scholarly journals Conventional and confocal fluorescence microscopy of collagen fibers in the heart.

1993 ◽  
Vol 41 (3) ◽  
pp. 465-469 ◽  
Author(s):  
P C Dolber ◽  
M S Spach
Keyword(s):  
Fluorescence Microscopy ◽  
Collagen Fiber ◽  
Three Dimensional ◽  
Phosphomolybdic Acid ◽  
Collagen Fibers ◽  
Fluorescence Method ◽  
Mechanical Modeling ◽  
Confocal Fluorescence ◽  
Picrosirius Red ◽  
Confocal Microscopic Study

The arrangement of collagen fibers has previously been studied with picrosirius red (PSR) staining and brightfield microscopy. We discovered that PSR staining can also be visualized by fluorescence microscopy. PSR-stained collagen was strongly fluorescent using excitation and barrier filters for rhodamine, and distracting background cytoplasmic fluorescence was drastically reduced with phosphomolybdic acid (PMA) treatment before PSR staining. The PMA-PSR fluorescence method was more sensitive than the brightfield PSR or PMA-PSR method, and permitted confocal microscopic study. We applied the method to the study of collagen fiber three-dimensional arrangement in perimysial and endomysial septa of the heart, showing the three-dimensional course of the fibers in stereo views generated by confocal microscopy. The PMA-PSR fluorescence method should be generally useful for accurately determining collagen fiber three-dimensional arrangement, a necessary prelude to mechanical modeling of collagen-reinforced tissues.

Confocal fluorescence microscopy with the tandem scanning light microscope

1989 ◽  
Vol 94 (4) ◽  
pp. 617-624
Author(s):  
S.J. Wright ◽  
J.S. Walker ◽  
H. Schatten ◽  
C. Simerly ◽  
J.J. McCarthy ◽  
...  
Keyword(s):  
High Resolution ◽  
Fluorescence Microscopy ◽  
Light Microscope ◽  
Three Dimensional ◽  
Three Dimensions ◽  
Complex Structures ◽  
Charge Coupled Device ◽  
Confocal Fluorescence ◽  
Cooled Ccd ◽  
Cellular Components

Applications of the tandem scanning confocal microscope (TSM) to fluorescence microscopy and its ability to resolve fluorescent biological structures are described. The TSM, in conjunction with a cooled charge-coupled device (cooled CCD) and conventional epifluorescence light source and filter sets, provided high-resolution, confocal data, so that different fluorescent cellular components were distinguished in three dimensions within the same cell. One of the unique features of the TSM is the ability to image fluorochromes excited by ultraviolet light (e.g. Hoechst, DAPI) in addition to fluorescein and rhodamine. Since the illumination is dim, photobleaching is insignificant and prolonged viewing of living specimens is possible. Series of optical sections taken in the Z-axis with the TSM were reproduced as stereo images and three-dimensional reconstructions. These data show that the TSM is potentially a powerful tool in fluorescence microscopy for determining three-dimensional relationships of complex structures within cells labeled with multiple fluorochromes.

Developing Antler as a Model System of Fibrillogenesis

1982 ◽  
Vol 40 ◽  
pp. 298-299
Author(s):  
D.P. Speer ◽  
C.W. Kischer
Keyword(s):  
Collagen Fiber ◽  
Bone Structure ◽  
Three Dimensional ◽  
Polarized Light ◽  
Collagen Fibers ◽  
Cartilage Growth ◽  
Functional Roles ◽  
Growth Cartilage ◽  
Mammalian Tissues ◽  
Microscopy Techniques

Collagen fibers provide tensile strength to mammalian tissues and in many instances play morphogenetic as well as structural functional roles. While the directional orientation of these collagen fibers determines the tensile vectors within tissues, the three dimensional architecture of these collagen fiber systems has not been widely studied. Recent studies of the collagen architecture of articular cartilage, growth cartilage, and the developing deer's antler have employed physical optical principles with polarized light microscopy to demonstrate fiber fine structure in large areas of tissue. These studies have been supported by and complement those using electron microscopy techniques. The present study shows the fibrous architecture of the antler velvet, a tissue responsible for the annual production of a massive true bone structure in the deer.

Unsupervised noise removal algorithms for three-dimensional confocal fluorescence microscopy

1992 ◽  
Vol 23 (4) ◽  
pp. 447-461 ◽  
Author(s):  
Badrinath Roysam ◽  
Anoop K. Bhattacharjya ◽  
Chukka Srinivas ◽  
Donald H. Szarowski ◽  
James N. Turner
Keyword(s):  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Noise Removal ◽  
Confocal Fluorescence Microscopy ◽  
Confocal Fluorescence

Flow of Microsphere Solutions in Converging Microchannels

2008 ◽  
Author(s):  
Alexis B. Reedy ◽  
Nelson Macken
Keyword(s):  
Reynolds Number ◽  
Fluorescence Microscopy ◽  
Three Dimensional ◽  
Main Channel ◽  
Two Dimensional ◽  
Mixture Flow ◽  
Water Flows ◽  
Combined Flow ◽  
Flow Reynolds Number ◽  
Confocal Fluorescence

The interface between intersecting microfluidic multicomponent flow is investigated experimentally. The system studied has a main channel and an intersecting (at 90 degrees) daughter channel and the fluid is a water-microsphere mixture. Flow visualization is achieved using confocal fluorescence microscopy. Microsphere solutions of 2% and 4% spheres (by weight) are investigated and compared to each other as well as all water flows. A three-dimensional rendering of the location and shape of the interface at the junction for different flow ratios (daughter flow to total) is examined for a combined flow Reynolds number of approximately one. The interface downstream of the junction is reasonably planer (two-dimensional) and the presence of microspheres does not appear to strongly influence the location of the flow interface.

Flow of Microfluids in Converging Microchannels

2009 ◽  
Author(s):  
Nelson Macken ◽  
Jong Hsien Lim
Keyword(s):  
Reynolds Number ◽  
Fluorescence Microscopy ◽  
Cross Section ◽  
Cross Sections ◽  
Three Dimensional ◽  
Main Channel ◽  
Two Dimensional ◽  
Water Flows ◽  
The Third ◽  
Confocal Fluorescence

The interface between intersecting microfluidic multicomponent flow is investigated experimentally. Three microchannel configurations are studied. Each configuration has a main channel and an intersecting daughter channel. In two configurations, the channel cross sections are equal and square with the intersection either at 90 or 45 degrees. In the third configuration, the intersection is at 90 degrees, the cross sections are square and the daughter cross section is smaller than the main cross section. In the configurations with equal channel cross sections, microsphere solutions of 2, 4 and 7% spheres (by weight) are compared to each other as well as all water flows. Flow visualization is achieved using confocal fluorescence microscopy. A three-dimensional rendering of the location and shape of the interface is examined for a Reynolds number of approximately one. The presence of microspheres does not appear to strongly influence the location of the flow interface. For flows with equal cross section, the interface downstream of the junction is reasonably planer (two dimensional). Strong three-dimensional effects are shown for flows with unequal cross section.

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