Different Techniques of Residual Composite Removal following Debonding—Time Taken and Surface Enamel Appearance

1992 ◽  
Vol 19 (2) ◽  
pp. 131-137 ◽  
Author(s):  
R. G. Oliver ◽  
J. Griffiths
Keyword(s):  
Tungsten Carbide ◽  
Cost Effective ◽  
Surface Damage ◽  
Enamel Surface ◽  
Practical Skills ◽  
Surface Appearance ◽  
Significant Difference ◽  
Scanning Electron

Four different methods of in vitro residual composite removal following debonding performed by two different operators (an orthodontist and a hygienist) were assessed for enamel surface damage (using scanning electron microscopy) and the time involved. There was no difference in the quality of enamel surface appearance between the two operators, regardless of the method used for composite removal. There was a statistically significant difference (P < 0·05) for the time taken for composite removal between the two operators using a tungsten carbide bur method only. It is suggested that an expanded duties auxiliary with the practical skills of the hygienist would be able to remove residual composite debris and produce a satisfactory polished enamel surface using tungsten carbide burs and aluminium oxide polishing discs, thus becoming a safe and cost-effective member of the orthodontic team.

scholarly journals Evaluation of Enamel Surface after Removal of Adhesive Resin: An in Vitro Study

2018 ◽  
Vol 8 (2) ◽  
pp. 12-16
Author(s):  
Geeta Pyakurel ◽  
Gazi Shamim Hassan ◽  
Mahmood Sajedeen
Keyword(s):  
Surface Roughness ◽  
Stainless Steel ◽  
Tungsten Carbide ◽  
In Vitro Study ◽  
T Test ◽  
Enamel Surface ◽  
Adhesive Resin ◽  
Significant Difference ◽  
And Tungsten

Introduction: Preservation of the enamel surface during the removal of orthodontic appliance is an essential aspect for clinicians. However, various therapeutic measures can affect the enamel surface. The objective of the research is to evaluate the roughness of enamel surface after the removal of adhesive resin. Materials & Method: 40 extracted human premolar teeth were randomly divided into two groups and the enamel surfaces were initially subjected to profilometer for the assessment of surface roughness. Following bracket bonding, debonding was done and adhesive resin was removed by stainless steel bur in Group A and tungsten carbide bur in Group B. Again, the surface roughness was measured by profilometer. Independent t-test was performed to compare the enamel surface roughness between two groups and paired t-test to compare the enamel surface roughness within the groups. Result: The mean average surface roughness of stainless steel bur was 27.009 ± 4.8420 μm and tungsten carbide was 31.426 ± 5.0956 μm. The result showed that there was significant difference in enamel surface roughness between two groups. Conclusion: The roughness values were found to be significantly decreased with the use of stainless steel bur (SS) than tungsten carbide bur (TC).

In vitro Evaluation of Enamel Surface Treated with Fluoride After Bleaching and Etching Erosive Processes

2018 ◽  
Vol 69 (7) ◽  
pp. 1714-1717
Author(s):  
Roxana Ionela Vasluianu ◽  
Norina Consuela Forna ◽  
Elena Raluca Baciu ◽  
Mirela Zaltariov ◽  
Lavinia Vasiliu ◽  
...  
Keyword(s):  
Enamel Surface ◽  
Spectroscopy Analysis ◽  
X Ray ◽  
Fluoride Treatment ◽  
Enamel Structure ◽  
Erosion Effect ◽  
Enamel Composition ◽  
Morphology Changes ◽  
Scanning Electron

The anti-erosion effect of fluoride on the enamel surface was investigated by ATR-FTIR, SEM and EDX techniques. Four extracted teeth (two incisors and two premolars) were initially bleached with carabamide peroxide and etched with ortho-phosphoric acid then fluoride treatment was applied. Significant differences in enamel composition and morphology were observed providing the effect of fluoride application in remineralization of teeth. Infrared spectroscopy was employed to probe the changes in enamel structure. Scanning electron microscopy/energy dispersive X-ray spectroscopy analysis revealed higher content in F of teeth enamel. Morphology changes revealed a re-mineralization of enamel surface after the treatment with fluoride gel.

scholarly journals Scanning electron microscopic examination of enamel surface after fixed orthodontic treatment: In-vivo study

2012 ◽  
Vol 140 (1-2) ◽  
pp. 22-28 ◽  
Author(s):  
Tijana Sessa ◽  
Jelena Civovic ◽  
Tina Pajevic ◽  
Jovana Juloski ◽  
Milos Beloica ◽  
...  
Keyword(s):  
Electron Microscopic Examination ◽  
Surface Damage ◽  
Multivariate Regression Analysis ◽  
Enamel Surface ◽  
Orthodontic Appliances ◽  
Electron Microscopic ◽  
Fixed Orthodontic Appliances ◽  
Tooth Surfaces ◽  
Scanning Electron

