scholarly journals Flow cytometric characterization of freshly isolated and culture expanded human synovial cell populations in patients with chronic arthritis

2010 ◽  
Vol 12 (1) ◽  
pp. R15 ◽  
Author(s):  
Kristel B Van Landuyt ◽  
Elena A Jones ◽  
Dennis McGonagle ◽  
Frank P Luyten ◽  
Rik J Lories
Keyword(s):  
Synovial Cell ◽  
Chronic Arthritis ◽  
Cell Populations ◽  
Flow Cytometric ◽  
Human Synovial Cell

scholarly journals Isolation and Characterization of Highly Pure Type A Spermatogonia From Sterlet (Acipenser ruthenus) Using Flow-Cytometric Cell Sorting

2021 ◽  
Vol 9 ◽  
Author(s):  
Xuan Xie ◽  
Tomáš Tichopád ◽  
Galina Kislik ◽  
Lucie Langerová ◽  
Pavel Abaffy ◽  
...  
Keyword(s):  
Germ Cells ◽  
Cell Sorting ◽  
Developmental Stages ◽  
Type A ◽  
Cell Populations ◽  
Forward Scatter ◽  
Flow Cytometric ◽  
Flow Cytometric Cell ◽  
Type A Spermatogonia

Sturgeons are among the most ancient linages of actinopterygians. At present, many sturgeon species are critically endangered. Surrogate production could be used as an affordable and a time-efficient method for endangered sturgeons. Our study established a method for identifying and isolating type A spermatogonia from different developmental stages of testes using flow cytometric cell sorting (FCM). Flow cytometric analysis of a whole testicular cell suspension showed several well-distinguished cell populations formed according to different values of light scatter parameters. FCM of these different cell populations was performed directly on glass slides for further immunocytochemistry to identify germ cells. Results showed that the cell population in gate P1 on a flow cytometry plot (with high forward scatter and high side scatter parameter values) contains the highest amount of type A spermatogonia. The sorted cell populations were characterized by expression profiles of 10 germ cell specific genes. The result confirmed that setting up for the P1 gate could precisely sort type A spermatogonia in all tested testicular developmental stages. The P2 gate, which was with lower forward scatter and side scatter values mostly, contained type B spermatogonia at a later maturing stage. Moreover, expressions of plzf, dnd, boule, and kitr were significantly higher in type A spermatogonia than in later developed germ cells. In addition, plzf was firstly found as a reliable marker to identify type A spermatogonia, which filled the gap of identification of spermatogonial stem cells in sterlet. It is expected to increase the efficiency of germ stem cell culture and transplantation with plzf identification. Our study thus first addressed a phenotypic characterization of a pure type A spermatogonia population in sterlet. FCM strategy can improve the production of sturgeons with surrogate broodstock and further the analysis of the cellular and molecular mechanisms of sturgeon germ cell development.

scholarly journals Human synovial cells secrete a 39 kDa protein similar to a bovine mammary protein expressed during the non-lactating period

1990 ◽  
Vol 269 (1) ◽  
pp. 265-268 ◽  
Author(s):  
P Nyirkos ◽  
E E Golds
Keyword(s):  
Synovial Cell ◽  
Lung Fibroblasts ◽  
Synovial Cells ◽  
Terminal Sequence ◽  
Reverse Phase ◽  
Cell Monolayers ◽  
Human Synovial Cell ◽  
Sds Page

By SDS/PAGE analysis we have observed that human synovial cell monolayers secrete a prominent 39 kDa protein which could not be detected in skin and lung fibroblasts. This protein was purified to homogeneity by heparin-Sepharose chromatography and reverse-phase h.p.l.c. The N-terminal sequence was found to be almost identical to that of a recently described bovine protein detected in the mammary secretions during the involutionary phase of the lactational cycle. Characterization of this 39 kDa protein may provide a useful marker for classification of connective tissue cells.

Characterization of a human synovial cell antigen: VCAM‐1 and inflammatory arthritis

2001 ◽  
Vol 79 (5) ◽  
pp. 419-428 ◽  
Author(s):  
RA Carter ◽  
K O'Donnell ◽  
S Sachthep ◽  
F Cicuttini ◽  
AW Boyd ◽  
...  
Keyword(s):  
Inflammatory Arthritis ◽  
Synovial Cell ◽  
Cell Antigen ◽  
Human Synovial Cell

scholarly journals Characterization of the Myeloid Cell Populations’ Resident in the Porcine Palatine Tonsil

2018 ◽  
Vol 9 ◽  
Author(s):  
Ferran Soldevila ◽  
Jane C. Edwards ◽  
Simon P. Graham ◽  
Lisa M. Stevens ◽  
Bentley Crudgington ◽  
...  
Keyword(s):  
Myeloid Cell ◽  
Palatine Tonsil ◽  
Cell Populations

Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System

2012 ◽  
Vol 21 (9) ◽  
pp. 2021-2032 ◽  
Author(s):  
Silvia Claros ◽  
Noela Rodríguez-Losada ◽  
Encarnación Cruz ◽  
Enrique Guerado ◽  
José Becerra ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Culture System ◽  
Progenitor Cell ◽  
Three Dimensional ◽  
Collagen Gel ◽  
Cell Populations ◽  
Collagen Gel Culture ◽  
Gel Culture

Flow cytometric quantitation and characterization of the T-lymphocyte memory response to CMV in healthy donors

Cytotherapy ◽  
2002 ◽  
Vol 4 (1) ◽  
pp. 29-40 ◽  
Author(s):  
N. Hensel ◽  
J.J. Melenhorst ◽  
K. Bradstock ◽  
A.P. Schwarer ◽  
R. Eniafe ◽  
...  
Keyword(s):  
T Lymphocyte ◽  
Memory Response ◽  
Flow Cytometric ◽  
Healthy Donors

scholarly journals Interleukin 4 increases human synovial cell expression of VCAM-1 and T cell binding.

1994 ◽  
Vol 53 (9) ◽  
pp. 601-607 ◽  
Author(s):  
H Shimada ◽  
K Eguchi ◽  
Y Ueki ◽  
M Nakashima ◽  
I Yamashita ◽  
...  
Keyword(s):  
T Cell ◽  
Synovial Cell ◽  
Interleukin 4 ◽  
Cell Binding ◽  
Human Synovial Cell ◽  
Cell Expression
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