scholarly journals Proteomic profiling and functional characterization of early and late shoulder osteoarthritis

2013 ◽  
Vol 15 (6) ◽  
pp. R180 ◽  
Author(s):  
John Wanner ◽  
Roopashree Subbaiah ◽  
Yelenna Skomorovska-Prokvolit ◽  
Yousef Shishani ◽  
Eric Boilard ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Proteomic Profiling ◽  
Shoulder Osteoarthritis

Proteomic Profiling and Functional Characterization of Multiple Post-Translational Modifications of Tubulin

2015 ◽  
Vol 14 (8) ◽  
pp. 3292-3304 ◽  
Author(s):  
Ningning Liu ◽  
Yun Xiong ◽  
Yiran Ren ◽  
Linlin Zhang ◽  
Xianfei He ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Proteomic Profiling ◽  
Post Translational Modifications

Proteomic profiling between CNE-2 and its strongly metastatic subclone S-18 and functional characterization of HSP27 in metastasis of nasopharyngeal carcinoma

PROTEOMICS ◽  
2011 ◽  
Vol 11 (14) ◽  
pp. 2911-2920 ◽  
Author(s):  
Guo-Ping Li ◽  
Hua Wang ◽  
Yiu-Kay Lai ◽  
Shao-Chun Chen ◽  
Marie C. M. Lin ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Functional Characterization ◽  
Proteomic Profiling

scholarly journals Quantitative proteomic analysis and functional characterization of Acanthamoeba castellanii exosome-like vesicles

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Wei-Chen Lin ◽  
Chia-Yun Tsai ◽  
Jian-Ming Huang ◽  
Shang-Rung Wu ◽  
Lichieh Julie Chu ◽  
...  
Keyword(s):  
Immune Response ◽  
Proteomic Analysis ◽  
Functional Characterization ◽  
Acanthamoeba Castellanii ◽  
Host Cells ◽  
C6 Cells ◽  
Proteomic Profiling ◽  
Quantitative Proteomic Analysis ◽  
Rat Glioma

Abstract Background Pathogenic protozoans use extracellular vesicles (EVs) for intercellular communication and host manipulation. Acanthamoeba castellanii is a free-living protozoan that may cause severe keratitis and fatal granulomatous encephalitis. Although several secreted molecules have been shown to play crucial roles in the pathogenesis of Acanthamoeba, the functions and components of parasite-derived EVs are far from understood. Methods Purified EVs from A. castellanii were confirmed by electron microscopy and nanoparticle tracking analysis. The functional roles of parasite-derived EVs in the cytotoxicity to and immune response of host cells were examined. The protein composition in EVs from A. castellanii was identified and quantified by LC-MS/MS analysis. Results EVs from A. castellanii fused with rat glioma C6 cells. The parasite-derived EVs induced an immune response from human THP-1 cells and a cytotoxic effect in C6 cells. Quantitative proteomic analysis identified a total of 130 proteins in EVs. Among the identified proteins, hydrolases (50.2%) and oxidoreductases (31.7%) were the largest protein families in EVs. Furthermore, aminopeptidase activities were confirmed in EVs from A. castellanii. Conclusions The proteomic profiling and functional characterization of EVs from A. castellanii provide an in-depth understanding of the molecules packaged into EVs and their potential mechanisms mediating the pathogenesis of this parasite.

Comparative proteomic profiling and functional characterization of venom pooled from captive Crotalus durissus terrificus specimens and the Brazilian crotalic reference venom

Toxicon ◽  
2020 ◽  
Vol 185 ◽  
pp. 26-35
Author(s):  
Lidia J. Tasima ◽  
Daniela M. Hatakeyama ◽  
Caroline Serino-Silva ◽  
Caroline F.B. Rodrigues ◽  
Eduardo O.V. de Lima ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Proteomic Profiling ◽  
Crotalus Durissus Terrificus ◽  
Crotalus Durissus

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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