Introduction. Therapy with fixed orthodontic appliances starts with bracket bonding and ends with debonding of brackets, leaving enamel surface varied. Objective. The aim of this pilot study was to examine enamel surface before and after debonding of orthodontic brackets by the use of scanning electron microscopy (SEM). Methods. Epoxy replicas of four patients? premolars indicated for therapy with fixed orthodontic appliances were made and brackets were bonded to their teeth with a different adhesives (Enlight, No-mix, Fuji Ortho LC and Heliosit Orthodontic) (n=4). Two months later, brackets on premolars were debonded and amounts of adhesive left on the tooth surfaces and the bracket bases were evaluated with the adhesive remnant index (ARI). After resin removal, epoxy replicas were made and the surface of premolars was evaluated with the enamel surface index (ESI). All replicas of premolars (n=32) were prepared for SEM examination and compared under different magnifications. Tooth damage was estimated based on correlation between ARItooth and ESI. Results. Pearson?s ?2 test showed no significant differences between ARItooth and ARIbracket of four materials used. Nonparametric correlations showed significant differences between ARItooth and ARIbracket, ESI and ARItooth, and between ESI and ARIbracket. Increasing of ARItooth is followed with the descent of ARIbracket and the ascent of ESI. Multivariate regression analysis showed a significant correlation between ESI and ARItooth. Conclusion. Most bond failures took place at enamel-adhesive interface. ARItooth was a predictor to enamel surface damage. The type of material did not affect enamel surface damage.

scholarly journals Preclinical and clinical validation of a novel injected molded swab for molecular assay detection of SARS-CoV-2 virus

2021 ◽  
Author(s):  
Chiara Elia Ghezzi ◽  
Devon R Hartigan ◽  
Justin Hardick ◽  
Rebecca Gore ◽  
Miryam Adelfio ◽  
...  
Keyword(s):  
Health Care Providers ◽  
Medical Devices ◽  
Clinical Studies ◽  
Nasal Swab ◽  
Cost Effective ◽  
Slight Reduction ◽  
Care Providers ◽  
Significant Difference ◽  
Injection Molded

During the COVID-19 public health emergency, many actions have been undertaken to help ensure that patients and health care providers had timely and continued access to high-quality medical devices to respond effectively. The development and validation of new testing supplies and equipment, including collection swab, help expand the availability and capability for various diagnostic, therapeutic, and protective medical devices in high demand during the COVID-19 emergency. Here, we report the validation of a new injection-molded anterior nasal swab, ClearTip™, that was experimentally validated in a laboratory setting as well as in independent clinical studies in comparison to gold standard flocked swabs. We have also developed an in vitro anterior nasal tissue model, that offers an efficient and clinically relevant validation tool to replicate with high fidelity the clinical swabbing workflow, while being accessible, safe, reproducible, time and cost effective. ClearTi™ displayed a greater efficiency of release of inactivated virus in the benchtop model, confirmed by greater ability to report positive samples in a clinical study in comparison to flocked swabs. We also quantified in multi-center pre-clinical and clinical studies the detection of biological materials, as proxy for viral material, that showed a statistically significant difference in one study and a slight reduction in performance in comparison to flocked swabs. Taken together these results underscore the compelling benefits of non-absorbent injected molded anterior nasal swab for COVID-19 detection, comparable to standard flocked swabs. Injection-molded swabs, as ClearTip™, could have the potential to support future swab shortage, due to its manufacturing advantages, while offering benefits in comparison to highly absorbent swabs in terms comfort, limited volume collection, and potential multiple usage.

scholarly journals Preclinical Validation of a Novel Injection-Molded Swab for the Molecular Assay Detection of SARS-CoV-2

Diagnostics ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 206
Author(s):  
Chiara E. Ghezzi ◽  
Devon R. Hartigan ◽  
Justin P. Hardick ◽  
Rebecca Gore ◽  
Miryam Adelfio ◽  
...  
Keyword(s):  
Health Care Providers ◽  
Medical Devices ◽  
Clinical Studies ◽  
Cost Effective ◽  
Care Providers ◽  
Nasal Tissue ◽  
Significant Difference ◽  
Injection Molded ◽  
Development And Validation

