scholarly journals Discrete Distribution of binding sites for Dolichos biflorus agglutinin (DBA) and for peanut agglutinin (PNA) in mouse organ tissues.

1981 ◽  
Vol 29 (7) ◽  
pp. 779-780 ◽  
Author(s):  
M Watanabe ◽  
T Muramatsu ◽  
H Shirane ◽  
K Ugai
Keyword(s):  
Binding Sites ◽  
Cell Lineage ◽  
Discrete Distribution ◽  
Fluorescein Isothiocyanate ◽  
Superficial Layer ◽  
Pancreatic Ducts ◽  
Peanut Agglutinin ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin ◽  
Collecting Tubules

The distribution of binding sites for two lectins with different specificities was studied in adult mice by staining paraffin sections with lectins labeled either with fluorescein isothiocyanate or horseradish peroxidase. Binding sites for Dolichos biflorus agglutinin, a lectin specific to terminal alpha-N-acetylgalactosamine residue, were detected only in several restricted regions, such as collecting tubules and Bowman's capsules of the kidney, bile ducts, pancreatic ducts, sperms, oocytes, some secreting cells, and the secreted mucin itself. Binding sites for peanut agglutinin (PNA), a lectin specific to terminal beta-galactosyl residue, were distributed more widely. However, differentiation-dependent alterations in the expression of PNA binding sites have been observed in two types of cell lineage. During the course of spermatogenesis, the binding sites were expressed at the stage of spermatocytes. In the epithelium of esophagus, the binding sites were present in cells of the superficial layer.

The crypt cycle in mouse small intestinal epithelium

1994 ◽  
Vol 107 (12) ◽  
pp. 3271-3279 ◽  
Author(s):  
Y.Q. Li ◽  
S.A. Roberts ◽  
U. Paulus ◽  
M. Loeffler ◽  
C.S. Potten
Keyword(s):  
Binding Sites ◽  
Laboratory Mouse ◽  
Subsequent Development ◽  
Fission Process ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin ◽  
Adult Mice ◽  
Crypt Fission ◽  
Small Intestinal ◽  
Peroxidase Conjugate

We have used a mutation-induced marker system in the intestine of mice heterozygous at the Dlb-1 locus, which determines the expression of binding sites for the lectin Dolichos biflorus agglutinin, and the frequency of clustering of mutated crypts with time as a means of investigating the frequency of the crypt fission process and the crypt cycle. Whole-mount preparations from heterozygous Dlb-1b/Dlb-1a mice were stained with a peroxidase conjugate of Dolichos biflorus agglutinin. Mutations at the Dlb-1b locus in crypt stem cells result in loss of DBA-Px binding in these cells and subsequently their progeny, which eventually results in a rare isolated single, unstained crypt. The subsequent development of pairs, triplets and clusters of negative staining crypts has been assumed to be the result of crypt fission. The frequency of these fission events has been measured in control untreated mice. These negative crypts are the result of spontaneous mutations. We have also looked at mutated crypts after treatment with N-nitroso-N-ethylurea or N-methyl-N'-nitro-N-nitrosoguanidine of young adult mice, which elevates the number of mutations. Our results suggest that the crypt cycle in control animals is very long, 187 +/- 44 weeks (3.6 years, i.e. essentially the life of a laboratory mouse). This implies that about a third of the crypts may divide once in the life of a mouse. After sufficient time for conversion of mixed crypts to monophenotypic crypts after mutagen treatment several clusters of negative crypts were seen.(ABSTRACT TRUNCATED AT 250 WORDS)

Analysis of Glycoproteins from Nasal Polyps and Papillomas by Affinity Chromatography

1984 ◽  
Vol 93 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Katsunori Fukuda ◽  
Hirofumi Matsuyama ◽  
Kohzo Fukami ◽  
Masayuki Ozawa ◽  
Takashi Muramatsu ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Affinity Chromatography ◽  
Binding Sites ◽  
Nasal Polyps ◽  
Ricinus Communis ◽  
Sodium Dodecyl ◽  
Peanut Agglutinin ◽  
Con A ◽  
Dolichos Biflorus ◽  
Ricinus Communis Agglutinin

Glycoproteins were isolated from the particulate fraction of four nasal polyps and three nasal papillomas by affinity chromatography on lectins conjugated with agarose (Concanavalin A [Con A], wheat germ agglutinin [WGA], Ricinus communis agglutinin [RCA], peanut agglutinin [PNA], and Dolichos biflorus agglutinin [DBA]). The glycoprotein mixtures so isolated were then analyzed by sodium dodecyl sulfate gel electrophoresis. Glycoprotein profiles of nasal polyps were similar to each other, but were distinctively different from those of nasal papillomas. Binding sites for Con A, WGA, and RCA isolated from nasal papillomas contained intense bands with a molecular weight less than 15,000 daltons, which were absent in nasal polyps. The major component of PNA-binding sites of nasal polyps is of a molecular weight of 65,000 daltons, which was not detected in nasal papillomas.

