scholarly journals Cytomat-R: a computer-controlled multiple laser source multiparameter flow cytophotometer system.

1977 ◽  
Vol 25 (7) ◽  
pp. 836-844 ◽  
Author(s):  
H M Shapiro ◽  
E R Schildkraut ◽  
R Curbelo ◽  
R B Turner ◽  
R H Webb ◽  
...  

A multiple illumination wavelength multiparameter flow cytophotometer system, using laser sources and controlled by a small, general-purpose digital computer, has been produced for use in the development of new flow cytometric techniques. Three different laser wave-lengths can be used simultaneously to illuminate different regions of the flow chamber; as many as five measurements of light scattering at various angles, extinction, and fluorescence at one or more wavelengths can be made at each illuminated station. Cells in suspension may be examined at rates of 1000 cells/sec, with seven correlated optical measurements being recorded for each cell. A library of programs for data manipulation and statistical analysis make it possible to use the system to develop and implement cell characterization, counting and classification procedures for basic and clinical research applications.

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Hazem M. El-Hageen ◽  
P.G. Kuppusamy ◽  
Aadel M. Alatwi ◽  
M. Sivaram ◽  
Z. Ahamed Yasar ◽  
...  

AbstractDifferent types of laser source modulation techniques have been used in various applications depending on the objective. As optical systems extract the laws and the best solutions from experiments and simulations, the present study uses simulation software with different modulation types so the output signals can be compared. The modulators used are Mach-Zehnder, which is an external modulator, and electro-absorption modulator and laser rate equation modulator, which are direct modulators. All these types have an optical link multimode (MM) fiber with a photodiode in the receiver end that can be modeled. The input and output signals are analyzed using different types of modulations.


1976 ◽  
Vol 24 (1) ◽  
pp. 388-395 ◽  
Author(s):  
R Curbelo ◽  
E R Schildkraut ◽  
T Hirschfeld ◽  
R H Webb ◽  
M J Block ◽  
...  

A general-purpose multiparameter flow cytophotometry system has been developed for use in the desgin of flow cytophotometers to perform specific tasks in automated cytology. Five separate measurement stations spaced along the axis of a capillary tube can be used to make up to eight optical measurements of individual cells flowing through the capillary. The system uses a broad-band arc source and can measure light scattered at various angles, light absorption by cell constituents and/or dyes and fluorescence of cell constituents and/or fluorochromes, excited directly and/or by energy transfer from neighboring molecules. High numerical aperture optics are used to maximize light-gathering capacity and minimize the effects of cell orientation and eccentricity of position in the fluid stream on measurements. A hard-wired preprocessor is used to detect the presence of cells and adjust sampling timing for changes in cell velocity; the electronic system also controls the gain of the detector photomultiplier tubes to compensate for background variations. Data acquistion and analysis are controled by a small general-purpose digital computer. The system has been used to develop a method and apparatus for blood cell counting and classification.


Author(s):  
Frank Kubacki ◽  
Dirk Hauschild ◽  
Mikhail Ivanenko ◽  
Jens Meinschien ◽  
Andreas Bayer ◽  
...  

High power laser sources are used in various production tools for cutting, welding and hardening of metal parts and patterning, annealing and lithography of flat panel displays, solar cells and microelectronic devices. Beside the right choice of the laser source suitable high performance optical beam delivery and shaping systems are needed for generating the appropriate beam profile and intensity distribution are of high importance for the right processing speed, quality and yield. In addition to the typical laser processes with circular beam shapes LIMO has developed laser sources with line shaped beams for large area processing for e.g. crystallization and tempering of conducting and semi-conducting films on glass for FPD, PV and thermal processing of semiconductor wafer, coated float glass and sheet metal. Due to the high power density of several 100kW/cm2 and line length up to several hundred millimetres a treatment capacity of several m2 per minute and processing speeds up to 1 m/s can be achieved per laser head with typical scan & repeat processes. The use of multiple laser heads in one machine scales the productivity to the individual needs. The high scanning speed together with line widths of 0,01mm to 0,1mm is the basis for heating only a few microns of the surfaces layers and no costly cooling time is needed like with regular heating technologies. With this controlled surface heating even more sensitive materials can be processes like inks on polymers and paper for RFIDs, printed solar absorbers and coatings. For industrial applications equally important is an adequate understanding of the physics of the light-matter interaction behind the process. In advance simulations of the tool performance can minimize technical and financial risk as well as lead times for prototyping and introduction into series production. Based on this knowledge together with a unique free-form micro-lens array production technology and patented micro-optics beam shaping designs a number of novel production tool sub-systems have been built by LIMO: 1. a multi-kilowatt direct diode illumination modules for solar cell annealing, and crystallization; 2. a novel green laser beam line for the annealing of silicon thin films on glass; 3. a novel wavefront shaping optics that generates a top hat beam profile from a TEM00 high-power laser source for accurate thin film structuring. For each of these sub-system basic functionalities, design principles and performance results are presented with a special emphasis on resilience, cost reduction and process reliability.


