scholarly journals Distribution of myoepithelial cells and basement membrane proteins in the resting, pregnant, lactating, and involuting rat mammary gland.

1982 ◽  
Vol 30 (7) ◽  
pp. 667-676 ◽  
Author(s):  
M J Warburton ◽  
D Mitchell ◽  
E J Ormerod ◽  
P Rudland
Keyword(s):  
Mammary Gland ◽  
Membrane Proteins ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Basement Membrane ◽  
Myoepithelial Cells ◽  
Type I ◽  
Continuous Layer ◽  
Basement Membrane Proteins ◽  
Rat Mammary Gland

Using antisera to specific proteins, the localization of the rat mammary parenchymal cells (both epithelial and myoepithelial), the basement membrane, and connective tissue components has been studied during the four physiological stages of the adult rat mammary gland, viz. resting, pregnant, lactating, and involuting glands. Antisera to myosin and prekeratin were used to localize myoepithelial cells, antisera to rat milk fat globule membrane for epithelial cells, antisera to laminin and type IV collagen to delineate the basement membrane and antisera to type I collagen and fibronectin as markers for connective tissue. In the resting, virgin mammary gland, myoepithelial cells appear to form a continuous layer around the epithelial cells and are in turn surrounded by a continuous basement membrane. Antiserum to fibronectin does not delineate the basement membrane in the resting gland. The ductal system is surrounded by connective tissue. Only the basal or myoepithelial cells in the terminal end buds of neonatal animals demonstrate cytoplasmic staining for basement membrane proteins, indicating active synthesis of these proteins during this period. In the secretory alveoli of the lactating rat, the myoepithelial cells no longer appear to form a continuous layer beneath the epithelial cells and in many areas the epithelial cells appear to be in contact with the basement membrane. The basement membrane in the lactating gland is still continuous around the ducts and alveoli. In the lactating gland, fibronectin appears to be located in the basement membrane region in addition to being a component of the stroma. During involution, the alveoli collapse, and appear to be in a state of dissolution. The basement membrane is thicker and is occasionally incomplete, as also are the basket-like myoepithelial structures. Basement membrane components can also be demonstrated throughout the collapsed alveoli.

Characterization of rat mammary cell types in primary culture: lectins and antisera to basement membrane and intermediate filament proteins as indicators of cellular heterogeneity

1985 ◽  
Vol 79 (1) ◽  
pp. 287-304
Author(s):  
M.J. Warburton ◽  
S.A. Ferns ◽  
C.M. Hughes ◽  
P.S. Rudland
Keyword(s):  
Membrane Proteins ◽  
Epithelial Cells ◽  
Basement Membrane ◽  
Intermediate Filament ◽  
Cell Types ◽  
Myoepithelial Cells ◽  
Epithelioid Cells ◽  
Intermediate Filament Proteins ◽  
Basement Membrane Proteins

Three morphologically distinct major cell types were observed in primary cultures obtained from the mammary parenchyma of glands from virgin rats. These cell types consisted of small cuboidal epithelial cells, larger epithelioid cells and elongated cells. We have investigated the distribution of the basement membrane proteins laminin and type IV collagen, and the intermediate filament proteins vimentin and prekeratin, in these three cell types using immunofluorescence techniques. Antisera to the basement membrane proteins stain the large epithelioid cells and the elongated cells, but do not stain the small cuboidal cells. Polyclonal antiserum to keratin stains all the small cuboidal and large epithelioid cells, but only a small subpopulation of the elongated cells. However, a monoclonal antibody to keratin, LP34, stains only the large cuboidal and a proportion of the elongated cells. Vimentin antiserum fails to stain the small cuboidal cells but stains all the large epithelioid and elongated cells. In addition, peanut lectin, which binds only to ductal lining epithelial cells in the virgin rat mammary gland in vivo after their treatment with neuraminidase, binds to the small cuboidal cells after neuraminidase treatment but not to the other cell types. However, Griffonia simplicifolia agglutinin I, which specifically stains myoepithelial cells in vivo, binds to the large epithelioid and elongated cells but not to the small cuboidal cells. These results suggest that the small cuboidal cells are related to mammary ductal epithelial cells whereas the large epithelial and elongated cells have some characteristics of myoepithelial cells.

scholarly journals Enhanced synthesis of gelatinase and stromelysin by myoepithelial cells during involution of the rat mammary gland.

