scholarly journals Gene expression profiling in the synovium identifies a predictive signature of absence of response to adalimumab therapy in rheumatoid arthritis

2009 ◽  
Vol 11 (2) ◽  
pp. R57 ◽  
Author(s):  
Valérie Badot ◽  
Christine Galant ◽  
Adrien Nzeusseu Toukap ◽  
Ivan Theate ◽  
Anne-Lise Maudoux ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Adalimumab Therapy

scholarly journals Peripheral blood gene expression profiling in rheumatoid arthritis

2005 ◽  
Vol 6 (5) ◽  
pp. 388-397 ◽  
Author(s):  
F M Batliwalla ◽  
E C Baechler ◽  
X Xiao ◽  
W Li ◽  
S Balasubramanian ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Gene Expression Profiling ◽  
Peripheral Blood ◽  
Expression Profiling ◽  
Blood Gene Expression ◽  
Peripheral Blood Gene Expression

Blood cell gene expression profiling in rheumatoid arthritis

2004 ◽  
Vol 93 (2-3) ◽  
pp. 217-226 ◽  
Author(s):  
Lone Frier Bovin ◽  
Klaus Rieneck ◽  
Christopher Workman ◽  
Henrik Nielsen ◽  
Søren Freiesleben Sørensen ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Blood Cell ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Cell Gene Expression ◽  
Cell Gene

scholarly journals Gene Expression Profiling of Rheumatoid Arthritis Synovial Cells Treated with Antirheumatic Drugs

2007 ◽  
Vol 12 (3) ◽  
pp. 328-340 ◽  
Author(s):  
Thomas Häupl ◽  
Mehdi Yahyawi ◽  
Carsten Lübke ◽  
Jochen Ringe ◽  
Thorsten Rohrlach ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profile ◽  
Expression Profiling ◽  
Synovial Fibroblasts ◽  
Mechanisms Of Action ◽  
Antirheumatic Drugs ◽  
Polymerase Chain ◽  
High Doses

Nonbiological therapeutics are frequently used for the treatment of patients with rheumatoid arthritis (RA). Because the mechanisms of action of these therapeutics are unclear, the authors aimed to elucidate the molecular effects of typical antirheumatic drugs on the expression profile of RA-related genes expressed in activated synovial fibroblasts. For reasons of standardization and comparability, immortalized synovial fibroblasts derived from RA (RASF) and normal donors (NDSF) were treated with methotrexate, prednisolone, or diclofenac and used for gene expression profiling with oligonucleotide microarrays. The cytotoxicity of the antirheumatic drugs was tested in different concentrations by MTS tetrazolium assay. Genes that were differentially expressed in RASF compared to NDSF and reverted by treatment with antirheumatic drugs were verified by semiquantitative polymerase chain reaction and by chemiluminescent enzyme immunoassay. Treatment with methotrexate resulted in the reversion of the RA-related expression profile of genes associated with growth and apoptosis including insulin-like growth factor binding protein 3, retinoic acid induced 3, and caveolin 2 as well as in the re-expression of the cell adhesion molecule integrin α6. Prednisolone reverted the RA-related profile of genes that are known from inflammation and suppressed interleukins 1β and 8. Low or high doses of diclofenac had no effect on the expression profile of genes related to RA in synovial fibroblasts. These data give the first insight into the mechanisms of action of common antirheumatic drugs used for the treatment of arthritides. Synovial fibroblasts reflect the disease-related pathophysiology and are useful tools for screening putative antirheumatic compounds. ( Journal of Biomolecular Screening 2007:328-340)

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