Author(s):  
Diego Liberati

In dealing with information it often turns out that one has to face a huge amount of data, often not completely homogeneous and often without an immediate grasp of an underlying simple structure. Many records, each one instantiating many variables, are usually collected with the help of various technologies. Given the opportunity to have so many data not easy to correlate by the human reader, but probably hiding interesting properties, one of the typical goals one has in mind is to classify subjects on the basis of a hopefully reduced meaningful subset of the measured variables. The complexity of the problem makes it worthwhile to resort to automatic classification procedures. Then, the question arises of reconstructing a synthetic mathematical model, capturing the most important relations between variables, in order to both discriminate classes of subjects and possibly also infer rules of behaviours that could help identify their habits. Such interrelated aspects will be the focus of the present contribution. The data mining procedures that will be introduced in order to infer properties hidden in the data are in fact so powerful that care should be put in their capability to unveil regularities that the owner of the data would not want to let the processing tool discover, like for instance, in some cases the customer habits investigated via the usual smart card used in commerce with the apparent reward of discounting. Four main general purpose approaches will be briefly discussed in the present article, underlying the cost effectiveness of each one. In order to reduce the dimensionality of the problem, simplifying both the computation and the subsequent understanding of the solution, the critical issues of selecting the most salient variables must be addressed. This step may already be sensitive, pointing to the very core of the information to look at. A very simple approach is to resort to cascading a divisive partitioning of data orthogonal to the principal directions (PDDP) (Boley, 1998) already proven to be successful in the context of analyzing micro-arrays data (Garatti, Bittanti, Liberati, & Maffezzoli, 2007). A more sophisticated possible approach is to resort to a rule induction method, like the one described in Muselli and Liberati (2000). Such a strategy also offers the advantage to extract underlying rules, implying conjunctions or disjunctions between the identified salient variables. Thus, a first idea of their even nonlinear relations is provided as a first step to design a representative model, whose variables will be the selected ones. Such an approach has been shown (Muselli & Liberati, 2002) to be not less powerful over several benchmarks than the popular decision tree developed by Quinlan (1994). An alternative in this sense can be represented by Adaptive Bayesian networks (Yarmus, 2003) whose advantage is also to be available on a commercial wide spread data base tool like Oracle. Dynamics may matter. A possible approach to blindly build a simple linear approximating model is thus to resort to piece-wise affine (PWA) identification (Ferrari-Trecate, Muselli, Liberati, & Morari, 2003). The joint use of (some of) such four approaches briefly described in this article, starting from data without known priors about their relationships, will allow to reduce dimensionality without significant loss in information, then to infer logical relationships, and, finally, to identify a simple input-output model of the involved process that also could be used for controlling purposes, even those potentially sensitive to ethical and security issues.


2020 ◽  
Vol 2020 ◽  
pp. 1-6
Author(s):  
Haibin Sun ◽  
Tingting Liu

The fringe projection technique has been widely used in optical measurements. In this paper, we demonstrate a scheme to measure the 3D displacement of a deformed sample using Talbot fringe projection. In this process, we designed a two-dimensional square Talbot hologram. In this approach, we used the basic principle of triangulation, and a computer-controlled liquid crystal spatial light modulator (LC-SLM) was placed in the optical path. The Talbot array hologram was displayed on the LC-SLM screen and projected onto the surface of a sample. Two patterns were recorded: one before and one after deformation. We simultaneously acquired the in-plane and out-of-plane displacements using the digital image correlation (DIC) method. This scheme is simple and easily implemented. Theoretical and experimental results are presented.


2019 ◽  
Vol 10 (2) ◽  
pp. 151-159
Author(s):  
V. A. Alekseev ◽  
M. R. Zaripov ◽  
A. S. Perminov ◽  
E. A. Sitnikova ◽  
V. P. Usol’tsev ◽  
...  

At the present time, developing of autonomous laser systems requires increasing of the output power of the laser sources used in composition of those systems and at the same time reducing of the energy usage in the system. The possibility of increasing output peak power of pulsed laser sources by using the method of synchronous non-coherent beam combining in ring fiber-optic delay line is considered by authors. Objective of this work was estimating energy effectiveness of laser systems, which based on this method.General constructing method of the laser pulsed laser source with ring fiber delay line is considered, its block diagram and the general operating principle of similar systems are presented. Two versions of laser systems based on the described method of beam combining are presented: using an optical combiner and an optical switch; using fiber welding instead of a combiner and an optical switch. The graphical dependence of the energy effectiveness on the number of circulations in ring fiber-optic delay line is obtained for both versions of laser systems.As a result of the analysis of the considered devices operation, it was shown that considered systems allow to obtain increasing the peak power of a laser pulse without increasing the power supply, also the system, that use welded fi instead of the optical combiner, has greater effi than system with optical combiner.


