scholarly journals Combined immunocytochemistry and enzyme cytochemistry on ultra-thin cryosections: a new method.

1993 ◽  
Vol 41 (11) ◽  
pp. 1635-1639 ◽  
Author(s):  
T Takizawa ◽  
J M Robinson
Keyword(s):  
Adverse Effects ◽  
Colloidal Gold ◽  
Antibody Binding ◽  
New Method ◽  
Immunocytochemical Localization ◽  
Binding Properties ◽  
Enzyme Cytochemistry

We combined immunocytochemistry and enzyme cytochemistry to localize two different proteins on the same ultrathin cryosection. In this method the immunocytochemical localization is visualized with colloidal gold probes and the enzyme cytochemical detection is achieved with cerium as the capture agent. The immunocytochemistry is conducted first so that any potential adverse effects of the enzyme cytochemical procedure will not alter the antibody binding properties of the cryosections.

scholarly journals A New Method to Enhance Contrast of Ultrathin Cryosections for Immunoelectron Microscopy

2003 ◽  
Vol 51 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Toshihiro Takizawa ◽  
Clark L. Anderson ◽  
John M. Robinson
Keyword(s):  
Immunoelectron Microscopy ◽  
Staining Method ◽  
Microscopic Level ◽  
New Method ◽  
Electron Microscopic Level ◽  
Positive Staining ◽  
Immunocytochemical Localization ◽  
Electron Microscopic ◽  
Morphological Detail ◽  
Ultrathin Cryosections

Adequate contrast of ultrathin cryosections is crucial for evaluating morphological detail to assess immunocytochemical localization at the electron microscopic level. We have developed a positive staining method for achieving contrast in ultrathin cryosections, from tissue fixed only in paraformaldehyde, that provides excellent contrast at the electron microscopic level.

Feature Edge Extraction Via Angle-Based Edge Collapsing and Recovery

2018 ◽  
Vol 18 (2) ◽  
Author(s):  
Soji Yamakawa ◽  
Kenji Shimada
Keyword(s):  
Adverse Effects ◽  
Computer Aided Design ◽  
Region Growing ◽  
New Method ◽  
Small Radius ◽  
Edge Extraction ◽  
Line Segments ◽  
Computer Aided ◽  
Downstream Processes ◽  
Aided Design

This paper presents a new method for extracting feature edges from computer-aided design (CAD)-generated triangulations. The major advantage of this method is that it tends to extract feature edges along the centroids of the fillets rather than along the edges where fillets are connected to nonfillet surfaces. Typical industrial models include very small-radius fillets between relatively large surfaces. While some of those fillets are necessary for certain types of analyses, many of them are irrelevant for many other types of applications. Narrow fillets are unnecessary details for those applications and cause numerous problems in the downstream processes. One solution to the small-radius fillet problem is to divide the fillets along the centroid and then merge each fragment of the fillet with nonfillet surfaces. The proposed method can find such fillet centroids and can substantially reduce the adverse effects of such small-radius fillets. The method takes a triangulated geometry as input and first simplifies the model so that small-radius, or “small,” fillets are collapsed into line segments. The simplification is based on the normal errors and therefore is scale-independent. It is particularly effective for a shape that is a mix of small and large features. Then, the method creates segmentation in the simplified geometry, which is then transformed back to the original shape while maintaining the segmentation information. The groups of triangles are expanded by applying a region-growing technique to cover all triangles. The feature edges are finally extracted along the boundaries between the groups of triangles.

scholarly journals A new method for transfer of polyethylene glycol-embedded tissue sections to silanated slides for immunocytochemistry.