scholarly journals Distribution of Glycoconjugates in the Kidney Studied by Use of Labeled Lectins

1983 ◽  
Vol 31 (1A_suppl) ◽  
pp. 139-144 ◽  
Author(s):  
F. Murata ◽  
S. Tsuyama ◽  
S. Suzuki ◽  
H. Hamada ◽  
M. Ozawa ◽  
...  
Keyword(s):  
Binding Sites ◽  
Ricinus Communis ◽  
Collecting Duct ◽  
Rat Kidney ◽  
Light And Electron Microscopy ◽  
Peanut Agglutinin ◽  
Dolichos Biflorus ◽  
Ricinus Communis Agglutinin ◽  
Brush Borders ◽  
Convoluted Tubules

Distribution of glycoconjugates in different areas of the rat kidney was studied by light and electron microscopy using six different horseradish peroxidase-labeled lectins. Glomeruli and brush borders of the proximal tubules reacted differently to these lectins, which indicated differences in the carbohydrate compositions of those regions. The ascending limb of Henle's loop (ALH) had strong binding sites for peanut agglutinin (PNA) and soybean agglutinin (SBA). Dolichos biflorus agglutinin (DBA) did not stain the cells of ALH but did stain those of distal convoluted tubules (DCT). DBA is a good marker for distinguishing ALH from DCT. DBA, PNA, and SBA were also good markers of the collecting duct. Ricinus communis agglutinin (RCA-1) and wheat germ agglutinin (WGA) diffusely stained the various components of different parts of the kidney.

scholarly journals The gene controlling the binding sites of Dolichos biflorus agglutinin, Dlb-1, is on chromosome 11 of the mouse

1986 ◽  
Vol 47 (2) ◽  
pp. 125-129 ◽  
Author(s):  
H. G. Uiterdijk ◽  
B. A. J. Ponder ◽  
M. F. W. Festing ◽  
J. Hilgers ◽  
L. Skow ◽  
...  
Keyword(s):  
Intestinal Epithelium ◽  
Vascular Endothelium ◽  
Recombinant Inbred ◽  
Binding Sites ◽  
Inbred Strains ◽  
Recombinant Inbred Strains ◽  
Binding Pattern ◽  
Chromosome 11 ◽  
Dolichos Biflorus ◽  
Dolichos Biflorus Agglutinin

SUMMARYA locus, Dlb-1, controlling the binding of the lectin from Dolichos biflorus to the intestinal epithelium and vascular endothelium of mice has been located on chromosome 11, 3.1 ± 1.4 centimorgans proximal to Rex, using recombinant inbred strains and conventional backcrosses with three-point linkage tests.The two alleles so far discovered at this locus behave unusually. The Dlb-1a allele causes binding to the vascular endothelium but not to the intestinal epithelium while Dlb-1b induces the exact reciprocal binding pattern, and the heterozygote shows both patterns.

scholarly journals GATA2 regulates mast cell identity and responsiveness to antigenic stimulation by promoting chromatin remodeling at super-enhancers

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Yapeng Li ◽  
Junfeng Gao ◽  
Mohammad Kamran ◽  
Laura Harmacek ◽  
Thomas Danhorn ◽  
...  
Keyword(s):  
Mast Cell ◽  
Binding Sites ◽  
Cell Lineage ◽  
Allergic Inflammation ◽  
Chromatin Accessibility ◽  
Antigenic Stimulation ◽  
Parasitic Infections ◽  
Cell Identity ◽  
Super Enhancer ◽  
Determining Factors

AbstractMast cells are critical effectors of allergic inflammation and protection against parasitic infections. We previously demonstrated that transcription factors GATA2 and MITF are the mast cell lineage-determining factors. However, it is unclear whether these lineage-determining factors regulate chromatin accessibility at mast cell enhancer regions. In this study, we demonstrate that GATA2 promotes chromatin accessibility at the super-enhancers of mast cell identity genes and primes both typical and super-enhancers at genes that respond to antigenic stimulation. We find that the number and densities of GATA2- but not MITF-bound sites at the super-enhancers are several folds higher than that at the typical enhancers. Our studies reveal that GATA2 promotes robust gene transcription to maintain mast cell identity and respond to antigenic stimulation by binding to super-enhancer regions with dense GATA2 binding sites available at key mast cell genes.

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