2021 ◽  
Author(s):  
huseyin ozgur kazanci ◽  
Kiichi Niitsu

Abstract The variation of photon fluence distributions [photon/cm2.s] for different input angle laser sources was shown by researchers experimentally [1]. According to this philosophy, different input angle source and detector photon entrance from tissue surface into imaging media have different photon fluence distributions for a specific tissue type. In this study, different input angle simulations were used for pulsed laser photons which uses time resolved (TR) Monte Carlo (MC) photon-tissue interaction simulation program to prove the philosophy in TR run mode. TR run mode MC simulation program trmc.c [2] was modified and used to generate TR photon counts inside the homogenous simulation environment. It has homogeneous tissue optical properties, absorption μa = 0.1 cm-1, scattering μs = 100 cm-1, and anisotropy g = 0.90 coefficients. Multi-input angle philosophy was first demonstrated by the researchers [1]. It was defined and experimentally proven. Photon fluencies which are forward model weight matrix coefficients differences were successfully shown for TR laser as a general procedure. In this study, differences were drawn for seven different input angle sources with pulsed laser photons. The proof-of-concept philosophy was shown successfully. The purpose of the use of pulsed laser is to show the righteousness of the philosophy in TR run mode, since the TR diffuse optical tomography (TRDOT) device would be made as a biomedical optic imaging (BOI) device. Cylindrical radial coordinate system which was defined in trmc.c [2] in earlier was used, the code was modified, and photon fluencies were generated based on the different input angle laser photons. Cylindrical coordinate system has 1 cm and 36-element radial r, and depth z grids. 100.000 photons were sent from pencil beam tissue surface point. Photons would be thought as group of ultra-narrow band pulsed laser photons. The main purpose of showing photon fluencies for different laser source input angles were succeeded and image reconstruction procedure was also applied. Ten time series were used which are [4, 8, 12, 16, 22, 26, 30, 38, 46, 52] picoseconds (ps). Different input angle photon fluence distribution figures were drawn. These are 0º, 15º, 30º, 45º, 60º, 75º, 90º. Photon fluence differences were also drawn and observed for different input angle laser sources. Forward model problem different input angle laser source and detector transfer functions were also drawn. Finally inlusion was embedded inside the homogenous simulation environment and images were reconstructed for both scenarios and localization error (LE), and concentration error (CE) was calculated for both scenarions and compared with each other.


Volume 3 ◽  
2004 ◽  
Author(s):  
Paola Bassani ◽  
Edoardo Capello ◽  
Enrico Gallus ◽  
Elisabetta Gariboldi ◽  
Luca Longoni ◽  
...  

In the recent decade the laser drilling process has continually attracted new interests and has found increasing applications in the industry. Nowadays the most common industrial laser sources for laser drilling are the solid state ones with pulse length of the order of milliseconds, even if nanosecond and femtosecond pulse sources can also be found in industrial applications. The latter, short and ultrashort laser sources, are very promising since they are expected to generate the hole directly by vaporisation, leaving the hole surfaces free of molten and resolified layer, as well as very low amount of spatter. This paper reports an experimental study on microhole laser machining and on influence of some process parameters on the hole shape. The laser source used in the study was a pulsed, diode pumped, Q-switched Nd:YAG laser. The materials investigated were four different alloys (AISI 304 stainless steel, cp titanium, CuZn35 brass and IN718 alloy), provided as 0.5 mm thick commercial sheets. Since an experimental approach has been here preferred, the performed experimental plan has been designed by the analysis of variance technique. The influence of material type, as well as laser process parameters, like pulse frequency and pulse energy, have been investigated. Relevant geometrical features, like top and bottom hole diameter, taper angle, top and bottom aspect ratio, have been measured and analysed. Moreover, the geometric features of the top spatter as well as the metallurgical characteristics of heat affected zone has been investigated. The results have shown that pulse energy strongly affects both geometric and metallurgical hole features. On the other hand, pulse frequency does not seem to influence the hole shape in all material. The presence of spatter and metallographic analysis confirmed the production of molten layers in all material mainly around the hole entrance.


2014 ◽  
Vol 0 (0) ◽  
Author(s):  
Veerpal Kaur ◽  
Kamaljit Singh Bhatia ◽  
Amrit Kaur

AbstractIn this paper, the dominating effect of four wave mixing is analysed with line width of laser source in wave length division multiplexed system. Power of FWM signal is minimum, Q factor maximum and bit error rate is minimized at optimized value of line width at all laser source power.


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