1992 ◽  
Vol 40 (5) ◽  
pp. 697-703 ◽  
Author(s):  
S R Dickson ◽  
M J Warburton
Keyword(s):  
Mammary Gland ◽  
Basement Membrane ◽  
Gelatin Zymography ◽  
Mammary Glands ◽  
Myoepithelial Cells ◽  
Immunofluorescence Staining ◽  
Rat Mammary Gland

During the involution of the mammary gland there is destruction of the basement membrane as the secretory alveolar structures degenerate. Immunofluorescence staining of sections of rat mammary gland with antibodies to 72 KD gelatinase (MMP-2) and stromelysin (MMP-3) revealed increased production of these two proteinases during involution. This increased expression was mostly restricted to myoepithelial cells. Increased expression during involution was also demonstrated by immunoblotting techniques. Gelatin zymography indicated that the predominant metalloproteinase present in involuting rat mammary glands was a 66 KD gelatinase.

scholarly journals Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin.

1986 ◽  
Vol 34 (8) ◽  
pp. 1037-1046 ◽  
Author(s):  
A Sonnenberg ◽  
H Daams ◽  
M A Van der Valk ◽  
J Hilkens ◽  
J Hilgers
Keyword(s):  
Monoclonal Antibodies ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Basement Membrane ◽  
Cell Types ◽  
Mouse Mammary Gland ◽  
Type I ◽  
Basal Cells ◽  
Alveolar Cells ◽  
Luminal Type

The development of the mouse mammary gland was studied immunohistochemically using monoclonal antibodies against cell surface and basement membrane proteins and a polyclonal antibody against keratin. We have identified three basic cell types: basal, myoepithelial, and epithelial cells. The epithelial cells can be subdivided into three immunologically related cell types: luminal type I, luminal type II, and alveolar cells. These five cell types appear at different stages of mammary gland development and have either acquired or lost one of the antibody-defined antigens. The cytoplasmic distribution of several of these antigens varied according to the location of the cells within the mammary gland. Epithelial cells which did not line the lumen expressed antigens throughout the cytoplasm. These antigens were demonstrated on the apical site in situations where the cells lined the lumen. One antigen became increasingly basolateral as the cells became attached to the basement membrane. The basal cells synthesize laminin and deposit it at the cell base. They are present in endbuds and ducts and are probably the stem cells of the mammary gland. Transitional forms have been demonstrated which developmentally link these cells with both myoepithelial and (luminal) epithelial cells.

A new monoclonal antibody to a cell-surface antigen that distinguishes luminal epithelial and myoepithelial cells in the rat mammary gland

1989 ◽  
Vol 94 (3) ◽  
pp. 545-552
Author(s):  
R.S. Mahendran ◽  
M.J. O'Hare ◽  
M.G. Ormerod ◽  
P.A. Edwards ◽  
R.A. McIlhinney ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Milk Fat ◽  
Single Cells ◽  
Myoepithelial Cells ◽  
Stages Of Development ◽  
Leukaemia Cell Line ◽  
Flow Cytometric ◽  
Rat Mammary Gland

A monoclonal antibody (25.5) has been produced that recognises luminal epithelial cells of the rat mammary gland. This antibody together with monoclonal anti-CALLA antibodies, which react with mammary myoepithelial cells, has been used in biochemical, immunocytochemical and flow cytometric studies. Antibody 25.5 bound to proteins of molecular weight 70K and 25K (K = 10(3) Mr) in both the rat milk fat globule membrane and in single cell suspensions prepared from the virgin adult rat mammary gland. Anti-CALLA antibody (J5), recognised a 93–100K protein in the gland extracts, which co-electrophoresed with the CALLA/CD-10 antigen from NALM-6 acute lymphoblastic leukaemia cell line. Antibody 25.5 bound to the luminal surface of rat mammary epithelial cells at all stages of development from neonatal through to pregnancy, lactation and involution. CALLA immunoreactive staining has previously been shown on basally located presumptive myoepithelial cells at all stages of development. Flow cytometric analyses demonstrated that 25.5 and anti-CALLA antibodies stained independent cell populations in suspensions of single cells prepared from purified epithelial elements from the mammary gland of adult virgin rat.

Sign in / Sign up

Export Citation Format

Share Document