Characterization of a Cancer Stem Cell-Like Side Population Derived from Human Pancreatic Adenocarcinoma Cells

2010 ◽  
Vol 96 (6) ◽  
pp. 985-992 ◽  
Author(s):  
Shi-Neng Zhang ◽  
Feng-Ting Huang ◽  
Yi-Jun Huang ◽  
Wa Zhong ◽  
Zhong Yu
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Pancreatic Adenocarcinoma ◽  
Side Population ◽  
Human Pancreatic Adenocarcinoma ◽  
Adenocarcinoma Cells

Characterization of stem cell–like cancer cells in immune-competent mice

Blood ◽  
2006 ◽  
Vol 108 (12) ◽  
pp. 3906-3912 ◽  
Author(s):  
Jorg A. Kruger ◽  
Charles D. Kaplan ◽  
Yunping Luo ◽  
He Zhou ◽  
Dorothy Markowitz ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cancer Cells ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Side Population ◽  
Carcinoma Cell Lines ◽  
Tumorigenic Potential ◽  
4T1 Cells

AbstractRecently, the cancer stem cell hypothesis has gained significant recognition as the descriptor of tumorigenesis. Although previous studies relied on transplanting human or rat tumor cells into immunecompromised mice, our study used the Hoechst 33342 dye–based side population (SP) technique to isolate and transplant stem cell–like cancer cells (SCLCCs) from the 4T1 and NXS2 murine carcinoma cell lines into the immune-competent microenvironment of syngeneic mice. 4T1 cells displayed an SP of 2% with a Sca-1highc-Kit–CD45– phenotype, whereas NXS2 cells contained an SP of 0.2% with a Sca-1highCD24highc-Kit–CD45–GD high2 phenotype. Reverse transcription–polymerase chain reaction (RT-PCR) further revealed up-regulation in SP cells of ABCG2, Sca-1, Wnt-1, and TGF-β2. Additionally, 4T1 and NXS2 SP cells exhibited increased resistance to chemotherapy, and 4T1 SP cells also showed an increased ability to efflux doxorubicin, which correlated with a selective increase in the percentage of SP cells found in the tumors of doxorubicin-treated mice. Most importantly, SP cells showed a markedly higher repopulation and tumorigenic potential in vivo, which correlated with an increased number of cells in the SP compartment of SP-derived tumors. Taken together, these results show that we successfully characterized SCLCCs from 2 murine carcinoma cell lines in the immune-competent microenvironment of syngeneic mice.

Garcinol sensitizes human pancreatic adenocarcinoma cells to gemcitabine in association with microRNA signatures

2013 ◽  
Vol 57 (2) ◽  
pp. 235-248 ◽  
Author(s):  
Mansi A. Parasramka ◽  
Shadan Ali ◽  
Sanjeev Banerjee ◽  
Tara Deryavoush ◽  
Fazlul H Sarkar ◽  
...  
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Human Pancreatic Adenocarcinoma ◽  
Adenocarcinoma Cells

Isolation and characterization of breast cancer stem cell‐like phenotype by Oct4 promoter‐mediated activity

2020 ◽  
Vol 235 (11) ◽  
pp. 7840-7848 ◽  
Author(s):  
Zahra Ghanei ◽  
Abbas Jamshidizad ◽  
Morteza Daliri Joupari ◽  
Mehdi Shamsara
Keyword(s):  
Breast Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Breast Cancer Stem Cell ◽  
Isolation And Characterization

scholarly journals 5′-Nucleotidases of chicken gizzard and human pancreatic adenocarcinoma cells are anchored to the plasma membrane via a phosphatidylinositol–glycan

1989 ◽  
Vol 262 (1) ◽  
pp. 33-40 ◽  
Author(s):  
U Stochaj ◽  
K Flocke ◽  
W Mathes ◽  
H G Mannherz
Keyword(s):  
Plasma Membrane ◽  
Pancreatic Adenocarcinoma ◽  
Plasma Membranes ◽  
Chicken Gizzard ◽  
Human Pancreatic Adenocarcinoma ◽  
Adenocarcinoma Cells ◽  
Increased Sensitivity ◽  
Membrane Bound ◽  
Membrane Anchoring ◽  
Pancreatic Adenocarcinoma Cell Line

We have analysed the membrane anchorage of plasma-membrane 5′-nucleotidase, an ectoenzyme which can mediate binding to components of the extracellular matrix. We demonstrated that the purified enzyme obtained from chicken gizzard and a human pancreatic adenocarcinoma cell line were both completely transformed into a hydrophilic form by treatment with phospholipases C and D, cleaving glycosylphosphatidylinositol (GPI). These data indicate the presence of a glycolipid linker employed for membrane anchoring of the 5′-nucleotidase obtained from both sources. Incubation of plasma membranes under identical conditions revealed that about half of the AMPase activity was resistant to GPI-hydrolysing phospholipases. Investigation of the enzymic properties of purified chicken gizzard 5′-nucleotidase revealed only minor changes after removal of the phosphatidylinositol linker. However, cleavage of the membrane anchor resulted in an increased sensitivity towards inhibition by concanavalin A. After tissue fractionation, chicken gizzard 5′-nucleotidase could be obtained as either a membrane-bound or a soluble protein; the latter is suspected to be released from the plasma membrane by endogenous phospholipases. Higher-molecular-mass proteins immuno-cross-reactive with the purified chicken gizzard 5′-nucleotidase were detected as both soluble and membrane-bound forms.

Abstract 3337: TGF-β from niche fibroblasts increases the stemness cancer stem cell-like side population cells in gastric cancer

2012 ◽  
Author(s):  
Tsuyoshi Hasegawa ◽  
Masakazu Yashiro ◽  
Tamami Morisaki ◽  
Naoki Aomatsu ◽  
Toshiki Hirakawa ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Stem Cell ◽  
Cancer Stem Cell ◽  
Side Population ◽  
Side Population Cells

scholarly journals ZIC2 promotes cancer stem cell traits via up-regulating OCT4 expression in lung adenocarcinoma cells

2020 ◽  
Vol 11 (20) ◽  
pp. 6070-6080
Author(s):  
Wang Wei-Hua ◽  
Zhou Ning ◽  
Chen Qian ◽  
Jiang Dao-Wen
Keyword(s):  
Stem Cell ◽  
Cancer Stem Cell ◽  
Lung Adenocarcinoma ◽  
Oct4 Expression ◽  
Adenocarcinoma Cells ◽  
Lung Adenocarcinoma Cells

scholarly journals Anticancer properties of erucin, an H2S-releasing isothiocyanate, on human pancreatic adenocarcinoma cells (AsPC-1)

2019 ◽  
Vol 33 (3) ◽  
pp. 845-855 ◽  
Author(s):  
Valentina Citi ◽  
Eugenia Piragine ◽  
Eleonora Pagnotta ◽  
Luisa Ugolini ◽  
Lorenzo Di Cesare Mannelli ◽  
...  
Keyword(s):  
Pancreatic Adenocarcinoma ◽  
Anticancer Properties ◽  
Human Pancreatic Adenocarcinoma ◽  
Adenocarcinoma Cells
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