scholarly journals Alternative initiation and splicing in dicer gene expression in human breast cells

2005 ◽  
Vol 7 (4) ◽  
Author(s):  
Charletha V Irvin-Wilson ◽  
Gautam Chaudhuri
Keyword(s):  
Gene Expression ◽  
Human Breast ◽  
Breast Cells

scholarly journals Comparative activity of pulsed or continuous estradiol exposure on gene expression and proliferation of normal and tumoral human breast cells

2002 ◽  
Vol 28 (3) ◽  
pp. 165-175 ◽  
Author(s):  
V Cavailles ◽  
A Gompel ◽  
MC Portois ◽  
S Thenot ◽  
N Mabon ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Lines ◽  
Hormone Replacement ◽  
Primary Cultures ◽  
Continuous Treatment ◽  
Luciferase Reporter ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Kinetic Profile ◽  
Breast Cells

Intranasal administration of hormone replacement therapy presents an original plasma kinetic profile with transient estrogen levels giving rise to the concept of pulsed therapy. To further understand the molecular effects of this new therapy, we have compared the effects of pulsed and continuous estradiol treatments on two critical aspects of estradiol action: gene expression and cell proliferation. Cells were stimulated with estradiol as 1-h pulsed or 24-h continuous treatments at concentrations such that the 24-h exposure (concentration x time) was identical in both conditions. In MCF7 cells, the transcriptional activity of estrogen receptors (ER) on a transiently transfected responsive estrogen response element-luciferase reporter construct was shown to be drastically (approximately 10-fold) and similarly stimulated after both treatments. Moreover, the increased mRNA expression of three representative estradiol-sensitive genes (pS2, cathepsin D, progesterone receptor), evaluated by Northern blot, was identical after 1-h pulse with 7 nM estradiol or continuous treatment with 0.29 nM estradiol with the same kinetic profile over 48 h. Proliferation was quantified by a histomorphometric method on primary cultures of human normal breast cells from reduction mammoplasties and using a fluorescence DNA assay in six human breast cancer cell lines which were ER positive or negative. After a 7-day treatment period, estradiol had no effect on the proliferation of the three ER negative cell lines (BT20, MDA MB231, SK BR3) but significantly stimulated the proliferation of the normal cells and of the three tumoral hormone-sensitive cell lines (MCF7, T47D, ZR 75-1); both hormone treatments producing the same increases in cell growth. In conclusion, we have shown that the genomic or proliferative effects of estradiol were identical with pulsed or continuous treatments, thus indicating that estrogenic effects are not strictly related to concentrations but rather to total hormone exposure.

The effects of lycopene on the proliferation of human breast cells and BRCA1 and BRCA2 gene expression

2004 ◽  
Vol 40 (11) ◽  
pp. 1768-1775 ◽  
Author(s):  
N Chalabi ◽  
L Le Corre ◽  
J-C Maurizis ◽  
Y-J Bignon ◽  
D.J Bernard-Gallon
Keyword(s):  
Gene Expression ◽  
Brca2 Gene ◽  
Human Breast ◽  
Brca1 And Brca2 ◽  
Breast Cells

scholarly journals Identification of a GABPα/β Binding Site Involved in the Induction of Oxytocin Receptor Gene Expression in Human Breast Cells. Potentiation by c-Fos/c-Jun*

Endocrinology ◽  
1999 ◽  
Vol 140 (5) ◽  
pp. 2268-2279 ◽  
Author(s):  
Sarasija Hoare ◽  
John A. Copland ◽  
Thomas G. Wood ◽  
Yow-Jiun Jeng ◽  
Michael G. Izban ◽  
...  
Keyword(s):  
Gene Expression ◽  
Binding Site ◽  
Receptor Gene ◽  
Oxytocin Receptor ◽  
Human Breast ◽  
Oxytocin Receptor Gene ◽  
Breast Cells ◽  
Receptor Gene Expression
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