scholarly journals Notochordal cell disappearance and modes of apoptotic cell death in a rat tail static compression-induced disc degeneration model

2014 ◽  
Vol 16 (1) ◽  
pp. R31 ◽  
Author(s):  
Takashi Yurube ◽  
Hiroaki Hirata ◽  
Kenichiro Kakutani ◽  
Koichiro Maeno ◽  
Toru Takada ◽  
...  
Keyword(s):  
Cell Death ◽  
Disc Degeneration ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Static Compression ◽  
Notochordal Cell ◽  
Rat Tail

scholarly journals Involvement of Autophagy in Rat Tail Static Compression-Induced Intervertebral Disc Degeneration and Notochordal Cell Disappearance

2021 ◽  
Vol 22 (11) ◽  
pp. 5648
Author(s):  
Takashi Yurube ◽  
Hiroaki Hirata ◽  
Masaaki Ito ◽  
Yoshiki Terashima ◽  
Yuji Kakiuchi ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Time Course ◽  
The Body ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Autophagic Flux ◽  
Static Compression ◽  
Notochordal Cell ◽  
Notochordal Cells ◽  
Rat Tail

The intervertebral disc is the largest avascular low-nutrient organ in the body. Thus, resident cells may utilize autophagy, a stress-response survival mechanism, by self-digesting and recycling damaged components. Our objective was to elucidate the involvement of autophagy in rat experimental disc degeneration. In vitro, the comparison between human and rat disc nucleus pulposus (NP) and annulus fibrosus (AF) cells found increased autophagic flux under serum deprivation rather in humans than in rats and in NP cells than in AF cells of rats (n = 6). In vivo, time-course Western blotting showed more distinct basal autophagy in rat tail disc NP tissues than in AF tissues; however, both decreased under sustained static compression (n = 24). Then, immunohistochemistry displayed abundant autophagy-related protein expression in large vacuolated disc NP notochordal cells of sham rats. Under temporary static compression (n = 18), multi-color immunofluorescence further identified rapidly decreased brachyury-positive notochordal cells with robust expression of autophagic microtubule-associated protein 1 light chain 3 (LC3) and transiently increased brachyury-negative non-notochordal cells with weaker LC3 expression. Notably, terminal deoxynucleotidyl transferase dUTP nick end labeling-positive apoptotic death was predominant in brachyury-negative non-notochordal cells. Based on the observed notochordal cell autophagy impairment and non-notochordal cell apoptosis induction under unphysiological mechanical loading, further investigation is warranted to clarify possible autophagy-induced protection against notochordal cell disappearance, the earliest sign of disc degeneration, through limiting apoptosis.

KIOM-79 prevents apoptotic cell death and AGEs accumulation in the retina of diabetic db/db mice

Planta Medica ◽  
2007 ◽  
Vol 73 (09) ◽  
Author(s):  
YS Kim ◽  
EJ Sohn ◽  
HY Lee ◽  
CS Kim ◽  
YM Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

Apoptotic cell death triggered by nitric oxide in pancreatic beta-cells

Diabetes ◽  
1995 ◽  
Vol 44 (7) ◽  
pp. 733-738 ◽  
Author(s):  
H. Kaneto ◽  
J. Fujii ◽  
H. G. Seo ◽  
K. Suzuki ◽  
T. Matsuoka ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Cell Death ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death
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