1991 ◽  
Vol 39 (4) ◽  
pp. 537-540 ◽  
Author(s):  
K X Gao ◽  
J D Godkin
Keyword(s):  
Polyethylene Glycol ◽  
Colloidal Gold ◽  
Cell Structure ◽  
High Sensitivity ◽  
Antigen Detection ◽  
New Method ◽  
Retinol Binding Protein ◽  
Tissue Sections ◽  
Glass Slides ◽  
Structural Preservation

Polyethylene glycol (PEG) is an excellent embedding medium for immunohistochemical studies. It provides structural preservation superior to frozen sections and increased sensitivity of antigen detection compared with paraffin sections. One limitation of PEG embedment is that PEG sections are difficult to handle and adhere poorly to glass slides. Here we present a simple and effective method for embedding tissues in PEG and transferring the resultant sections onto silanated glass slides. In addition, a method for silver enhanced colloidal gold immunostaining was combined with common dye staining to demonstrate the excellent structure preservation and sensitive antigen detection. Bovine chorionic membrane was fixed with Bouin's fixative, embedded in polyethylene glycol (PEG) 1500, cut into 5-microns sections, flattened over agarose blocks (10 x 10 x 2 mm3), and blotted onto Digene silanated slides. Slides were then washed in PBS, which removed the PEG and agarose blocks. Tissue sections were immunocytochemically stained with dilute antiserum raised in a rabbit against purified bovine placental retinol binding protein (bpRBP). Sections were washed and incubated with 1-nm colloidal gold-labeled goat anti-rabbit IgG. The immunogold particles were enhanced by silver staining (IGSS). Specimens were observed and photographed with an Olympus epipolarization microscope. The new method offered excellent morphological preservation of cell structure and the epipolarization microscopy provided high sensitivity for detection of specific immunogold-silver particles.

ChemInform Abstract: Synthesis and Antibody Binding Properties of Glycodendrimers Bearing the Tumor Related T-Antigen.

ChemInform ◽  
2001 ◽  
Vol 32 (19) ◽  
pp. no-no
Author(s):  
Myung-Gi Baek ◽  
Kate Rittenhouse-Olson ◽  
Rene Roy
Keyword(s):  
T Antigen ◽  
Antibody Binding ◽  
Binding Properties

Immunocytochemical Localization of Relaxin in Human Decidua and Fetal Membrane by Colloidal Gold Labeled Antibody

1987 ◽  
Vol 10 (2) ◽  
pp. 99-100
Author(s):  
Guian Chen ◽  
Jules M. Elias ◽  
Bernard G. Steinetz ◽  
Linda Tseng
Keyword(s):  
Colloidal Gold ◽  
Fetal Membrane ◽  
Immunocytochemical Localization ◽  
Human Decidua

A new cytochemical method: Combined enzyme cytochemistry and immunocytochemistry on ultrathin cryosections

1993 ◽  
Vol 51 ◽  
pp. 326-327
Author(s):  
Toshihiro Takizawa ◽  
John M. Robinson
Keyword(s):  
Cell Types ◽  
Uranyl Acetate ◽  
New Method ◽  
Human Neutrophils ◽  
Enzyme Cytochemistry ◽  
Cytochemical Method ◽  
Antigenic Sites ◽  
Cacodylate Buffer ◽  
Ultrathin Cryosections ◽  
In Cells

Cryo-ultramicrotomy has been a useful method for immunocytochemistry because it can preserve the ultrastructure of cells and also display antigenic sites in cells. Recently, cryo-ultramicrotomy has been successfully applied to enzyme cytochemistry for the demonstration of some phosphatases with lead as the capture metal. However, this application has some problems. The lead reaction product is unstable with uranyl acetate staining, thus leading to poor ultrastructural detail. The purpose of this study is to develop the new method of enzyme cytochemistry using cerium as the capture metal on ultrathin cryosections labeled with immunogold probes. This method should be of importance for demonstrating the ultrastructure localization of certain enzyme and the simultaneous localization of antigens in a variety of cell types.Freshly isolated human neutrophils were fixed with paraformaldehyde and/or glutaraldehyd in 0.1 M cacodylate buffer (pH 7.2) with 5 % sucrose, then pelleted into 10 % gelatin and subsequently infiltrated with 2.3 M sucrose.

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