During the COVID-19 public health emergency, many actions have been undertaken to help ensure that patients and health care providers have timely and continued access to high-quality medical devices to respond effectively. The development and validation of new testing supplies and equipment, including collection swabs, has helped to expand the availability and capability for various diagnostic, therapeutic, and protective medical devices in high demand during the COVID-19 emergency. Here, we report the initial validation of a new injection-molded anterior nasal swab, ClearTip™, that was experimentally validated in a laboratory setting as well as in independent clinical studies in comparison to gold standard flocked swabs. We have also developed an in vitro anterior nasal tissue model which offers a novel, efficient, and clinically relevant validation tool to replicate the clinical swabbing workflow with high fidelity, while being accessible, safe, reproducible, and time- and cost-effective. ClearTip™ displayed greater inactivated virus release in the benchtop model, confirmed by its greater ability to report positive samples in a small clinical study in comparison to flocked swabs. We also quantified the detection of biological materials, as a proxy for viral material, in multi-center pre-clinical and clinical studies which showed a statistically significant difference in one study and a reduction in performance in comparison to flocked swabs. Taken together, these results emphasize the compelling benefits of non-absorbent injection-molded anterior nasal swabs for COVID-19 detection, comparable to standard flocked swabs. Injection-molded swabs, as ClearTip™, could have the potential to support future swab shortages, due to its manufacturing advantages, while offering benefits in comparison to highly absorbent swabs in terms of comfort, limited volume collection, and potential multiple usage.

scholarly journals Comparison of the Enamel Surface Roughness from Different Polishing Methods: Scanning Electron Microscopy and Atomic Force Microscopy Investigation

2020 ◽  
Vol 14 (02) ◽  
pp. 299-305
Author(s):  
Kiatanan Sugsompian ◽  
Ratchawan Tansalarak ◽  
Thosapol Piyapattamin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Surface Roughness ◽  
Atomic Force Microscopy ◽  
Enamel Surface ◽  
Similar Data ◽  
Force Microscopy ◽  
Atomic Force ◽  
Significant Difference ◽  
Scanning Electron

Abstract Objective This study aimed to compare the enamel surface roughness created by four polishing methods after debonding, by using scanning electron microscopy (SEM) and atomic force microscopy (AFM). Materials and Methods Four experimental polishing groups (Sof-Lex disc, SD; sandblaster, SB; tungsten carbide bur, TB; and white stone bur, WB) and one control group were selected from 100 premolars (n = 20/group). The experimental teeth were bonded with a bracket, thermocycled, and debonded. Residual adhesive was removed by either of the respective methods. Pre and postdebonding root mean square (Rq) values were obtained from AFM evaluations. All specimens were examined and evaluated with SEM using a modified enamel surface index (modified ESI). Statistical Analysis Differences among the polishing methods were compared with analysis of variance and Fisher’s least significant difference test at p < 0.05. Results Both microscopic evaluations indicated that the surface with the greatest roughness herein belonged to the SD group, followed by that for SB, TB, and WB groups. AFM measurements indicated a maximum postdebonding Rq herein for the WB group and a significantly greater surface roughness for the TB and WB groups than for the SD and SB groups. Among the experimental groups, SEM followed by modified ESI evaluations revealed similar data to those obtained with AFM. Significant differences were seen among all paired groups, except for that between the SB and TB groups. Conclusion Within the limitations of this study, all four polishing methods were concluded to be clinically acceptable for removing residual orthodontic adhesives.

scholarly journals The biological seal of the implant–soft tissue interface evaluated in a tissue-engineered oral mucosal model

2012 ◽  
Vol 9 (77) ◽  
pp. 3528-3538 ◽  
Author(s):  
Wen L. Chai ◽  
Ian M. Brook ◽  
Anders Palmquist ◽  
Richard van Noort ◽  
Keyvan Moharamzadeh
Keyword(s):  
Soft Tissue ◽  
Cell Attachment ◽  
Tritiated Water ◽  
Three Dimensional ◽  
Permeability Test ◽  
Significant Difference ◽  
Surface Topographies ◽  
Tissue Interface

For dental implants, it is vital that an initial soft tissue seal is achieved as this helps to stabilize and preserve the peri-implant tissues during the restorative stages following placement. The study of the implant–soft tissue interface is usually undertaken in animal models. We have developed an in vitro three-dimensional tissue-engineered oral mucosal model (3D OMM), which lends itself to the study of the implant–soft tissue interface as it has been shown that cells from the three-dimensional OMM attach onto titanium (Ti) surfaces forming a biological seal (BS). This study compares the quality of the BS achieved using the three-dimensional OMM for four types of Ti surfaces: polished, machined, sandblasted and anodized (TiUnite). The BS was evaluated quantitatively by permeability and cell attachment tests. Tritiated water (HTO) was used as the tracing agent for the permeability test. At the end of the permeability test, the Ti discs were removed from the three-dimensional OMM and an Alamar Blue assay was used for the measurement of residual cells attached to the Ti discs. The penetration of the HTO through the BS for the four types of Ti surfaces was not significantly different, and there was no significant difference in the viability of residual cells that attached to the Ti surfaces. The BS of the tissue-engineered oral mucosa around the four types of Ti surface topographies was not significantly different.

