scholarly journals A rapid method for immunofluorescent staining of paraffin sections using iron-containing protein A microspheres.

1981 ◽  
Vol 29 (7) ◽  
pp. 870-873 ◽  
Author(s):  
K J Widder ◽  
A E Senyei ◽  
J L Burchette ◽  
H Ovadia ◽  
P Y Paterson
Keyword(s):  
Rapid Method ◽  
Protein A ◽  
Fluorescein Isothiocyanate ◽  
Immunofluorescent Staining ◽  
Staphylococcal Protein A ◽  
Paraffin Sections ◽  
Formalin Fixed Paraffin ◽  
Blue Reaction ◽  
Antigen Antibody ◽  
Formalin Fixed

A method to rapidly perform immunofluorescence or light microscopic staining on formalin-fixed paraffin sections has been devised utilizing magnetic albumin microspheres containing Staphylococcal protein A. Because the protein A constituent of the microspheres has the property of binding the Fc portion of immunoglobulin G (IgG) class antibodies, the microspheres can be used to rapidly bind antigen-antibody complexes by the Fc portion of the antibody. Deparaffinized sections were stained with fluorescein isothiocyanate-conjugated antibody (IgG fractions) by standard techniques, after which the protein A microspheres were layered over the sections. Distinct fluorescence of sections was noted with the addition of the microspheres, whereas only autofluorescence was present with direct staining alone. The microspheres were also visualized by light microscopy by a subsequent Prussian blue reaction, staining the Fe3O4 within the microsphere matrix. This method represents a more rapid method for identifying antigens in tissues embedded in paraffin than has previously been reported.

scholarly journals Antigen retrieval technique utilizing citrate buffer or urea solution for immunohistochemical demonstration of androgen receptor in formalin-fixed paraffin sections.

1993 ◽  
Vol 41 (11) ◽  
pp. 1599-1604 ◽  
Author(s):  
S R Shi ◽  
B Chaiwun ◽  
L Young ◽  
R J Cote ◽  
C R Taylor
Keyword(s):  
Androgen Receptor ◽  
Enzymatic Digestion ◽  
Antigen Retrieval ◽  
Urea Solution ◽  
Paraffin Sections ◽  
Buffer Solution ◽  
Solution Ph ◽  
Citrate Buffer ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed

We developed a staining protocol for demonstration of androgen receptor (AR) in formalin-fixed, paraffin-embedded tissue sections. The method is based on the antigen retrieval microwave (MW) heating technique. Results are compared with different types of enzyme digestion pre-treatments. The strongest immunostaining signal and clearest background were obtained by MW heating of dewaxed paraffin sections in 5% urea or citrate buffer solution (pH 6); pure distilled water gave less consistent results. Enzymatic digestion with pepsin (0.05% in 2 N HCl) for 30 min at room temperature, or trypsin followed by pronase, or pronase digestion alone, also produced enhanced staining of AR in some cases, but there was more nonspecific background, and specific reactivity was less intense. The antigen retrieval MW method can be used to demonstrate AR epitope in prostate tissue after fixation in formalin for as long as 7 days. AR immunolocalization was also compared in frozen and paraffin sections processed from the same specimen of prostate carcinoma tissue and was found to be qualitatively and quantitatively similar. This study also provided new information concerning the basic principles of the antigen retrieval MW method that may be helpful in further development of this technique.

Astrocytes in Formalin-Fixed Paraffin Sections

1969 ◽  
Vol 51 (3_ts) ◽  
pp. 416-419 ◽  
Author(s):  
Anthony G. H. Folliss ◽  
Martin G. Netsky
Keyword(s):  
Paraffin Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed

Immunohistochemical Detection of Canine Adenovirus in Paraffin Sections of Liver

1986 ◽  
Vol 23 (4) ◽  
pp. 478-484 ◽  
Author(s):  
P. M. Rakich ◽  
K. W. Prasse ◽  
P. D. Lukert ◽  
L. M. Cornelius
Keyword(s):  
Immunohistochemical Detection ◽  
Hepatic Inflammation ◽  
Paraffin Sections ◽  
Inflammatory Lesions ◽  
Long Term Stability ◽  
Formalin Fixed Paraffin ◽  
Wide Range ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

An avidin-biotin immunoperoxidase procedure was optimized for detection of canine adenoviral antigens in formalin-fixed, paraffin-embedded liver. Long-term stability of viral antigen was shown by successful demonstration of virus in liver tissue preserved up to six years from dogs with infectious canine hepatitis. This immunohistochemical stain was applied to sections from livers with a wide range of inflammatory lesions. Examination of sections from 53 dogs yielded five livers with small amounts of adenovirus. An additional virus-positive liver was identified from a dog with no hepatic inflammation. Although a cause and effect relationship remains to be determined, these findings suggest a possible connection between canine adenovirus and spontaneous chronic hepatitis.

Immunocytochemical p53 detection by microwave oven heating of routinely formalin-fixed paraffin sections

1993 ◽  
Vol 171 (2) ◽  
pp. 151-152 ◽  
Author(s):  
Daniela Campani ◽  
Denise Cecchetti ◽  
Generoso Bevilacqua
Keyword(s):  
Microwave Oven ◽  
Paraffin Sections ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed

scholarly journals Detection of Legionnaires disease bacteria by direct immunofluorescent staining

1978 ◽  
Vol 8 (3) ◽  
pp. 329-338
Author(s):  
W B Cherry ◽  
B Pittman ◽  
P P Harris ◽  
G A Hebert ◽  
B M Thomason ◽  
...  
Keyword(s):  
Soil Water ◽  
Lung Tissue ◽  
Fluorescein Isothiocyanate ◽  
Immunofluorescence Staining ◽  
Immunofluorescent Staining ◽  
Direct Immunofluorescence ◽  
Clinical Specimens ◽  
Histological Sections ◽  
Legionnaires Disease ◽  
Formalin Fixed

Antisera and fluorescein isothiocyanate conjugates prepared for five strains of the Legionnaires bacteria were tested in both homologous and heterologous staining reactions with 10 isolates of the organism from patients in seven geographic areas. The strains were related but not identical as judged by the results of direct immunofluorescence staining. The conjugates were successfully used to detect Legionnaires disease bacteria in Formalin-fixed lung scrapings, in histological sections, and in fresh lung tissue obtained at biopsy or autopsy. In addition, the labeled antibodies are valuable for staining suspected cultures of the bacterium and for searching for the source of these organisms in soil, water, and other environmental niches. The reagents are highly specific for detecting the Legionnaires organism in clinical specimens.

Sign in / Sign up

Export Citation Format

Share Document