scholarly journals A protein-specific monoclonal antibody to rat liver beta 1-->4 galactosyltransferase and its application to immunohistochemistry.

1994 ◽  
Vol 42 (3) ◽  
pp. 363-369 ◽  
Author(s):  
J Kawano ◽  
S Ide ◽  
T Oinuma ◽  
T Suganuma
Keyword(s):  
Monoclonal Antibody ◽  
Rat Liver ◽  
Immunohistochemical Study ◽  
Acinar Cells ◽  
Specific Monoclonal Antibody ◽  
Intense Staining ◽  
Golgi Membranes ◽  
In Trans ◽  
New Protein ◽  
Epididymal Duct

We have produced a new protein-specific monoclonal antibody (MAb) to rat liver beta 1-->4 galactosyltransferase. This MAb, GTL2, was selected as the most reactive IgG to a periodate-treated antigen. Antigen and protein specificities of GTL2 were verified by immunoblotting of a non-glycosylated recombinant protein of human galactosyltransferase and enzymatically deglycosylated rat galactosyltransferase. Using GTL2, an immunohistochemical study was done in rat liver, epididymis, and salivary glands. Intense staining was observed in Golgi areas of epididymal duct epithelial cells, and submandibular and sublingual acinar cells. Hepatocytes showed weaker staining. Immunoelectron microscopic observation revealed that the staining was exclusively localized in trans-Golgi membranes of these cells.

scholarly journals A comparative study of polyclonal and monoclonal antibodies for immunocytochemical localization of cytosolic aspartate aminotransferase in rat liver.

1983 ◽  
Vol 31 (7) ◽  
pp. 920-926 ◽  
Author(s):  
C T Lin ◽  
L H Chen ◽  
T S Chan
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Rat Liver ◽  
Aspartate Aminotransferase ◽  
Polyclonal Antibodies ◽  
Rabbit Antiserum ◽  
Igg Antibody ◽  
Experimental Conditions ◽  
Intense Staining ◽  
Rabbit Igg

The rabbit antiserum and mouse monoclonal hybridoma antibody against porcine cytosolic aspartate aminotransferase (c-AAT) (or cytosolic glutamic oxaloacetic transaminase (c-GOT)) were produced and compared for the localization of c-AAT in rat liver. An indirect immunocytochemical technique was performed using peroxidase-conjugated goat immunoglobulin (Ig) G anti-rabbit IgG and peroxidase-conjugated rabbit IgG anti-mouse IgG as the second antibody. Rats were perfused with paraformaldehyde-lysine-periodate fixative and the liver fragments were immersed in 4% paraformaldehyde and transferred to 10% dimethyl sulfoxide overnight and subjected to cryostat sectioning. The rabbit IgG antibody, 3 individual monoclonal antibodies, and a mixture of these 3 monoclonal antibodies were applied to the tissue sections, respectively, using the same concentration. Under the same experimental conditions, the c-AAT was localized in each individual hepatocyte by both monoclonal and polyclonal antibodies. However, a mixture of three monoclonal antibodies gave stronger staining than a single monoclonal antibody; although two antibodies yield more intense staining than just one, it was still less intense than for three. The conventional rabbit polyclonal antibody against c-AAT produced more reaction product than the combined three monoclonal antibodies. It is concluded that for immunocytochemical study, the use of a single monoclonal antibody is sensitive enough to localize its tissue antigen under the present experimental condition. To obtain a stronger reaction product, a combination of several monoclonal antibodies, at least three or more, may give better staining.

The ultrastructure of acinar cells in the external orbital gland of young and old male rats

1978 ◽  
Vol 36 (2) ◽  
pp. 276-277
Author(s):  
R. Carriere
Keyword(s):  
Young Adulthood ◽  
Acinar Cells ◽  
Cell Number ◽  
Male Rats ◽  
Polyploid Cell ◽  
Secretory Cells ◽  
Age Changes ◽  
Albino Rat ◽  
Golgi Membranes ◽  
Diploid Cells

The external orbital gland of the albino rat exhibits both sexual dimorphism and histological age changes. In males, many cells attain a remarkable degree of polyploidy and an increase of polyploid cell number constitutes the major age change until young adulthood. The acini of young adults have a small lumen and are composed of tall serous cells. Subsequently, many acini acquire a larger lumen with an irregular outline while numerous vacuoles accumulate throughout the secretory cells. At the same time, vesicular acini with a large lumen surrounded by pale-staining low cuboidal diploid cells begin to appear and their number increases throughout old age. The fine structure of external orbital glands from both sexes has been explored and in considering acinar cells from males, emphasis was given to the form of the Golgi membranes and to nuclear infoldings of cytoplasmic constituents.

Therapeutic Potential of SARS-CoV-2-Specific Monoclonal Antibody CR3022

2020 ◽  
Author(s):  
Caroline Atyeo ◽  
Matthew D. Slein ◽  
Stephanie Fischinger ◽  
John Burke ◽  
Alexandra Schӓfer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Therapeutic Potential ◽  
Specific Monoclonal Antibody

SIRPα-specific monoclonal antibody enables antibody-dependent phagocytosis of neuroblastoma cells

2021 ◽  
Author(s):  
Meriem Bahri ◽  
Sareetha Kailayangiri ◽  
Sarah Vermeulen ◽  
Natacha Galopin ◽  
Claudia Rossig ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Neuroblastoma Cells ◽  
Specific Monoclonal Antibody

scholarly journals A new reliable and highly specific monoclonal antibody to detect the C‐terminal region of Silencer of Cytokine Signaling 1 (SOCS1)

2021 ◽  
Author(s):  
Stephanie E. Weissinger ◽  
Malena Zahn ◽  
Ralf Marienfeld ◽  
Claudia Tessmer ◽  
Gerhard Moldenhauer ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Terminal Region ◽  
Cytokine Signaling ◽  
Specific Monoclonal Antibody
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