scholarly journals Effect of Hyaluronan on Developmental Competence and Quality of Oocytes and Obtained Blastocysts fromIn VitroMaturation of Bovine Oocytes

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Jolanta Opiela ◽  
Joanna Romanek ◽  
Daniel Lipiński ◽  
Zdzisław Smorąg
Keyword(s):  
Dna Fragmentation ◽  
Oocyte Maturation ◽  
Developmental Competence ◽  
Meiotic Maturation ◽  
Nuclear Maturation ◽  
Significant Difference ◽  
The Mean ◽  
Bovine Oocytes

The objective of the present study was to evaluate the effect of hyaluronan (HA) during IVM on meiotic maturation, embryonic development, and the quality of oocytes, granulosa cells (GC), and obtained blastocysts. COCs were maturedin vitroin control medium and medium with additional 0.035% or 0.07% of exogenous HA. The meiotic maturity did not differ between the analysed groups. The best rate and the highest quality of obtained blastocysts were observed when 0.07% HA was used. A highly significant difference (P<0.001) was noted in the mean number of apoptotic nuclei per blastocyst and in the DCI between the 0.07% HA and the control blastocysts (P<0.01). Our results suggest that addition of 0.035% HA and 0.07% HA to oocyte maturation media does not affect oocyte nuclear maturation and DNA fragmentation. However, the addition of 0.07% HA during IVM decreases the level of blastocysts DNA fragmentation. Finally, our results suggest that it may be risky to increase the HA concentration during IVM above 0.07% as we found significantly higherBaxmRNA expression levels in GC cultured with 0.07% HA. The final concentration of HA being supplemented to oocyte maturation media is critical for the success of the IVP procedure.

Screening black gram genotypes under artificially inoculated conditions for powdery mildew resistance

2017 ◽  
Author(s):  
S. Priyanka ◽  
S. Rangaiah ◽  
R. Pavan
Keyword(s):  
Powdery Mildew ◽  
Genetic Resistance ◽  
Resistance Breeding ◽  
Cost Effective ◽  
Black Gram ◽  
Resistant Varieties ◽  
House Condition ◽  
Cent Disease

An effective and durable genetic resistance in black gram genotypes against powdery mildew provides a cost effective and reliable strategy to reduce the yield losses and save quality of the harvest. The identification of potential resistant source(s) is the most crucial step in disease resistance breeding. In the present study, out of 116 genotypes screened, genotype LBG-645 recorded lowest per cent disease severity of 0.77 and was found to be highly resistant to powdery mildew under green house condition. Under in vitro condition also, LBG-645 which was found to be highly resistant with 3.33×103 conidia colony, 0.94 per cent of leaf area covered by powdery mildew and 1.00 colony per leaflet was recorded. Hence, resistant genotype LBG-645 must be used in further breeding programme for the development of resistant varieties of black gram against powdery mildew.

scholarly journals An Improved In Vitro Pyrogen Test To Detect The Presence of Endotoxin Containing Bacteria Using Limulus Amoebocyte Lysate Assay From Pharmaceutical Raw Product

1970 ◽  
Vol 1 (1) ◽  
pp. 76-79
Author(s):  
Tanvir Bashar ◽  
Khondokar Mahzebin Shurovi ◽  
Sanjida Dilshad
Keyword(s):  
Pathogenic Bacteria ◽  
Raw Materials ◽  
Cost Effective ◽  
Drug Preparation ◽  
Effective Manner ◽  
Key Word ◽  
Pseudomonas Species ◽  
Lal Assay

Most of the environmental organism possess endotoxin which presence indicates the organisms are much lethal and for the purposes bacteriological quality of pharmaceutical raw products is much important. In these current study, 2 raw materials out of 10 were found to containing pathogenic bacteria Escherichia coli and Pseudomonas species in the culture medium, that indicates the raw materials were contaminated with the deadly organism. These two raw materials checked for the presence of Endotoxin and both provided positive gel clot by Limulas amoebocyte lysate (LAL) assay. Quality maintenance and assurance is the essential need of Drug preparation in pharmaceutical sector. The result ensure that Pharmaceutical industry should need to follow GMP and HACCP to minimize the contamination for improving the biological safety of the product in a cost-effective manner. Key Word: Limulas amoebocyte lysate (LAL) assay, Gram negative bacteria, endotoxin. doi:10.3329/sjps.v1i1.1812    S. J. Pharm. Sci. 1(1&2): 76-79

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