scholarly journals Flow cytometric analysis of DNA content for ploidy determination in human solid tumors.

1979 ◽  
Vol 27 (1) ◽  
pp. 505-507 ◽  
Author(s):  
B Barlogie ◽  
W Göhde ◽  
B Drewinko
Keyword(s):  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Flow Chamber ◽  
Staining Procedure ◽  
Specific Staining ◽  
Flow Cytometric ◽  
Human Granulocytes ◽  
Human Solid Tumors ◽  
Repeat Examination ◽  
Cellular Dna

Flow cytometric studies of cellular DNA content were conducted in 26 patients with a variety of neoplasms. Cell dispersal was achieved with pepsin treatment, and a combination of ethidium bromide and mithramycin was used as DNA specific staining procedure. All measurements were conducted with a new sheath flow chamber in a PHYWE ICP 11 pulse cytophotometer. All but one patient with multiple myeloma had unimodal tumor cell DNA distributions. With human granulocytes as reference standard, 24 of 26 tumors were aneuploid; and of these, 23 showed varying degrees of hyperdiploidy. Except for one patient, ploidy abnormalities were stable on repeat examination.

Flow cytometric analysis in colorectal carcinoma: prognostic significance of cellular DNA content

1990 ◽  
Vol 5 (4) ◽  
pp. 223-227 ◽  
Author(s):  
A. Schillaci ◽  
D. D. Tirindelli ◽  
M. Ferri ◽  
L. Teodori ◽  
F. Mauro ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Dna Content ◽  
Prognostic Significance ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cellular Dna

Flow cytometric analysis of cellular DNA content in epithelial ovarian tumor

1992 ◽  
Vol 4 (2) ◽  
pp. 33-38
Author(s):  
Hongwu Wen ◽  
Shuwen Liu ◽  
Yongyan Mai ◽  
Renying Yan ◽  
Zhuxuan Shen
Keyword(s):  
Dna Content ◽  
Ovarian Tumor ◽  
Flow Cytometric Analysis ◽  
Epithelial Ovarian Tumor ◽  
Flow Cytometric ◽  
Cellular Dna

Flow cytometric analysis of cellular DNA content as an adjunct to the diagnosis of ovarian tumours of borderline malignancy

Pathology ◽  
1984 ◽  
Vol 16 (3) ◽  
pp. 301-306 ◽  
Author(s):  
Michael L. Friedlander ◽  
P. Russell ◽  
I.W. Taylor ◽  
D.W. Hedley ◽  
M.H.N. Tattersall
Keyword(s):  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Ovarian Tumours ◽  
Borderline Malignancy ◽  
Cellular Dna

Flow cytometric analysis of cellular DNA content in paraffin wax-embedded specimens of canine mammary tumours

1991 ◽  
Vol 105 (1) ◽  
pp. 75-82 ◽  
Author(s):  
E. Scanziani ◽  
M. Caniatti ◽  
S. Sent ◽  
E. Erba ◽  
F. Cairoli ◽  
...  
Keyword(s):  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Paraffin Wax ◽  
Flow Cytometric ◽  
Mammary Tumours ◽  
Cellular Dna

Prediction of long-term survival by flow cytometric analysis of cellular DNA content in patients with advanced ovarian cancer.

1988 ◽  
Vol 6 (2) ◽  
pp. 282-290 ◽  
Author(s):  
M L Friedlander ◽  
D W Hedley ◽  
C Swanson ◽  
P Russell
Keyword(s):  
Ovarian Cancer ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Figo Stage ◽  
Good Prognosis ◽  
Stage Iii ◽  
Favorable Prognosis ◽  
Flow Cytometric ◽  
Low Malignant Potential ◽  
Cellular Dna

The prognostic value of cellular DNA content in ovarian cancer (malignant common epithelial tumors) was investigated by flow cytometric analysis of paraffin-embedded tumor blocks from 128 previously untreated patients with International Federation of Gynecology and Obstetrics (FIGO) stage III and IV ovarian cancer entered in a prospective clinical trial of combination v sequential therapy with chlorambucil and cisplatin. Seventy-three percent of tumors were aneuploid and 27% were diploid. Multivariate analysis using a Cox model showed that cellular DNA content (P less than .001) and FIGO stage (P less than .02) were the only significant independent prognostic variables. The median survival was 13 months for patients with aneuploid tumors and 60 months for patients with diploid tumors (P less than .0001). Further analysis indicated that the good prognosis associated with diploid tumors was limited to patients with stage III disease, all patients with stage IV (spread beyond the peritoneal cavity or liver metastases) disease having a poor prognosis irrespective of ploidy. On pathological review, nine borderline ovarian tumors (of low malignant potential) were identified, and seven of these were diploid. These tumors have an unusually favorable prognosis, despite apparent dissemination within the peritoneal cavity, a paradox which is often difficult to explain using conventional histological criteria. Although the vast majority of tumors in this study (93%) were classified as invasive epithelial ovarian cancers, it is possible that some of the patients with stage III diploid tumors may have had malignancies that were predominantly of low malignant potential, thus accounting in part for the prognostic significance of DNA content. By incorporating flow cytometric DNA analysis with careful histopathological assessment, it may be possible to better identify patients with an inherently good prognosis. This assumes particular importance, as the relatively favorable prognosis of patients with stage III diploid ovarian tumors appears to be independent of the type of treatment.

Flow cytometric analysis of cellular DNA content in ovarian cancer

1990 ◽  
Vol 37 (2) ◽  
pp. 219-223 ◽  
Author(s):  
Kinya Hamaguchi ◽  
Haruo Nishimura ◽  
Tadashi Miyoshi ◽  
Kenichi Miyahara ◽  
Nobumasa Tateno ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Dna Content ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Cellular Dna

Flow cytometry in biodiversity surveys: methods, utility, and constraints

1997 ◽  
Vol 18 (1) ◽  
pp. 1-13 ◽  
Author(s):  
Ilya S. Darevsky ◽  
Robert W. Murphy ◽  
Ross D. MacCulloch ◽  
Cheryl Smith ◽  
Nikolai Orlov ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Liquid Nitrogen ◽  
Dna Content ◽  
Storage Temperature ◽  
Flow Cytometric Analysis ◽  
Flow Cytometric ◽  
Allopatric Species ◽  
Coefficients Of Variation ◽  
Solution Field ◽  
Cellular Dna

AbstractFlow cytometry of blood is a powerful tool for rapidly sorting individual specimens on the basis of cellular DNA content. During biodiversity surveys, the method enabled the early identification of both cryptic sympatric and allopatric species of Vietnamese ranid frogs. This method may be extremely valuable in sorting individuals from other taxa and geographic regions, especially when cellular DNA content is known to vary among closely related taxa, and in tropical situations where crypsis is a relatively common phenomenon. Protocols for preparation of freezing solution, field procedures, preparation of reference standards, and flow cytometric analysis are provided. The best method for field preservation of blood is freezing in liquid nitrogen; field fixation of blood in ethanol was less efficient and resulted in drastically increased coefficients of variation. Once samples have been transferred to freezer storage, they should not be returned to a lower storage temperature in liquid nitrogen.

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