scholarly journals Detection of bacterial components in synovial tissue from patients with inflammatory arthritis by using PCR with pan bacterial 23S rRNA and 16S rRNA primers, and gas chromatography-mass spectrometry

10.1186/ar237 ◽  
2001 ◽  
Vol 3 (S2) ◽  
Author(s):  
T Chen ◽  
M Rimpiläinen ◽  
R Luukkainen ◽  
T Möttönen ◽  
T Yli-Jama ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
16S Rrna ◽  
Synovial Tissue ◽  
Inflammatory Arthritis ◽  
Gas Chromatography Mass Spectrometry ◽  
23S Rrna ◽  
Chromatography Mass Spectrometry

scholarly journals Different metabolic pathways involved in anthracene biodegradation by Brevibacillus, Pseudomonas and Methylocystis Species

2022 ◽  
Vol 11 (1) ◽  
Author(s):  
Mariam M. Magdy ◽  
Yasser Gaber ◽  
Mohamed Sebak ◽  
Ahmed F. Azmy ◽  
Sameh AbdelGhani
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
16S Rrna ◽  
Pcr Amplification ◽  
Cost Effective ◽  
Gas Chromatography Mass Spectrometry ◽  
Bacterial Isolates ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Genes Encoding

Abstract Background Polycyclic aromatic hydrocarbons (PAHs) such as anthracene are one of the most toxic contaminants to our environment. Microbial biodegradation of these xenobiotics is a cost-effective technological solution. The present study aimed to recover some bacterial isolates from Beni-Suef Governorate in Egypt with high capabilities of anthracene biodegradation. The selected isolates were molecularly characterized by 16S rRNA gene sequencing, the degree of anthracene biodegradation was monitored using optical density (OD) and high-performance liquid chromatography (HPLC), PCR amplification of some selected genes encoding biodegradation of PAHs was monitored, and gas chromatography–mass spectrometry (GC–MS) analysis was applied for detecting the resulted metabolites. Result Three bacterial isolates were studied, the 16s rRNA sequences of the isolates showed homology of the first isolate to Brevibacillus sp. (94.58 %), the second isolates showed homology to Pseudomonas sp. (94.53%) and the third isolate showed homology to Methylocystis sp. (99.61 %), all isolates showed the ability to degrade anthracene. PCR amplification of some selected genes encoding biodegradation of PAHs revealed the presence of many biodegrading genes in the selected strains. Gas chromatography-mass spectrometry (GC–MS) analysis of the metabolites resulted from anthracene biodegradation in the present study suggested that more than one biodegradation pathway was followed by the selected isolates. Conclusions The selected strains could represent a potential bioremediation tool in solving the PAHs problem in the Egyptian environment with a clean and cost-effective technique. Graphical Abstract

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