
Human Mammary Epithelial

scholarly journals Co-expression of transcription factor AP-2beta (TFAP2B) and GATA3 in human mammary epithelial cells with intense, apicobasal immunoreactivity for CK8/18

2021 ◽  
Author(s):  
M. Raap ◽  
L. Gierendt ◽  
C. Werlein ◽  
E. Kuehnle ◽  
H. H. Kreipe ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Hormone Receptors ◽  
Mammary Epithelial Cells ◽  
Lobular Carcinoma ◽  
Epithelial Differentiation ◽  
Mammary Epithelial ◽  
Normal Mammary Gland ◽  
Stem Cell Markers ◽  
Normal Breast Epithelium ◽  
Differentiation Marker

AbstractAP-2β is a new mammary epithelial differentiation marker and its expression is preferentially retained and enhanced in lobular carcinoma in situ and invasive lobular breast cancer. In normal breast epithelium AP-2β is expressed in a scattered subpopulation of luminal cells. So far, these cells have not been further characterized. Co-expression of AP-2β protein and luminal epithelium markers (GATA3, CK8/18), hormone receptors [estrogen receptor (ER), androgen receptor (AR)] and candidate stem cells markers (CK5/14, CD44) were assessed by double-immunofluorescence staining in normal mammary gland epithelium. The subpopulation of AP-2β-positive mammary epithelial cells showed an almost complete, superimposable co-expression with GATA3 and a peculiar intense, ring-like appearing immunoreactivity for CK8/18. Confocal immunofluorescence microscopy revealed an apicobasal staining for CK8/18 in AP-2β-positive cells, which was not seen in in AP-2β-negative cells. Furthermore, AP-2β-positive displayed a partial co-expression with ER and AR, but lacked expression of candidate stem cell markers CK5/14 and CD44. In summary, AP-2β is a new luminal mammary epithelial differentiation marker, which is expressed in the GATA3-positive subpopulation of luminal epithelial cells. These AP-2β-positive/GATA3-positive cells also show a peculiar CK8/18-expression which may indicate a previously unknown functionally specialized mammary epithelial cell population.

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