scholarly journals Breast tumors induce the recruitment of AC133+KDR+ endothelial precursor cells mobilized by plasma vascular endothelial growth factor

2005 ◽  
Vol 7 (S2) ◽  
Author(s):  
M Botelho ◽  
I Amendoeira ◽  
J Diamond ◽  
S Dias ◽  
F Schmitt
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Breast Tumors ◽  
Precursor Cells ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

scholarly journals Chemiluminescence immunoassay for vascular endothelial growth factor (vascular permeability factor) in tumor-tissue homogenates

1996 ◽  
Vol 42 (11) ◽  
pp. 1777-1784 ◽  
Author(s):  
J M Schlaeppi ◽  
U Eppenberger ◽  
G Martiny-Baron ◽  
W Küng
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Total Protein ◽  
Breast Tumors ◽  
Cross Reactivity ◽  
Vascular Endothelial ◽  
Vascular Permeability Factor ◽  
Ovarian Carcinomas ◽  
Chemiluminescence Immunoassay ◽  
Endothelial Growth Factor

Abstract We developed a two-site chemiluminescence immunoassay for human vascular endothelial growth factor (VEGF). The assay recognized both VEGF121 and VEGF165 isoforms, but had no detectable cross-reactivity with platelet-derived growth factor or placenta growth factor. The range of detection was between 30 ng/L and 30 micrograms/L VEGF. Inter- and intraassay variations were 8.2-8.3% and 7.2-7.6%, respectively. VEGF concentrations were measured in the cytosolic extracts of 45 ovarian and 142 primary breast tumors. The amount of VEGF in the ovarian tumors (median = 0.46 ng/mg total protein, range 0-15.8 ng/mg) was significantly (P = 0.03) higher compared with the breast tumors (median = 0.24 ng/mg total protein, range 0-12.3 ng/mg). In 32 and 7 extracts of normal breast tissues adjacent and distant to the tumors, respectively, VEGF concentrations were significantly much lower (P < 0.0001). The detection of substantial amounts of VEGF in two invasive tumors (compared with normal tissues) suggests that the assay should be a useful tool for investigating the prognostic value of VEGF in breast and ovarian carcinomas and for selecting patients for future anti-VEGF therapy.

Quantitation of Vascular Endothelial Growth Factor mRNA Levels in Human Breast Tumors and Metastatic Lymph Nodes

1997 ◽  
Vol 64 (1) ◽  
pp. 41-51 ◽  
Author(s):  
Paul S. Wright ◽  
David E. Loudy ◽  
Doreen E. Cross-Doersen ◽  
Lauren R. Montgomery ◽  
Jean Sprinkle-Cavallo ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Lymph Nodes ◽  
Breast Tumors ◽  
Mrna Levels ◽  
Vascular Endothelial ◽  
Human Breast ◽  
Metastatic Lymph Nodes ◽  
Metastatic Lymph ◽  
Endothelial Growth Factor
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