scholarly journals Laser flow cytometry and cancer chemotherapy: detection of intracellular anthracyclines by flow cytometry.

1979 ◽  
Vol 27 (12) ◽  
pp. 1655-1656 ◽  
Author(s):  
A Krishan ◽  
R Ganapathi
Keyword(s):  
Flow Cytometry ◽  
Laser Beam ◽  
Propidium Iodide ◽  
Indirect Method ◽  
Nuclear Dna ◽  
Flow System ◽  
Direct Method ◽  
Present Report ◽  
Intracellular Detection ◽  
Intracellular Fluorescence

The intracellular distribution of important chemotherapeutic antibiotics belonging to the anthracycline group (e.g. adriamycin) can be detected by laser flow cytometry. The indirect method is based on the interference of these compounds with the binding of propidium iodide to the nuclear DNA. While in the direct method, the intracellular fluorescence of these antibiotics is excited and detected with a laser beam in a flow system. The present report demonstrates the use of these two methods for intracellular detection and quantitation of a number of important anthracyclines.

scholarly journals Evolution of Genome Size in Duckweeds (Lemnaceae)

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Wenqin Wang ◽  
Randall A. Kerstetter ◽  
Todd P. Michael
Keyword(s):  
Flow Cytometry ◽  
Genome Size ◽  
Propidium Iodide ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Continuous Increase ◽  
Spirodela Polyrhiza ◽  
Wolffia Arrhiza ◽  
Basic Reference

To extensively estimate the DNA content and to provide a basic reference for duckweed genome sequence research, the nuclear DNA content for 115 different accessions of 23 duckweed species was measured by flow cytometry (FCM) stained with propidium iodide as DNA stain. The 1C-value of DNA content in duckweed family varied nearly thirteen-fold, ranging from 150 megabases (Mbp) in Spirodela polyrhiza to 1,881 Mbp in Wolffia arrhiza. There is a continuous increase of DNA content in Spirodela, Landoltia, Lemna, Wolffiella, and Wolffia that parallels a morphological reduction in size. There is a significant intraspecific variation in the genus Lemna. However, no such variation was found in other studied species with multiple accessions of genera Spirodela, Landoltia, Wolffiella, and Wolffia.

Biomass active fraction evaluated by a direct method and respirometric techniques

2002 ◽  
Vol 46 (1-2) ◽  
pp. 371-379 ◽  
Author(s):  
G. Andreottola ◽  
P. Foladori ◽  
A. Gelmini ◽  
G. Ziglio
Keyword(s):  
Flow Cytometry ◽  
Indirect Method ◽  
Wastewater Treatment Plants ◽  
Direct Method ◽  
Bacterial Biomass ◽  
Active Fraction ◽  
Active Biomass ◽  
Viable Bacteria ◽  
Viable Biomass ◽  
A Direct Method

The knowledge of the active biomass amount and its characterisation is of primary importance for the management and for the design of wastewater treatment plants on the basis of the recently developed models. OUR curves obtained in aerobic exponential growth tests are proposed by several authors as an indirect method to estimate the active fraction. The aim of this work is the application of a direct method to measure the viable biomass based on flow cytometry techniques and the comparison with the active fraction obtained from respirometric tests. To assess the viable fraction of a biomass expressed in terms of COD concentration it is necessary to estimate the biovolume of viable bacteria and to know the specific carbon content per cell. For the investigated activated sludge samples, the bacterial biomass measured by FCM was 588 mgCOD L−1 on average in a two-months period. This value of active fraction corresponds to 14% of particulate COD. Active biomass values measured with the respirometric approach were consistent but generally higher than those obtained by FCM.

Estimation of nuclear DNA content by flow cytometry in fishes of the genus Xiphophorus

1989 ◽  
Vol 94 (3) ◽  
pp. 465-468 ◽  
Author(s):  
Terrence R. Tiersch ◽  
Robert W. Chandler ◽  
Klaus D. Kallman ◽  
Stephen S. Wachtel
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

First estimates of genome size in ribbon worms (phylum Nemertea) using flow cytometry and Feulgen image analysis densitometry

2014 ◽  
Vol 92 (10) ◽  
pp. 847-851 ◽  
Author(s):  
Kelly L. Mulligan ◽  
Terra C. Hiebert ◽  
Nicholas W. Jeffery ◽  
T. Ryan Gregory
Keyword(s):  
Flow Cytometry ◽  
Image Analysis ◽  
Body Size ◽  
Genome Size ◽  
Positive Relationship ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Size Estimation ◽  
Size Diversity ◽  
Size Estimates

Ribbon worms (phylum Nemertea) are among several animal groups that have been overlooked in past studies of genome-size diversity. Here, we report genome-size estimates for eight species of nemerteans, including representatives of the major lineages in the phylum. Genome sizes in these species ranged more than fivefold, and there was some indication of a positive relationship with body size. Somatic endopolyploidy also appears to be common in these animals. Importantly, this study demonstrates that both of the most common methods of genome-size estimation (flow cytometry and Feulgen image analysis densitometry) can be used to assess genome size in ribbon worms, thereby facilitating additional efforts to investigate patterns of variability in nuclear DNA content in this phylum.

scholarly journals Brief communication. First evaluation of nuclear DNA content in Setaria genus by flow cytometry

1998 ◽  
Vol 89 (6) ◽  
pp. 556-559 ◽  
Author(s):  
M Le Thierry d'Ennequin ◽  
O Panaud ◽  
S Brown ◽  
S Siljak-Yakovlev ◽  
A Sarr
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content

scholarly journals The Preference of Direct or Indirect Methods in Preparing the Statement of Cash Flows in Decision Making: An Academic Perspective

2016 ◽  
Vol 8 (2) ◽  
pp. 206
Author(s):  
Abdullah, S. Hardan ◽  
Majed, A. Qabajeh ◽  
Aymen, M. Alshanti
Keyword(s):  
Decision Making ◽  
Indirect Method ◽  
Direct Method ◽  
Cash Flows ◽  
Decision Makers ◽  
Academic Rank ◽  
Indirect Methods ◽  
Statement Of Cash Flows ◽  
Qualitative Characteristics ◽  
Academic Sector

Two methods are used when reporting cash flows from operating activities: the direct method or the indirect method, both are acceptable from IAS with a preference of direct method. Thus, this paper examines which method of reporting the statement of cash flows provides useful information the decision makers rely on for decision making purposes. To achieve this aim, participants were selected from academic sector represented by universities professors. The study is based on the conceptual framework: qualitative characteristics of accounting information. To be useful, information must be relevant and represents faithfully what it claims to represent. In order to distinguish more useful financial information from those less useful, enhancing qualitative characteristics were examined. Results show that academic professors provide support for direct method of reporting cash flows over indirect method. The study sought to determine the effect of academic rank on these results. Evidence reveals that full and associate professors endorsed the preference of direct method more than assistant professors and lecturers. These results recommend the legislative bodies and entities to adopt the direct method in preparation the statement of cash flows.

Stage D1 Prostatic Adenocarcinoma: Significance of Nuclear DNA Ploidy Patterns Studied by Flow Cytometry

1988 ◽  
Vol 63 (2) ◽  
pp. 103-112 ◽  
Author(s):  
HARRY Z. WINKLER ◽  
LESLIE M. RAINWATER ◽  
ROBERT P. MYERS ◽  
GEORGE M. FARROW ◽  
TERRY M. THERNEAU ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nuclear Dna ◽  
Dna Ploidy ◽  
Prostatic Adenocarcinoma
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